Intangiriro yo gukusanya serumu
1. Mubisanzwe, umuyoboro wamaraso ufite ingofero yumutuku yerurutse, kandi ntabubasha bwo kubika amaraso.Ikoreshwa mubutaka bwibanze bwamaraso biochemie, banki yamaraso hamwe nibizamini bijyanye na serologiya.
2. Umutwe wa orange-umutuku wumuvuduko wamaraso wihuta, hamwe numuyoboro wo gukusanya amaraso urimo coagulant, ishobora gukurura fibre fibre trypsin, kugirango aside irike yubusa ibe polimer acide idasembuye, bityo ikabyara neza aside irike yubusa.Umuyoboro wamaraso wihuta urashobora guhunika amaraso yakusanyijwe muminota 5, kandi birakwiriye mubushakashatsi rusange bwamaraso.
3. Inert yo gutandukanya gel hamwe na coagulation yihuta yumuvuduko wumutwe wumuhondo wumuhondo, hamwe na gel yo gutandukanya inert hamwe na coagulant byongewe mumiyoboro yo gukusanya amaraso.Icyitegererezo kimaze gukusanywa no kuyungurura, gel yo gutandukanya inert irashobora gutandukanya burundu ibice byamazi (selile yamaraso cyangwa amaraso) nibice bikomeye (selile yamaraso, ibara ryamaraso yera, platine, aside irike yubusa, nibindi) mumaraso kandi rwose ubishyire hagati ya IVF kugirango ukore inzitizi karemano., Icyegeranyo cyikitegererezo gikomeza igihe kinini mumasaha 48.Coagulant irashobora kubyutsa vuba imikorere yimitsi yamaraso kandi ikihutisha inzira ya coagulation, ikwiranye nubushakashatsi bwibinyabuzima bwa biohimiki.
4. Umuyoboro wa Heparin anticoagulant ufite icyatsi kibisi cya zeru, na heparin yongerwa mumiyoboro yo gukusanya amaraso.Heparin ihita igira ingaruka za antithrombine, ishobora kongera igihe cyo kwambara cyo gukusanya ingero.Irakwiriye kwipimisha ingirabuzimafatizo zamaraso, gusesengura gazi yamaraso, gupima hematocrit, igipimo cyimitsi ya erythrocyte no gupima ingufu rusange za biohimiki.Ntibikwiye kwipimisha amaraso.Heparine nyinshi cyane izatera kwirundanya kwamaraso yera kandi ntishobora gukoreshwa mukubara selile yera.Irashobora gutuma ibice byamaraso bisa nubururu bwerurutse nyuma yo kurangi, ntibikwiye rero gutondekanya selile yera.
5. Umuyoboro wo gutandukanya amaraso ufite igifuniko cyicyatsi kibisi.Ongeramo lithium heparin anticoagulant kuri inert itandukanya reberi kugirango ugere ku ntego yo gutandukanya amaraso vuba.Nuburyo bwiza bwo gupima igisubizo cya electrolyte, kandi birashobora no gukoreshwa mugupima ibinyabuzima byibanze byamaraso no gupima amaraso y’ibinyabuzima nka ICU.Ikusanyirizo ryamaraso rishobora gukorerwa ako kanya kuri mashini kandi rikagumaho amasaha 48 mugihe gikonje.
6 kubangamira no guhagarika inzira ya coagulation ya endogenous cyangwa exogenous, bityo ukirinda kwishyira hamwe kwamaraso.Birakwiriye kwipimisha muri rusange.Kubura amahwemo bikoreshwa mugupima imikorere ya coagulation hamwe na test ya imikorere ya platel.Kubura amahwemo bikoreshwa kandi kuri sodium ion, calcium ion, potasiyumu ion, ferrous ion, fosifata ya alkaline, creatine kinase na leucine hydroxy peptide Enzyme gupima hamwe nubushakashatsi bwa PCR.
7. Sodium citrate yamaraso coagulation imikorere test tube yoroheje yubururu.Sodium citrate ishingiye ahanini kuri chelating hamwe na sodium ion mumaraso kugirango igire ingaruka mbi.Bikoreshwa mubizamini byimikorere ya coagulation, agaciro ka anticoagulant yibanze cyane hamwe nishyirahamwe ryigihugu rya Clinical Laboratory Standardization (NCCLS) ni 3.2% cyangwa 3.8% (bihwanye na 0.109mol / L cyangwa 0.129mol / L).Ikigereranyo ni 1: 9.
8. Sodium citrate erythrocyte igipimo cyibipimo byo gupima igipimo cyumutwe wumukara-umukara.Agaciro ka sodium citrate yagaragajwe mugupima igipimo cya erythrocyte ni 3.2% (bihwanye na 0.109mol / L), igipimo cya anticoagulant namaraso ni 1: 4.
Igihe cyo kohereza: Ukuboza-13-2021
