Classification and description of vacuum blood collection tubes
1. Common serum tube has a red cap, and the blood collection tube does not contain additives. It is used for routine serum biochemistry, blood bank and serology related tests.
2. The orange-red cap of the fast serum tube has a coagulant in the blood collection tube to accelerate the coagulation process. The rapid serum tube can coagulate the collected blood within 5 minutes, which is suitable for serialized emergency serum test.
3. Golden cap of the inert separating gel coagulation tube, and inert separating gel and coagulant are added in the blood collection tube. After the specimen is centrifuged, the inert separation gel can completely separate the liquid components (serum or plasma) and solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood and completely accumulate in the center of the test tube to form a barrier. The specimen is within 48 hours keep it steady. The coagulant can quickly activate the coagulation mechanism and accelerate the coagulation process, which is suitable for emergency serum biochemical tests.
4. The green cap of the heparin anticoagulation tube, with heparin added in the blood collection tube. Heparin directly has the effect of antithrombin, which can prolong the clotting time of the specimen. It is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general energy biochemical determination, not suitable for blood coagulation test. Excessive heparin can cause the accumulation of white blood cells and cannot be used for white blood cell counting. It is not suitable for white blood cell classification because it can stain the blood slice with a light blue background.
5. The light green head cover of the plasma separation tube, adding lithium heparin anticoagulant into the inert separation hose, can achieve the purpose of rapid plasma separation, it is the best choice for electrolyte detection, and can also be used for routine plasma biochemical determination and
ICU and other emergency plasma biochemical tests. Plasma samples can be directly put on the machine and kept stable for 48 hours under refrigeration.
6. EDTA anticoagulation tube purple cap, ethylenediaminetetraacetic acid (EDTA, molecular weight 292) and its salt are an amino polycarboxylic acid, which can effectively chelate calcium ions in blood samples, chelate calcium or react calcium The removal of the site will block and terminate the endogenous or exogenous coagulation process, thereby preventing the blood specimen from clotting. Suitable for general hematology test,
It is not suitable for blood coagulation test and platelet function test, nor for determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test.
7. The sodium citrate coagulation test tube has a light blue cap. Sodium citrate is mainly used for anticoagulation by chelating with calcium ions in the blood sample. It is suitable for blood coagulation experiments. The concentration of anticoagulant recommended by the Standardization Committee of the National Clinical Laboratory is
3.2% or 3.8% (equivalent to 0.109mol/L or 0.129mol/L), the ratio of anticoagulant to blood is 1:9.
8. Sodium citrate erythrocyte sedimentation rate test tube, black head cover, the sodium citrate concentration required for erythrocyte sedimentation rate test is 3.2% (equivalent to 0.109mo/u), the ratio of anticoagulant to blood is 1:4.
Potassium oxalate/sodium fluoride gray head cover. Sodium fluoride is a weak anticoagulant. Generally, potassium oxalate or sodium diiodate are used in combination. The ratio is 1 part of sodium fluoride and 3 parts of potassium oxalate. 4mg of this mixture can prevent 1m of blood from coagulating and inhibit sugar decomposition within 23 days. It is a good preservative for blood glucose determination. It cannot be used for the determination of urea by the urease method, nor is it used for the determination of alkaline phosphatase and amylase. Recommended for blood glucose testing.
Post time: Sep-18-2021
