Te whakarōpūtanga me te whakaahuatanga o nga ngongo kohinga toto korehau
1. Ko te ngongo serum noa he potae whero, a kaore he taapiri o te ngongo kohinga toto.Ka whakamahia mo te koiora serum biochemistry, peeke toto me nga whakamatautau e pa ana ki te serology.
2. Ko te potae karaka-whero o te ngongo serum tere he coagulant i roto i te ngongo kohinga toto hei whakatere i te mahi whakakoi.Ka taea e te ngongo serum tere te whakakotahi i te toto kua kohia i roto i te 5 meneti, he pai mo te whakamatautau serum ohorere.
3. Ko te potae koura o te ngongo whakakoi ira whakawehe inert, me te reera whakawehe kore me te coagulant ka taapirihia ki te ngongo kohinga toto.I muri i te centrifuged o te tauira, ka taea e te kaera wehenga inert te wehe i nga waahanga wai (serum, plasma ranei) me nga waahanga totoka (nga toto toto whero, nga toto toto ma, platelets, fibrin, me etahi atu) i roto i te toto ka whakaemi katoa ki te pokapū o te ipuipu hei hanga arai.Ko te tauira kei roto i te 48 haora kia u tonu.Ka taea e te coagulant te tere te whakahohe i te tikanga coagulation me te whakatere i te tukanga coagulation, e tika ana mo nga whakamatautau biochemical serum ohorere.
4. Ko te potae matomato o te ngongo anticoagulation heparin, me te heparin kua whakauruhia ki roto i te ngongo kohinga toto.Ko te Heparin tika te paanga o te antithrombin, ka taea te whakaroa i te wa kato o te tauira.He pai mo te whakamatautau ngoikore o te toto toto whero, te tātari hau toto, te whakamatautau hematocrit, te tere parataiao erythrocyte me te whakatau i te kaha o te koiora, kaore e pai mo te whakamatautau toto.Ko te nui o te heparin ka taea te whakaemi o nga rerenga toto ma, kaore e taea te whakamahi mo te tatau toto toto ma.Kaore e pai mo te whakarōpūtanga o nga pūtau toto ma na te mea ka pokea te poro toto ki te papamuri kikorangi kikorangi.
5. Ko te uhi o te upoko matomato marama o te ngongo wehenga plasma, te whakauru i te lithium heparin anticoagulant ki roto i te ngongo wehenga inert, ka taea te whakatutuki i te kaupapa o te wehenga plasma tere, ko te mea pai rawa atu mo te kitenga electrolyte, a ka taea hoki te whakamahi mo nga mahi koiora plasma. whakatau me
ICU me etahi atu whakamatautau koiora plasma ohorere.Ka taea te whakanoho tika i nga tauira plasma ki runga i te miihini ka noho pumau mo te 48 haora i raro i te pouaka whakamatao.
6. EDTA anticoagulation ngongo potae papura, te waikawa ethylenediaminetetraacetic (EDTA, te taumaha molecular 292) me tona tote he waikawa polycarboxylic amino, ka taea te tarai i nga katote konupūmā i roto i nga tauira toto, te konupūmā chelate, te tauhohe ranei i te konupūmā Ko te tangohanga o te pae ka aukati, ka mutu Ko te tukanga o te whakaheke toto, o waho ranei, na reira ka kore e pakaru te tauira toto.He pai mo te whakamatautau hematology whanui,
Kaore e pai mo te whakamatautau toto toto me te whakamatautau mahi platelet, mo te whakatau i te katote konupūmā, te katote pāhare pāporo, te katote konutai, te katote rino, te phosphatase alkaline, te creatine kinase me te leucine aminopeptidase me te whakamatautau PCR.
7. He potae kikorangi marama te ngongo whakamatautau konutai citrate coagulation.Ko te konutai citrate te nuinga e whakamahia ana mo te whakamomori ma te chelating me nga katote konupūmā i roto i te tauira toto.He pai mo nga whakamatautau toto toto.Ko te kukū o te anticoagulant e taunakihia ana e te Komiti Paerewa o te National Clinical Laboratory he
3.2% ranei 3.8% (he rite ki te 0.109mol/L, 0.129mol/L ranei), ko te tauwehenga o te anticoagulant ki te toto he 1:9.
8. Ko te konutai citrate erythrocyte sedimentation rate ngongo whakamatautau, te uhi mahunga pango, te kukū konutai citrate e hiahiatia ana mo te whakamatautau tere o te erythrocyte sedimentation he 3.2% (he rite ki te 0.109mo/u), ko te tauwehenga o te anticoagulant ki te toto he 1:4.
Te pāhare pāporo oxalate/konutai pūkōwhai hei uhi māhunga hina.Ko te konutai fluoride he anticoagulant ngoikore.Ko te tikanga, ko te konutai oxalate me te konutai diiodate ka whakamahia tahi.Ko te ōwehenga he 1 wāhanga o te konutai pūkōwhai me te 3 wāhanga o te pāhare pāporo oxalate.Ka taea e te 4mg o tenei ranunga te aukati i te 1m o te toto mai i te whakakotahi me te aukati i te pirau huka i roto i nga ra 23.He rongoa pai mo te whakatau i te huka toto.Kaore e taea te whakamahi mo te whakatau i te urea ma te tikanga urease, kaore hoki e whakamahia mo te whakatau i te alkaline phosphatase me te amylase.E taunaki ana mo te whakamatautau toto huka.
Wā tuku: Sep-18-2021
