Ukuhlukaniswa kwe-vacuumimithambo yegazi
6. I-Heparin anticoagulation tube enekepisi eliluhlaza
I-Heparin yengezwe eshubhu lokuqoqa igazi.I-Heparin ngokuqondile inomphumela we-antithrombin, engandisa isikhathi sokuhlangana kwesampula.Okwezimo eziphuthumayo nokuhlolwa okuningi kwamakhemikhali e-biochemical, njengokusebenza kwesibindi, ukusebenza kwezinso, amafutha egazi, ushukela wegazi, njll. Ifanele ukuhlolwa kobuthakathaka bengqamuzana elibomvu legazi, ukuhlaziya igesi yegazi, ukuhlolwa kwe-hematocrit, izinga le-erythrocyte sedimentation kanye nokunqunywa kwe-biochemical okuvamile, kodwa hhayi. ilungele ukuhlolwa kwe-blood coagulation.I-heparin eyeqile ingabangela ukuhlangana kwamangqamuzana amhlophe egazi futhi ayikwazi ukusetshenziselwa ukubala amangqamuzana amhlophe egazi.Ayifanele futhi ukuhlukaniswa kwe-leukocyte ngoba ingenza ifilimu yegazi ingcoliswe ngengemuva eliluhlaza okwesibhakabhaka.Ingasetshenziselwa i-rheology yegazi.Uhlobo lwesampula yi-plasma.Ngokushesha ngemva kokuqoqwa kwegazi, guqula futhi uxube izikhathi ezingu-5-8, futhi uthathe i-plasma ephezulu ukuze uyisebenzise.
7. Isigqoko esiluhlaza esikhanyayo seshubhu lokuhlukanisa i-plasma
Ukwengeza i-heparin lithium anticoagulant kushubhu yerabha yokuhlukanisa i-inert ingafinyelela inhloso yokuhlukaniswa ngokushesha kwe-plasma.Ezimweni eziphuthumayo kanye nokuhlolwa okuningi kwamakhemikhali e-biochemical, njengokusebenza kwesibindi, ukusebenza kwezinso, amafutha egazi, ushukela wegazi, njll. Amasampula e-plasma angalayishwa ngokuqondile emshinini futhi azinze amahora angu-48 ngaphansi kwesiqandisi.Ingasetshenziselwa i-rheology yegazi.Uhlobo lwesampula yi-plasma.Ngokushesha ngemva kokuqoqwa kwegazi, guqula futhi uxube izikhathi ezingu-5-8, futhi uthathe i-plasma ephezulu ukuze uyisebenzise.
8. I-Potassium oxalate/i-sodium fluoride grey cap
I-sodium fluoride iyi-anticoagulant ebuthakathaka, evame ukusetshenziswa ngokuhambisana ne-potassium oxalate noma i-sodium ethiodate, futhi isilinganiso sayo siyingxenye engu-1 ye-sodium fluoride kanye nezingxenye ezi-3 ze-potassium oxalate.I-4mg yale ngxube ingenza u-1ml wegazi ungahlangani phakathi nezinsuku ezingama-23 futhi uvimbele ukubola koshukela.Awukwazi ukusetshenziselwa ukunqunywa kwe-urea ngendlela ye-urease, noma ekunqumeni i-alkaline phosphatase ne-amylase.Kunconywa ukuhlola ushukela wegazi.Iqukethe i-sodium fluoride noma i-potassium oxalate noma i-disodium ethylenediaminetetraacetate (EDTA-Na) spray, engavimbela umsebenzi we-enalase ku-glucose metabolism.Ngemuva kokudonsa igazi, guqula futhi uhlanganise izikhathi ezingu-5-8.I-plasma ewuketshezi ibekelwe ukusetshenziswa, futhi iyishubhu elikhethekile lokukala ushukela wegazi ngokushesha.
9. I-EDTA anticoagulation tube cap purple
I-Ethylenediaminetetraacetic acid (EDTA, i-molecular weight 292) kanye nosawoti bayo i-amino polycarboxylic acid, ilungele ukuhlolwa kwe-hematology evamile, futhi ingamashubhu okuhlola akhethwayo omjikelezo wegazi, i-glycosylated hemoglobin, nokuhlolwa kweqembu legazi.Ayifanele ukuhlolwa kwe-coagulation kanye nokuhlolwa kokusebenza kweplatelet, noma ukunqunywa kwe-calcium ion, i-potassium ion, i-sodium ion, i-iron ion, i-alkaline phosphatase, i-creatine kinase ne-leucine aminopeptidase, ifanele ukuhlolwa kwe-PCR.Fafaza u-100ml wesixazululo esingu-2.7% se-EDTA-K2 odongeni lwangaphakathi lweshubhu ye-vacuum, wome ku-45°C, uqoqe igazi libe ngu-2ml, uguqule bese uxuba izikhathi ezingu-5-8 ngokushesha ngemva kokudonsa igazi, bese uxuba kahle ukuze usetshenziswe kamuva.Uhlobo lwesampula yigazi eliphelele, elidinga ukuxutshwa ngokulinganayo ngaphambi kokusetshenziswa.
Isikhathi sokuthumela: Mar-02-2022
