Aia he 9 mau ʻano o ka vacuumpaipu ohi koko, i 'ike 'ia e ke kala o ka pāpale.
1. Ma'amau Serum Tube Ula Cap
ʻAʻohe mea hoʻohui i loko o ka pahu hōʻiliʻili koko, ʻaʻohe mea anticoagulant a i ʻole procoagulant, ʻo ka vacuum wale nō.Hoʻohana ʻia ia no ka biochemistry serum maʻamau, ka panakō koko a me nā hoʻokolohua pili i ka serology, nā ʻano hoʻokolohua biochemical a me ka immunological, e like me syphilis, ka helu hepa B, etc. ʻAʻole pono e hoʻoluliluli ʻia ma hope o ke kaha koko.ʻO ke ʻano o ka hoʻomākaukau specimen he serum.Ma hope o ka huki ʻana i ke koko, waiho ʻia i loko o kahi ʻauʻau wai 37 ° C no nā minuke 30, centrifuged, a hoʻohana ʻia ka serum luna no ka hoʻohana hope.
2. Paʻi ʻalani Serum wikiwiki
Aia ka coagulant i loko o ka paipu hōʻiliʻili koko e wikiwiki i ke kaʻina coagulation.Hiki i ka paipu serum wikiwiki ke coagulate i ke koko i hōʻiliʻili ʻia i loko o 5 mau minuke.He kūpono ia no nā hōʻike hoʻāʻo serum ulia pōpilikia.ʻO ia ka pahu hoʻāʻo coagulation i hoʻohana pinepine ʻia no kēlā me kēia lā biochemistry, immunity, serum, hormones, etc. Ma hope o ke huki ʻana o ke koko, hoʻohuli a hoʻohui i nā manawa 5-8.Ke haʻahaʻa ka mahana, hiki ke waiho ʻia i loko o kahi ʻauʻau wai 37 ° C no 10-20min, a hiki ke centrifuged ka serum luna no ka hoʻohana hope.
3. Ka pāpale gula o ka inert separation gel accelerator tube
Hoʻohui ʻia ka gel hoʻokaʻawale inert a me ka coagulant i ka paipu hōʻiliʻili koko.Paʻa nā mea hoʻohālike no 48 mau hola ma hope o ka centrifugation.Hiki i nā procoagulants ke hoʻoikaika koke i ka mīkini coagulation a hoʻoikaika i ke kaʻina hana coagulation.ʻO ke ʻano o ka specimen i hoʻomākaukau ʻia he serum, kūpono ia no nā hoʻokolohua serum biochemical a me ka pharmacokinetic.Ma hope o ka hōʻiliʻili ʻana, hoʻohuli a hui pū i nā manawa 5-8, kū pololei no 20-30 min, a centrifuge i ka supernatant no ka hoʻohana ʻana ma hope.
4. Sodium citrate ESR pahu ho'āʻo ʻeleʻele pāpale
He 3.2% ka nui o ka sodium citrate no ka ho'āʻo ESR (e like me 0.109mol/L), a ʻo ka ratio o ka anticoagulant i ke koko he 1:4.Loaʻa iā 0.4 mL o 3.8% sodium citrate, a huki i ke koko i 2.0 mL.He paipu ho'āʻo kūikawā kēia no ka helu erythrocyte sedimentation rate.ʻO ke ʻano laʻana he plasma, kahi kūpono no ka helu sedimentation erythrocyte.Ma hope koke o ke huki koko, hoʻohuli a hui i nā manawa 5-8.E lulu maikaʻi ma mua o ka hoʻohana ʻana.ʻO ka ʻokoʻa ma waena o ia a me ka paipu hōʻike no ka hoʻāʻo ʻana i ka coagulation factor ka ʻokoʻa ma waena o ka neʻe ʻana o ka anticoagulant a me ka ratio o ke koko, ʻaʻole pono e huikau.
5. ʻO ka pahu hoʻāʻo ʻo Sodium citrate coagulation hoʻāʻo ʻulaʻula polū
Hana nui ka sodium citrate ma ke ʻano he anticoagulant ma o ka hoʻokaʻawale ʻana i nā ion calcium i loko o nā laʻana koko.ʻO ka manaʻo anticoagulant i ʻōlelo ʻia e ka National Committee for Standardization of Clinical Laboratories he 3.2% a i ʻole 3.8% (e like me 0.109mol/L a i ʻole 0.129mol/L), a ʻo ka ratio o ka anticoagulant i ke koko he 1:9.Aia ma kahi o 0.2 mL o ka 3.2% sodium citrate anticoagulant i loko o ka pahu ʻohi koko, a ʻohi ʻia ke koko i 2.0 mL.ʻO ke ʻano hoʻomākaukau laʻana he koko holoʻokoʻa a i ʻole plasma.Ma hope koke o ka hōʻiliʻili ʻana, hoʻohuli a hoʻohui i nā manawa 5-8.Ma hope o ka centrifugation, e lawe i ka plasma luna no ka hoʻohana.He kūpono no nā hoʻokolohua coagulation, PT, APTT, hōʻike kumu coagulation.
Ka manawa hoʻouna: Feb-28-2022
