Blood collection tubes with anticoagulant in the tube
1 Blood collection tubes containing sodium heparin or lithium heparin: Heparin is a mucopolysaccharide containing a sulfate group with a strong negative charge, which has the effect of strengthening antithrombin III to inactivate serine protease, thereby preventing thrombin Formation, and has anticoagulant effects such as preventing platelet aggregation. Heparin tubes are generally used for emergency biochemical and blood flow detection, and are the best choice for electrolyte detection. When testing sodium ions in blood samples, heparin sodium should not be used, so as not to affect the test results. It also cannot be used for leukocyte counting and differentiation, as heparin can cause leukocyte aggregation.
2 Blood collection tubes containing EDTA and its salts (EDTA—): EDTA is an amino polycarboxylic acid, which can effectively chelate calcium ions in the blood, and chelating calcium will remove calcium from calcium. The removal of the reaction point will prevent and terminate the endogenous or extrinsic coagulation process, thereby preventing blood coagulation. Compared with other anticoagulants, it has less influence on the coagulation of blood cells and the morphology of blood cells, so EDTA salt is usually used. (2K, 3K, 2Na) as anticoagulants. It is used for general hematological examinations, and cannot be used for blood coagulation, trace elements and PCR examinations.
3 Blood collection tubes containing sodium citrate anticoagulant: Sodium citrate plays an anticoagulant effect by acting on the chelation of calcium ions in the blood sample. The ratio of agent to blood is 1:9, and it is mainly used in the fibrinolytic system (prothrombin time, thrombin time, activated partial thrombin time, fibrinogen). When collecting blood, pay attention to the amount of blood collected to ensure the accuracy of the test results. Immediately after blood collection, it should be inverted and mixed 5-8 times.
4 Contains sodium citrate, the concentration of sodium citrate is 3.2% (0.109mol/L) and 3.8%, the volume ratio of anticoagulant to blood is 1:4, generally used for ESR detection, the proportion of anticoagulant is too high When it is high, the blood is diluted, which can speed up the erythrocyte sedimentation rate.
5 The tube contains potassium oxalate/sodium fluoride (1 part of sodium fluoride and 3 parts of potassium oxalate): Sodium fluoride is a weak anticoagulant, which has a good effect on preventing blood sugar degradation, and is an excellent preservative for blood sugar detection. Care should be taken to invert and mix slowly when using. It is generally used for blood sugar detection, not for the determination of urea by urease method, nor for the detection of alkaline phosphatase and amylase.
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Post time: Aug-19-2022
