Amashubhu okuqoqa igazinge-anticoagulant ku-tube
1 Amashubhu eqoqo legazi aqukethe i-sodium heparin noma i-lithium heparin: I-Heparin iyi-mucopolysaccharide equkethe iqembu le-sulfate ene-negative charge enamandla, enomthelela wokuqinisa i-antithrombin III ukuze yenze i-serine protease ingasebenzi, ngaleyo ndlela ivimbele Ukwakheka kwe-thrombin, futhi inemiphumela ye-anticoagulant njengokuvimbela. ukuhlanganiswa kweplatelet.Amashubhu e-Heparin ngokuvamile asetshenziselwa ukuthola isimo esiphuthumayo se-biochemical nokugeleza kwegazi, futhi ayisinqumo esingcono kakhulu sokutholwa kwe-electrolyte.Lapho kuhlolwa i-sodium ion kumasampula egazi, i-heparin sodium akufanele isetshenziswe, ukuze ingaphazamisi imiphumela yokuhlolwa.Futhi ayikwazi ukusetshenziselwa ukubala nokuhlukaniswa kwe-leukocyte, njengoba i-heparin ingabangela ukuhlanganiswa kwe-leukocyte.
2 Amashubhu okuqoqwa kwegazi aqukethe i-EDTA nosawoti wayo (EDTA—): I-EDTA iyi-amino polycarboxylic acid, engahlanza kahle ama-ion e-calcium egazini, futhi i-calcium e-chelating izosusa i-calcium ku-calcium.Ukususwa kwephuzu lokusabela kuzovimbela futhi kuqede inqubo ye-endogenous noma yangaphandle ye-coagulation, ngaleyo ndlela kuvimbele ukuhlangana kwegazi.Uma iqhathaniswa namanye ama-anticoagulant, inethonya elincane ekuhlanganiseni kwamangqamuzana egazi kanye nokwakheka kwamangqamuzana egazi, ngakho usawoti we-EDTA uvame ukusetshenziswa.(2K, 3K, 2Na) njengama-anticoagulant.Isetshenziselwa ukuhlolwa kwegazi okujwayelekile, futhi ayikwazi ukusetshenziselwa ukuhlangana kwegazi, izakhi zokulandelela kanye nokuhlolwa kwe-PCR.
3 Amashubhu okuqoqa igazi aqukethe i-sodium citrate anticoagulant: I-sodium citrate idlala umphumela we-anticoagulant ngokusebenza ku-chelation ye-calcium ions kusampula yegazi.Isilinganiso se-ejenti negazi ngu-1: 9, futhi sisetshenziswa ikakhulukazi ohlelweni lwe-fibrinolytic (isikhathi se-prothrombin, isikhathi se-thrombin, isikhathi se-thrombin esiyingxenye esicushiwe, i-fibrinogen).Lapho uqoqa igazi, qaphela inani legazi eliqoqwe ukuze uqinisekise ukunemba kwemiphumela yokuhlolwa.Ngokushesha ngemva kokuqoqwa kwegazi, kufanele iguqulwe futhi ixutshwe izikhathi ezingu-5-8.
4 Iqukethe i-sodium citrate, ukugcwala kwe-sodium citrate ngu-3.2% (0.109mol/L) kanye no-3.8%, isilinganiso sevolumu ye-anticoagulant negazi ngu-1:4, ngokuvamile esetshenziselwa ukutholwa kwe-ESR, ingxenye ye-anticoagulant iphezulu kakhulu liphezulu, igazi liyahlanjululwa, elingasheshisa izinga le-erythrocyte sedimentation.
5 Ishubhu liqukethe i-potassium oxalate/sodium fluoride (ingxenye engu-1 ye-sodium fluoride nezingxenye ezi-3 ze-potassium oxalate): I-sodium fluoride iyi-anticoagulant ebuthakathaka, enomthelela omuhle ekuvimbeleni ukuwohloka koshukela wegazi, futhi iyisilondolozi esihle kakhulu sokuthola ushukela wegazi. .Ukunakekela kufanele kuthathwe ukuze uguqule futhi uxube kancane lapho usebenzisa.Ngokuvamile isetshenziselwa ukuthola ushukela osegazini, hhayi ekunqumeni i-urea ngendlela ye-urease, noma ekutholweni kwe-alkaline phosphatase ne-amylase.
Singakuhlinzeka ngemikhiqizo ehlobene.
Isikhathi sokuthumela: Aug-19-2022
