Gulu la vacuumzotengera zosonkhanitsira magazi
6. Heparin anticoagulation chubu ndi wobiriwira kapu
Heparin anawonjezeredwa ku chubu chotolera magazi.Heparin mwachindunji imakhala ndi zotsatira za antithrombin, zomwe zimatha kutalikitsa nthawi ya coagulation ya chitsanzocho.Kwa zoyesera zadzidzidzi komanso zamankhwala amthupi, monga ntchito ya chiwindi, ntchito ya impso, lipids m'magazi, shuga wamagazi, ndi zina zambiri. Ndizoyenera kuyesa kufooka kwa maselo ofiira a m'magazi, kuwunika kwa mpweya wamagazi, kuyesa kwa hematocrit, kuchuluka kwa erythrocyte sedimentation ndi kutsimikiza kwachilengedwe chonse, koma osati. oyenera magazi coagulation mayeso.Kuchuluka kwa heparin kungayambitse kuphatikizika kwa maselo oyera amagazi ndipo sikungagwiritsidwe ntchito powerengera maselo oyera.Sikoyeneranso kugawika kwa leukocyte chifukwa imatha kupangitsa kuti filimu yamagazi ikhale yodetsedwa ndi buluu wowala.Itha kugwiritsidwa ntchito ngati rheology yamagazi.Mtundu wa chitsanzo ndi plasma.Mukangotenga magazi, tembenuzani ndikusakaniza kwa nthawi 5-8, ndikutenga madzi a m'magazi kuti mugwiritse ntchito.
7. Kapu yobiriwira yobiriwira ya chubu yolekanitsa plasma
Kuwonjezera heparin lithiamu anticoagulant kwa inert kulekana mphira chubu akhoza kukwaniritsa cholinga kulekana mofulumira plasma.Kwa mayesero adzidzidzi komanso ambiri a biochemical, monga chiwindi, ntchito ya impso, lipids ya magazi, shuga wa magazi, ndi zina zotero. Zitsanzo za Plasma zikhoza kuikidwa mwachindunji pamakina ndipo zimakhala zokhazikika kwa maola 48 pansi pa firiji.Itha kugwiritsidwa ntchito ngati rheology yamagazi.Mtundu wa chitsanzo ndi plasma.Mukangotenga magazi, tembenuzani ndikusakaniza kwa nthawi 5-8, ndikutenga madzi a m'magazi kuti mugwiritse ntchito.
8. Potaziyamu oxalate/sodium fluoride imvi kapu
Sodium fluoride ndi anticoagulant yofooka, yomwe nthawi zambiri imagwiritsidwa ntchito pamodzi ndi potassium oxalate kapena sodium ethiodate, ndipo chiŵerengero chake ndi 1 gawo la sodium fluoride ndi 3 magawo a potassium oxalate.4mg ya osakanizawa amatha kupanga 1ml ya magazi kuti asapangike mkati mwa masiku 23 ndikuletsa kuwonongeka kwa shuga.Sangagwiritsidwe ntchito kudziwa urea ndi urease njira, kapena kudziwa zamchere phosphatase ndi amylase.Ndikoyenera kuyesa shuga wamagazi.Muli ndi sodium fluoride kapena potassium oxalate kapena disodium ethylenediaminetetraacetate (EDTA-Na) spray, yomwe imatha kuletsa ntchito ya enolase mu metabolism ya glucose.Pambuyo kujambula magazi, inverted ndi kusakaniza kwa 5-8 zina.Madzi amadzimadzi a m'magazi amasungidwa kuti agwiritsidwe ntchito, ndipo ndi chubu chapadera choyezera shuga wamagazi mwachangu.
9. EDTA anticoagulation chubu wofiirira kapu
Ethylenediaminetetraacetic acid (EDTA, molekyulu yolemera 292) ndi mchere wake ndi amino polycarboxylic acid, woyenera kuyezetsa magazi wamba, ndipo ndi machubu oyesera omwe amawakonda pamayendedwe amagazi, hemoglobin ya glycosylated, ndi kuyesa kwamagulu amagazi.Osati abwino kwa coagulation mayeso ndi kupatsidwa zinthu za m`mwazi mayeso ntchito, kapena kutsimikiza kashiamu ion, potassium ion, sodium ion, ayoni chitsulo, zamchere phosphatase, creatine kinase ndi leucine aminopeptidase, oyenera PCR mayeso.Utsi 100ml wa 2.7% EDTA-K2 yankho pa khoma lamkati la vacuum chubu, youma pa 45 ° C, sonkhanitsani magazi mpaka 2ml, tembenuzani ndi kusakaniza ka 5-8 mutangotenga magazi, ndipo sakanizani bwino kuti mugwiritse ntchito mtsogolo.Mtundu wa chitsanzo ndi magazi athunthu, omwe amafunika kusakanizidwa mofanana musanagwiritse ntchito.
Nthawi yotumiza: Mar-02-2022
