ʻO ka hōʻiliʻili ʻana i ke koko he ʻohi koko ʻino ʻole, ʻo ka hoʻohana ʻana i ka nui o nā mea kani a me ka mālama ʻana i ke koko i mua o nā koi kiʻekiʻe e pili ana i ke kūpaʻa o nā kumu koko kumu, no laila ua haki ka ʻenehana hōʻiliʻili koko i nā koi o ka palekana wale nō. .Hiki ke hoʻohana ʻia ka pololei, ke ʻano kumu o ka specimen, ka manawa mālama, kūpono o ka mīkini paipu a me ka ikaika o ka pahu hoʻāʻo e loiloi i ka maikaʻi o ka hōʻiliʻili ʻana.
ʻO ka hoʻokaʻawale ʻana o ka ʻohi koko vacuum
1. Hoʻohana ʻia ka paipu serum maʻamau me ka pāpale ʻulaʻula a me ka moku hōʻiliʻili me ka ʻole o nā mea hoʻohui no ka maʻamau serum biochemical, panakō koko a me nā hoʻokolohua serological.
2. He coagulant ka ipu serum wikiwiki me ka pāpale ʻalani i loko o ka paipu hōʻiliʻili koko e hoʻoikaika i ke kaʻina hana coagulation.Hiki i ka ipu serum wikiwiki ke coagulate i ke koko i hōʻiliʻili ʻia i loko o 5 mau minuke, a kūpono ia no ka hoʻāʻo serialization serological i ka pilikia.
3. Hoʻohui ʻia ke kāpili hoʻokaʻawale inert a me ka coagulant booster i loko o ka paipu hōʻiliʻili koko me ka pāpale gula.Ma hope o ka centrifuged o ka specimen, hiki i ka inert separator gel ke hoʻokaʻawale loa i nā ʻāpana wai (serum a i ʻole plasma) a me nā ʻāpana paʻa (ke koko ʻulaʻula, nā keʻokeʻo keʻokeʻo, platelets, fibrin, etc.) i loko o ke koko a hōʻuluʻulu loa i waenakonu o ka. ka paipu ho'āʻo e hana i keakea, a paʻa ka specimen i loko o 48 hola.Hiki i ka Coagulant ke hoʻoikaika koke i ka mīkini coagulation, hoʻolalelale i ke kaʻina hana coagulation, kūpono no ka hoʻāʻo biochemical serum pilikia.
4. ʻO ka heparin anticoagulant tube he pāpale ʻōmaʻomaʻo a hoʻohui ʻia ka heparin i ka paipu hōʻiliʻili koko.Loaʻa i ka Heparin ka hopena o ka antithrombin a hiki ke hoʻolōʻihi i ka manawa cotting o nā specimen.He kūpono no ka hoʻāʻo ʻana i ka erythrocyte brittleness, ka nānā ʻana i ke kinoea koko, ka hoʻāʻo hematocrit, ka sedimentation erythrocyte a me ka hoʻoholo biochemical maʻamau, ʻaʻole kūpono no ka hoʻāʻo coagulation koko.Hiki i ka nui o ka heparin ke hoʻoulu i ka hōʻiliʻili o nā keʻokeʻo keʻokeʻo a ʻaʻole hiki ke hoʻohana ʻia no ka helu ʻana o ke koko keʻokeʻo.ʻAʻole kūpono ia no ka hoʻokaʻawale ʻana i nā leukocyte no ka mea hiki iā ia ke hana i ka ʻulaʻula ʻulaʻula o ke koko koko.
5. ʻO ka paipu hoʻokaʻawale plasma me ka pāpale ʻōmaʻomaʻo māmā, hoʻohui i ka heparin lithium anticoagulant i loko o ka inert separation hose hiki ke hoʻokō i ke kumu o ka hoʻokaʻawale plasma wikiwiki, ʻo ia ke koho maikaʻi loa no ka ʻike electrolyte, a hiki ke hoʻohana ʻia no ka hoʻoholo ʻana i ka biochemical plasma maʻamau a me ka plasma pilikia. ʻike biochemical ma ICU.Hiki ke hoʻouka pololei ʻia nā laʻana plasma i loko o ka mīkini a noho paʻa i loko o kahi waiho anu no 48 mau hola.
6. EDTA anticoagulation tube poni pāpale, EDTA (EDTA, molecular kaumaha 292) a me kona paakai he 'ano o ka amino polycarboxylic acid, i hiki pono chelate calcium iona i loko o ke koko samples.ʻO ka Chelating calcium a i ʻole ka wehe ʻana i ka wahi hoʻohālikelike calcium e poloka a hoʻopau i ke kaʻina hana coagulation endogenous a i ʻole exogenous, i mea e pale ai i ka coagulation o nā laʻana koko.He kūpono no ka ho'āʻo hematology maʻamau, ʻaʻole kūpono no ka hoʻāʻo coagulation a me ka hoʻāʻo ʻana o ka platelet function, ʻaʻole kūpono no ka calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase a me leucine aminopeptase hoʻoholo, kūpono no ka hoʻāʻo PCR.
7. Sodium citrate coagulation ho'āʻo paipu polū polū, sodium citrate nui ma o ka chelating me ka calcium iona i loko o ke koko samples e pāʻani i ka anticoagulant hopena.Hoʻohana ʻia i nā hoʻāʻo coagulation, ua ʻōlelo ʻia ke Kōmike National no Clinical Laboratory Standards, NCCLS) i nā ʻano anticoagulant o 3.2% a i ʻole 3.8% (e like me 0.109mol/L a i ʻole 0.129mol/L) a me kahi anticoagulant 1:9 i ke koko ratio.
8. Sodium citrate esR tube ʻeleʻele pāpale, esR nā koi hoʻāʻo o ka sodium citrate concentration he 3.2% (e like me 0.109mol/L) anticoagulant i ke koko ratio o 1:4.
Potassium oxalate/sodium fluoride poʻo hina, sodium fluoride he anticoagulant nāwaliwali, maʻamau me ka potassium oxalate a i ʻole ka sodium iodate i hui pū ʻia, ka ratio o ka sodium fluoride 1, potassium oxalate 3. ʻO kēia hui ʻana o 4mg ka mea e pale ai i ka 1ml koko mai ka clotting a kāohi i ka glycolysis no 23 lā.He mea mālama maikaʻi ia no ke ana ʻana i ka glucose o ke koko a ʻaʻole pono e hoʻohana ʻia no ka hoʻoholo ʻana i ka urease o ka urea, ʻaʻole no ka alkaline phosphatase a me ka amylase hoʻoholo.Manaʻo ʻia no ka hoʻāʻo ʻana i ka glucose koko.
Ka manawa hoʻouna: Nov-10-2021
