Te whakarōpūtanga me te whakaahuatanga ongongo kohinga toto
1. Ngongo serum noa me te potae whero, ngongo kohinga toto kaore he taapiri, ka whakamahia mo nga mahi koiora serum, peeke toto me nga whakamatautau e pa ana ki te serology.
2. Ko te uhi mahunga karaka-whero o te ngongo serum tere he coagulant kei roto i te ngongo kohinga toto hei whakatere i te mahi whakakoi.Ka taea e te ngongo serum tere te whakakotahi i te toto kua kohia i roto i te 5 meneti, he pai hoki mo nga whakamatautau rangatū serum ohorere.
3. Ko te potae koura o te ngongo whakakorikori inert separation gel accelerator, me te reera wehenga inert me te coagulant ka taapiri atu ki te ngongo kohinga toto.I muri i te centrifuged o te tauira, ka taea e te kaera whakawehe inert te wehe i nga waahanga wai (serum, plasma ranei) me nga waahanga totoka (nga toto toto whero, nga toto toto ma, platelets, fibrin, me etahi atu) i roto i te toto ka whakaemi katoa ki te pokapū o te ipuipu hei hanga parenga.kia mau tonu.Ka taea e nga Procoagulants te tere te whakahohe i te tikanga whakakoi me te whakatere i te tukanga o te coagulation, he pai hoki mo nga whakamatautau koiora serum ohorere.
4. Ko te ngongo anticoagulation heparin he potae matomato, ka whakauruhia he heparin ki te ngongo kohinga toto.Ko te Heparin tika te paanga o te antithrombin, ka taea te whakaroa i te wa o te coagulation o te tauira.He pai mo te whakamatautau ngoikore o te toto toto whero, te tātari hau toto, te whakamatautau hematocrit, te tere parataiao erythrocyte me te whakatau i te kaha o te koiora, engari kaore e pai mo te whakamatautau toto.Ko te nui o te heparin ka taea te whakahiato o nga pūtau toto ma, kaore e taea te whakamahi mo nga tatau toto toto ma.Kaore hoki i te pai mo te whakarōpū leukocyte na te mea ka pokea te kiriata toto ki te papamuri kahurangi marama.
5. Ko te uhi mahunga matomato marama o te ngongo wehenga plasma, te taapiri i te heparin lithium anticoagulant ki te ngongo rapa wehenga inert, ka taea te whakatutuki i te kaupapa o te wehenga tere o te plasma, ko te mea pai rawa atu mo te kitenga electrolyte, ka taea hoki te whakamahi mo nga mahinga. te inenga koiora plasma me te plasma ohorere penei i te whakamatautau ICU Biochemical.Ka taea te utaina nga tauira plasma ki runga i te miihini, ka noho pumau mo nga haora 48 i raro i te whakamatao.
6. EDTA anticoagulation ngongo potae papura, te waikawa ethylenediaminetetraacetic (EDTA, te taumaha molecular 292) me ona tote he waikawa polycarboxylic amino, ka taea te tarai i nga katote konupūmā i roto i nga tauira toto, te konupūmā chelate, te tauhohe ranei i te konupūmā.Ko te tango i te waahi ka aukati, ka whakamutua hoki te tukanga o te whakakoi o waho, o waho ranei, na reira ka kore te tauira toto e paheke.He pai mo nga whakamatautau hematological whanui, kaore e pai mo te whakamatautau coagulation me te whakamatautau mahi platelet, mo te whakatau i te katote konupūmā, te katote konutai, te katote konutai, te rino katote, te alkaline phosphatase, te creatine kinase me te leucine aminopeptidase me te whakamatautau PCR.
7. He potae kikorangi marama te ngongo whakamatautau konutai citrate coagulation.Ko te konutai citrate te whai hua anticoagulant i te nuinga o te waa ma te tarai i nga katote konupūmā i roto i te tauira toto.E pa ana ki nga whakamatautau whakamohoatanga, ko te kukū anticoagulant e taunakihia ana e te Komiti Paerewa o te National Provisional Laboratory he 3.2%, 3.8% ranei (he rite ki te 0.109mol / L ranei 0.129mol / L), me te tauwehenga o te anticoagulant ki te toto he 1:9.
8. Sodium citrate erythrocyte sedimentation rate test tube Te potae pango, ko te konutai konutai citrate e hiahiatia ana mo te whakamatautau tere o te erythrocyte sedimentation he 3.2% (he rite ki te 0.109mol / L), me te tauwehenga o te anticoagulant ki te toto he 1:4.
9. Pāhare pāporo oxalate/konutai pūkōwhai pōtae hina, konutai pūkōwhai he anticoagulant ngoikore, te tikanga ngā ki pāhare pāporo oxalate konutai iodate ranei, te ōwehenga ko te 1 wahi o te konutai pūkōwhai, 3 wāhanga o te pāhare pāporo oxalate .Ko te 4mg o tenei ranunga ka taea te 1ml o te toto kia kore e whakakotahi me te aukati i te glycolysis i roto i nga ra 23.He rongoa pai mo te whakatau i te huka toto, kaore e taea te whakamahi mo te whakatau i te urea ma te tikanga urease, mo te whakatau i te alkaline phosphatase me te amylase.E taunaki ana mo te whakamatautau huka toto.
Wā tuku: Maehe-07-2022
