Ukuhlukaniswa kanye nencazelo yamashubhu okuqoqa igazi evacuum
1. Ishubhu ye-serum evamile inekepisi elibomvu, futhi ishubhu lokuqoqa igazi alinazo izithasiselo.Isetshenziselwa ukuhlolwa kwe-serum biochemistry, ibhange legazi kanye nokuhlolwa okuhlobene ne-serology.
2. Ikepisi elibomvu ngokusawolintshi leshubhu le-serum esheshayo line-coagulant eshubhu lokuqoqa igazi ukusheshisa inqubo yokujiya.Ishubhu le-serum elisheshayo lingakwazi ukubopha igazi eliqoqiwe phakathi nemizuzu emi-5, elilungele ukuhlolwa kwe-serum esiphuthumayo.
3. Isigqoko segolide se-inert ehlukanisa i-gel coagulation tube, kanye nejeli ehlukanisayo engenzi lutho kanye ne-coagulant yengezwe eshubhu lokuqoqa igazi.Ngemuva kokuthi isampula senziwe i-centrifuged, ijeli yokuhlukanisa i-inert ingakwazi ukuhlukanisa ngokuphelele izingxenye eziwuketshezi (i-serum noma i-plasma) kanye nezingxenye eziqinile (amangqamuzana egazi abomvu, amangqamuzana egazi amhlophe, amaplatelet, i-fibrin, njll.) egazini futhi aqoqeke ngokuphelele phakathi nendawo. ithubhu lokuhlola ukwenza umgoqo.Isifanekiso siphakathi kwamahora angu-48 sigcine singanyakazi.I-coagulant ingakwazi ukwenza kusebenze umshini wokuhlangana futhi isheshise inqubo yokuhlangana, efanele ukuhlolwa okuphuthumayo kwe-serum biochemical.
4. Ikepisi eliluhlaza leshubhu le-heparin anticoagulation, eline-heparin yengezwe eshubhu lokuqoqa igazi.I-Heparin ngokuqondile inomphumela we-antithrombin, engandisa isikhathi sokujiya kwesifanekiso.Ifanele ukuhlolwa kokuqina kwengqamuzana elibomvu legazi, ukuhlaziywa kwegesi yegazi, ukuhlolwa kwe-hematocrit, izinga le-erythrocyte sedimentation kanye nokunqunywa kwamakhemikhali e-biochemical amandla jikelele, ayifanele ukuhlolwa kwe-blood coagulation.I-heparin eyeqile ingabangela ukunqwabelana kwamangqamuzana amhlophe egazi futhi ayikwazi ukusetshenziselwa ukubala amangqamuzana amhlophe egazi.Ayilungele ukuhlukaniswa kwamaseli amhlophe egazi ngoba ingangcolisa ucezu lwegazi ngengemuva eliluhlaza okwesibhakabhaka.
5. Isembozo sekhanda esiluhlaza esikhanyayo seshubhu lokuhlukanisa i-plasma, sinezela i-lithium heparin anticoagulant epayipini lokuhlukanisa le-inert, singafinyelela inhloso yokuhlukaniswa kwe-plasma ngokushesha, iyisinqumo esingcono kakhulu sokutholwa kwe-electrolyte, futhi ingasetshenziselwa i-plasma biochemical ejwayelekile. ukuzimisela kanye
I-ICU nezinye izivivinyo eziphuthumayo ze-plasma biochemical.Amasampula e-plasma angafakwa ngokuqondile emshinini futhi agcinwe ezinzile amahora angama-48 ngaphansi kwesiqandisi.
6. I-EDTA anticoagulation tube purple cap, ethylenediaminetetraacetic acid (EDTA, i-molecular weight 292) kanye nosawoti wayo i-amino polycarboxylic acid, engakwazi ukuhlanza kahle i-calcium ions kumasampula egazi, i-chelate calcium noma i-react calcium Ukususwa kwesayithi kuzovimba futhi kuqedwe. inqubo ye-endogenous noma exogenous coagulation, ngaleyo ndlela ivimbele isifanekiso segazi ekuhlulekeni.Ifanele ukuhlolwa kwe-hematology evamile,
Ayilungele ukuhlolwa kwe-blood coagulation kanye nokuhlolwa kokusebenza kweplatelet, noma ukunqunywa kwe-calcium ion, i-potassium ion, i-sodium ion, i-iron ion, i-alkaline phosphatase, i-creatine kinase ne-leucine aminopeptidase kanye nokuhlolwa kwe-PCR.
7. Ishubhu ye-sodium citrate coagulation test inekepisi eliluhlaza okwesibhakabhaka.I-sodium citrate isetshenziselwa kakhulu i-anticoagulation nge-chelating ne-calcium ions kusampula yegazi.Ilungele ukuhlolwa kokuqina kwegazi.Ukuhlushwa kwe-anticoagulant okunconywe yiKomidi Lokulinganisa le-National Clinical Laboratory ngu
3.2% noma 3.8% (okulingana no-0.109mol/L noma 0.129mol/L), isilinganiso se-anticoagulant negazi singu-1:9.
8. I-sodium citrate erythrocyte sedimentation rate rate tube test, ikhava yekhanda elimnyama, i-sodium citrate concentration edingekayo ukuze kuhlolwe izinga le-erythrocyte sedimentation rate ngu-3.2% (okulingana no-0.109mo/u), isilinganiso se-anticoagulant negazi ngu-1:4.
Ikhava yekhanda elimpunga nge-potassium oxalate/sodium fluoride.I-sodium fluoride iyi-anticoagulant ebuthakathaka.Ngokuvamile, i-potassium oxalate noma i-sodium diiodate isetshenziswa ngokuhlanganiswa.Isilinganiso siyingxenye engu-1 ye-sodium fluoride kanye nezingxenye ezi-3 ze-potassium oxalate.I-4mg yale ngxube ingavimbela i-1m yegazi ukuthi ingahlangani futhi ivimbele ukubola koshukela phakathi kwezinsuku ezingu-23.Kuyisilondolozi esihle sokunqunywa kweglucose yegazi.Ayikwazi ukusetshenziselwa ukunqunywa kwe-urea ngendlela ye-urease, futhi ayisetshenziselwa ukunquma i-alkaline phosphatase ne-amylase.Inconyelwe ukuhlolwa kweglucose yegazi.
Isikhathi sokuthumela: Sep-18-2021
