Ukuhlukaniswa kanye nencazelo yeamashubhu okuqoqa igazi
1. Ishubhu le-serum elivamile elinekepisi elibomvu, ishubhu lokuqoqa igazi ngaphandle kwezithasiselo, elisetshenziselwa isimiso se-serum biochemistry, ibhange legazi kanye nokuhlolwa okuhlobene ne-serology.
2. Isembozo sekhanda esibomvu-owolintshi seshubhu le-serum esheshayo sine-coagulant eshubhu lokuqoqa igazi ukusheshisa inqubo yokuhlangana.Ishubhu le-serum elisheshayo lingakwazi ukubopha igazi eliqoqiwe phakathi nemizuzu emi-5, futhi lilungele ukuhlolwa kwe-serum okuphuthumayo.
3. Isigqoko segolide seshubhu ye-accelerator yokuhlukanisa i-inert, kanye nejeli yokuhlukanisa i-inert kanye ne-coagulant yengezwe ku-tube yokuqoqa igazi.Ngemuva kokuthi isampula senziwe i-centrifuged, ijeli yokuhlukanisa i-inert ingahlukanisa ngokuphelele izingxenye eziwuketshezi (i-serum noma i-plasma) kanye nezingxenye eziqinile (amangqamuzana egazi abomvu, amangqamuzana egazi amhlophe, ama-platelet, i-fibrin, njll.) egazini futhi aqoqwe ngokuphelele phakathi nendawo. ithubhu lokuhlola ukwenza umgoqo.kugcine kuqinile.Ama-procoagulant angakwazi ukwenza kusebenze indlela yokuhlangana ngokushesha futhi asheshise inqubo yokuhlangana, futhi alungele ukuhlolwa okuphuthumayo kwe-serum biochemical.
4. Ishubhu le-heparin anticoagulation linekepisi eliluhlaza, futhi i-heparin yengezwa eshubhu lokuqoqa igazi.I-Heparin ngokuqondile inomphumela we-antithrombin, engandisa isikhathi sokuhlangana kwesampula.Ifanele ukuhlolwa kokuqina kwamangqamuzana egazi abomvu, ukuhlaziya igesi yegazi, ukuhlolwa kwe-hematocrit, izinga le-erythrocyte sedimentation kanye nokunqunywa kwe-biochemical yamandla okuvamile, kodwa ayifanele ukuhlolwa kwe-blood coagulation.I-heparin eyeqile ingabangela ukuhlangana kwamangqamuzana amhlophe egazi futhi ayikwazi ukusetshenziselwa ukubala amangqamuzana amhlophe egazi.Ayifanele futhi ukuhlukaniswa kwe-leukocyte ngoba ingenza ifilimu yegazi ingcoliswe ngengemuva eliluhlaza okwesibhakabhaka.
5. Isembozo sekhanda esiluhlaza esikhanyayo seshubhu lokuhlukanisa i-plasma, enezela i-heparin lithium anticoagulant kushubhu yerabha yokuhlukanisa engasebenzi, ingafinyelela injongo yokuhlukaniswa ngokushesha kwe-plasma, okuyisinqumo esingcono kakhulu sokutholwa kwe-electrolyte, futhi ingasetshenziselwa isimiso i-plasma biochemical measurement kanye ne-plasma ephuthumayo njengokuhlolwa kwe-ICU Biochemical.Amasampula e-plasma angalayishwa ngqo emshinini futhi azinzile amahora angama-48 ngaphansi kwesiqandisi.
6. I-EDTA i-anticoagulation tube cap purple, i-ethylenediaminetetraacetic acid (EDTA, isisindo se-molecular 292) kanye nosawoti wayo i-amino polycarboxylic acid, engakwazi ukuhlukanisa ngokuphumelelayo i-calcium ions kumasampula egazi, i-chelate calcium noma i-react calcium.Ukususwa kwesayithi kuzovimba futhi kunqamule inqubo ye-endogenous noma exogenous coagulation, ngaleyo ndlela kuvimbele isampula yegazi ukuthi ingahlangani.Ilungele ukuhlolwa kwe-hematological okuvamile, ayilungele ukuhlolwa kwe-coagulation kanye nokuhlolwa kokusebenza kweplatelet, noma ukunqunywa kwe-calcium ion, i-potassium ion, i-sodium ion, i-iron ion, i-alkaline phosphatase, i-creatine kinase ne-leucine aminopeptidase kanye nokuhlolwa kwe-PCR.
7. Ishubhu ye-sodium citrate coagulation test inekepisi eliluhlaza okwesibhakabhaka.I-sodium citrate idlala umphumela we-anticoagulant ikakhulukazi ngokuchela ama-ion e-calcium kusampula yegazi.Isebenza ekuhlolweni kokujiya, ukugxilwa kwe-anticoagulant okunconywe iKomidi Lokulinganisa Le-National Provisional Laboratory ngu-3.2% noma u-3.8% (okulingana no-0.109mol/L noma 0.129mol/L), futhi isilinganiso se-anticoagulant negazi singu-1:9.
8. I-sodium citrate erythrocyte sedimentation rate rate tube test I-cap Black, i-sodium citrate concentration edingekayo ukuze kuhlolwe izinga le-erythrocyte sedimentation rate ngu-3.2% (okulingana no-0.109mol/L), kanti isilinganiso se-anticoagulant negazi singu-1:4.
9. I-Potassium oxalate/sodium fluoride grey cap, i-sodium fluoride iyi-anticoagulant ebuthakathaka, ngokuvamile ihlanganiswe ne-potassium oxalate noma i-sodium iodate, isilinganiso siyingxenye engu-1 ye-sodium fluoride, izingxenye ezi-3 ze-potassium oxalate.I-4mg yale ngxube ingenza i-1ml yegazi ingajiki futhi ivimbele i-glycolysis phakathi kwezinsuku ezingu-23.Kuyisilondolozi esihle sokunqunywa kweglucose yegazi, futhi ayikwazi ukusetshenziselwa ukunqunywa kwe-urea ngendlela ye-urease, noma ekunqumeni i-alkaline phosphatase ne-amylase.Inconyelwe ukuhlola ushukela wegazi.
Isikhathi sokuthumela: Mar-07-2022
