Kev faib tawm ntawm lub tshuab nqus tsevcov hlab ntsha
6. Heparin anticoagulation raj nrog lub hau ntsuab
Heparin tau ntxiv rau hauv cov hlab ntshav.Heparin ncaj qha muaj cov nyhuv antithrombin, uas tuaj yeem ua rau lub sijhawm coagulation ntawm cov qauv.Rau kev sim thiab biochemical feem ntau, xws li lub siab ua haujlwm, lub raum ua haujlwm, cov ntshav lipids, ntshav qab zib, thiab lwm yam. Nws yog tsim rau kev kuaj ntshav liab fragility, kuaj ntshav roj, kuaj hematocrit, erythrocyte sedimentation tus nqi thiab kev txiav txim siab dav dav biochemical, tab sis tsis. haum rau kev kuaj ntshav coagulation.Heparin ntau dhau tuaj yeem ua rau cov qe ntshav dawb sib sau ua ke thiab siv tsis tau rau kev suav cov qe ntshav dawb.Nws kuj tseem tsis tsim nyog rau kev faib cov leukocyte vim tias nws tuaj yeem ua rau cov ntshav cov yeeb yaj kiab stained nrog lub teeb xiav tom qab.Nws tuaj yeem siv rau ntshav rheology.Hom qauv yog plasma.Tam sim ntawd tom qab sau cov ntshav, thim rov qab thiab sib tov rau 5-8 zaug, thiab nqa cov ntshav siab rau kev siv.
7. Lub teeb ntsuab cap ntawm plasma cais raj
Ntxiv heparin lithium anticoagulant rau inert sib cais roj hmab raj tuaj yeem ua tiav lub hom phiaj ntawm kev sib cais sai ntawm cov ntshav.Rau kev sim thaum muaj xwm txheej ceev thiab biochemical feem ntau, xws li lub siab ua haujlwm, lub raum ua haujlwm, cov ntshav lipids, ntshav qab zib, thiab lwm yam. Cov ntshav kuaj ntshav tuaj yeem thauj khoom ncaj qha rau ntawm lub tshuab thiab ruaj khov rau 48 teev hauv tub yees.Nws tuaj yeem siv rau ntshav rheology.Hom qauv yog plasma.Tam sim ntawd tom qab sau cov ntshav, thim rov qab thiab sib tov rau 5-8 zaug, thiab nqa cov ntshav siab rau kev siv.
8. Potassium oxalate/sodium fluoride grey cap
Sodium fluoride yog ib qho tsis muaj zog tiv thaiv coagulant, uas feem ntau yog siv ua ke nrog potassium oxalate lossis sodium ethiodate, thiab nws qhov piv yog 1 feem ntawm sodium fluoride thiab 3 feem ntawm potassium oxalate.4mg ntawm qhov sib tov no tuaj yeem ua rau 1ml ntawm cov ntshav tsis coagulate hauv 23 hnub thiab inhibit qhov decomposition ntawm qab zib.Nws tsis tuaj yeem siv rau kev txiav txim siab ntawm urea los ntawm txoj kev urease, lossis rau kev txiav txim siab ntawm alkaline phosphatase thiab amylase.Nws raug pom zoo rau kev kuaj ntshav qab zib.Nws muaj sodium fluoride lossis potassium oxalate lossis disodium ethylenediaminetetraacetate (EDTA-Na) tshuaj tsuag, uas tuaj yeem cuam tshuam cov kev ua haujlwm enolase hauv cov piam thaj metabolism.Tom qab kos cov ntshav, invert thiab sib tov rau 5-8 zaug.Cov kua plasma yog tshwj tseg rau kev siv, thiab nws yog lub raj tshwj xeeb rau kev ntsuas ntshav qabzib sai.
9. EDTA anticoagulation raj ntshav cap
Ethylenediaminetetraacetic acid (EDTA, molecular yuag 292) thiab nws cov ntsev yog ib qho amino polycarboxylic acid, tsim nyog rau kev kuaj hematology, thiab yog cov kev ntsuam xyuas zoo rau cov ntshav niaj hnub, glycosylated hemoglobin, thiab cov ntshav kuaj ntshav.Tsis haum rau kev kuaj coagulation thiab platelet muaj nuj nqi, tsis yog rau kev txiav txim siab ntawm calcium ion, potassium ion, sodium ion, hlau ion, alkaline phosphatase, creatine kinase thiab leucine aminopeptidase, haum rau PCR kuaj.Txau 100ml ntawm 2.7% EDTA-K2 tov rau ntawm phab ntsa sab hauv ntawm lub tshuab nqus tsev, tshuab ziab ntawm 45 ° C, sau cov ntshav mus rau 2ml, rov qab thiab sib tov 5-8 zaug tam sim ntawd tom qab cov ntshav kos, thiab sib tov zoo rau kev siv tom qab.Hom qauv yog tag nrho cov ntshav, uas yuav tsum tau muab sib tov sib npaug ua ntej siv.
Post lub sij hawm: Mar-02-2022
