Ka hoʻokaʻawale ʻana o ka vacuumnā kīʻaha koko
6. Heparin anticoagulation tube me ka pāpale ʻōmaʻomaʻo
Hoʻohui ʻia ʻo Heparin i ka paipu hōʻiliʻili koko.Loaʻa iā Heparin ka hopena o ka antithrombin, hiki ke hoʻolōʻihi i ka manawa coagulation o ka specimen.No ka pilikia a me ka nui o nā hoʻokolohua biochemical, e like me ka hana o ke akepaʻa, ka hana kīkī, nā lipids koko, ke kō koko, a me nā mea ʻē aʻe. He kūpono ia no ka hoʻāʻo ʻana i ka fragility o ke koko ʻulaʻula, ka nānā ʻana i ke kinoea koko, ka hoʻāʻo hematocrit, erythrocyte sedimentation rate a me ka hoʻoholo biochemical maʻamau, akā ʻaʻole. kūpono no ka hoʻāʻo ʻana i ke koko.Hiki i ka heparin nui ke hoʻohui i nā keʻokeʻo keʻokeʻo a ʻaʻole hiki ke hoʻohana ʻia no ka helu ʻana o ke koko keʻokeʻo.ʻAʻole kūpono ia no ka hoʻokaʻawale ʻana i ka leukocyte no ka mea hiki iā ia ke hana i ke kiʻiʻoniʻoni koko me ka ʻulaʻula uliuli.Hiki ke hoʻohana ʻia no ka rheology koko.ʻO ke ʻano hoʻohālike he plasma.Ma hope koke o ka hōʻiliʻili ʻana i ke koko, hoʻohuli a hui ʻia no nā manawa 5-8, a lawe i ka plasma luna no ka hoʻohana.
7. Kāpena ʻōmaʻomaʻo māmā o ka paipu hoʻokaʻawale plasma
ʻO ka hoʻohui ʻana i ka heparin lithium anticoagulant i ka inert separation rubber tube hiki ke hoʻokō i ke kumu o ka hoʻokaʻawale wikiwiki ʻana o ka plasma.No ka pilikia a me ka nui o nā hoʻokolohua biochemical, e like me ka hana o ka ate, ka hana kīkī, nā lipids koko, ke kō koko, a me nā mea ʻē aʻe. Hiki ke hoʻouka pololei ʻia nā laʻana Plasma ma ka mīkini a paʻa no nā hola 48 ma lalo o ka refrigeration.Hiki ke hoʻohana ʻia no ka rheology koko.ʻO ke ʻano hoʻohālike he plasma.Ma hope koke o ka hōʻiliʻili ʻana i ke koko, hoʻohuli a hui ʻia no nā manawa 5-8, a lawe i ka plasma luna no ka hoʻohana.
8. Potassium oxalate/sodium fluoride pāpale hina
ʻO ka sodium fluoride kahi anticoagulant nāwaliwali, i hoʻohana pinepine ʻia me ka potassium oxalate a i ʻole ka sodium ethiodate, a ʻo kāna ratio he 1 ʻāpana o ka sodium fluoride a me 3 mau ʻāpana o ka potassium oxalate.Hiki i ka 4mg o kēia hui ke hana i ka 1ml o ke koko ʻaʻole coagulate i loko o 23 mau lā a pale i ka decomposition o ke kō.ʻAʻole hiki ke hoʻohana ʻia no ka hoʻoholo ʻana i ka urea ma ke ʻano urease, ʻaʻole no ka hoʻoholo ʻana i ka alkaline phosphatase a me ka amylase.Manaʻo ʻia no ka hoʻāʻo ʻana i ke kō koko.Loaʻa iā ia ka sodium fluoride a i ʻole potassium oxalate a i ʻole disodium ethylenediaminetetraacetate (EDTA-Na) spray, hiki ke kāohi i ka hana enolase i ka metabolism glucose.Ma hope o ke kiʻi ʻana i ke koko, hoʻohuli a hui ʻia no 5-8 mau manawa.Mālama ʻia ka plasma wai no ka hoʻohana ʻana, a he paipu kūikawā no ke ana ʻana i ka glucose koko wikiwiki.
9. EDTA anticoagulation paipu poni pāpale
ʻO Ethylenediaminetetraacetic acid (EDTA, molecular weight 292) a me kāna mau paʻakai he amino polycarboxylic acid, kūpono no nā hoʻokolohua hematology maʻamau, a ʻo ia nā paipu hoʻāʻo i makemake ʻia no ke koko maʻamau, glycosylated hemoglobin, a me nā hoʻāʻo pūʻulu koko.ʻAʻole kūpono no ka hoʻāʻo coagulation a me ka hoʻāʻo ʻana o ka platelet function, ʻaʻole no ka hoʻoholo ʻana i ka calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase a me leucine aminopeptidase, kūpono no ka hoʻāʻo PCR.E kāpī i ka 100ml o 2.7% EDTA-K2 solution ma ka paia o loko o ka pahu hau, e puhi maloʻo ma 45 ° C, e hōʻiliʻili i ke koko i 2ml, e hoʻohuli a hui i nā manawa 5-8 ma hope koke o ke huki koko, a hui maikaʻi no ka hoʻohana hope.He koko holoʻokoʻa ke ʻano laʻana, pono e hui like ʻia ma mua o ka hoʻohana ʻana.
Ka manawa hoʻouna: Mar-02-2022
