Gulu ndi kufotokozeramachubu osonkhanitsira magazi
1. Machubu a seramu wamba okhala ndi kapu yofiyira, chubu chosonkhanitsira magazi popanda zowonjezera, zomwe zimagwiritsidwa ntchito ngati seramu biochemistry, banki yamagazi ndi mayeso okhudzana ndi serology.
2. Chivundikiro chamutu chofiira chalalanje cha chubu chofulumira cha seramu chimakhala ndi coagulant mu chubu chosonkhanitsira magazi kuti ifulumizitse njira ya coagulation.Chubu chofulumira cha seramu chimatha kulumikiza magazi osonkhanitsidwa mkati mwa mphindi 5, ndipo ndi oyenera kuyezetsa mwadzidzidzi seramu.
3. Chipewa chagolide cha chubu chothamangitsira gel olekanitsa inert, ndi gel olekanitsa inert ndi coagulant zimawonjezedwa ku chubu chotolera magazi.Pambuyo chitsanzo ndi centrifuged, inert kulekana gel osakaniza angathe kulekanitsa kwathunthu madzi zigawo zikuluzikulu (seramu kapena madzi a m'magazi) ndi olimba zigawo zikuluzikulu (maselo ofiira a magazi, maselo oyera a magazi, mapulateleti, fibrin, etc.) m'magazi ndipo kwathunthu kudziunjikira pakati pa chubu choyesera kupanga chotchinga.sungani mokhazikika.Ma procoagulants amatha kuyambitsa makina a coagulation mwachangu ndikufulumizitsa njira ya coagulation, ndipo ndi oyenera kuyesa kwadzidzidzi kwa seramu biochemical.
4. The heparin anticoagulation chubu imakhala ndi kapu yobiriwira, ndipo heparin imawonjezeredwa ku chubu chosonkhanitsa magazi.Heparin mwachindunji imakhala ndi zotsatira za antithrombin, zomwe zimatha kutalikitsa nthawi ya coagulation ya chitsanzocho.Ndizoyenera kuyesa kufooka kwa maselo ofiira a m'magazi, kusanthula kwa mpweya wamagazi, kuyesa kwa hematocrit, kuchuluka kwa erythrocyte sedimentation ndi kutsimikiza kwamphamvu kwamankhwala am'magazi, koma sikoyenera kuyesa magazi a coagulation.Kuchuluka kwa heparin kungayambitse kuphatikizika kwa maselo oyera amagazi ndipo sikungagwiritsidwe ntchito powerengera maselo oyera.Sikoyeneranso kugawika kwa leukocyte chifukwa imatha kupangitsa kuti filimu yamagazi ikhale yodetsedwa ndi buluu wowala.
5. Chivundikiro chamutu chobiriwira chobiriwira cha chubu cholekanitsa cha plasma, kuwonjezera heparin lithiamu anticoagulant ku chubu cha rabara cha inert, chingathe kukwaniritsa cholinga cha kulekanitsa mofulumira kwa plasma, yomwe ndi yabwino kwambiri pozindikira electrolyte, ndipo ingagwiritsidwe ntchito chizolowezi. plasma biochemical muyeso ndi plasma mwadzidzidzi monga ICU Biochemical kuyesa.Zitsanzo za plasma zimatha kuyikidwa mwachindunji pamakina ndipo zimakhala zokhazikika kwa maola 48 pansi pafiriji.
6. EDTA anticoagulation chubu chofiirira kapu, ethylenediaminetetraacetic acid (EDTA, molekyulu kulemera 292) ndi mchere wake ndi amino polycarboxylic acid, amene angathe bwino chelate calcium ions mu zitsanzo za magazi, chelate calcium kapena kuchitapo kanthu calcium.Kuchotsa kwa malo kudzatsekereza ndikuthetsa njira ya endogenous kapena exogenous coagulation, potero kulepheretsa kuti magazi asamapangidwe.Ndi oyenera ambiri hematological mayesero, si oyenera coagulation mayeso ndi kupatsidwa zinthu za m`mwazi mayeso ntchito, kapena kutsimikiza kashiamu ion, potaziyamu ion, sodium ion, ayoni chitsulo, zamchere phosphatase, creatine kinase ndi leucine aminopeptidase ndi PCR mayeso.
7. Sodium citrate coagulation test chubu ili ndi kapu yabuluu yowala.Sodium citrate imakhala ndi anticoagulant kwenikweni ndi chelating ayoni a calcium m'magazi.Kugwiritsidwa ntchito pakuyesa kwa coagulation, ndende ya anticoagulant yomwe ikulimbikitsidwa ndi Standardization Committee ya National Provisional Laboratory ndi 3.2% kapena 3.8% (yofanana ndi 0.109mol/L kapena 0.129mol/L), ndipo chiŵerengero cha anticoagulant ndi magazi ndi 1:9.
8. Sodium citrate erythrocyte sedimentation rate test chubu Black cap, sodium citrate concentration yofunikira pa kuyesa kwa erythrocyte sedimentation rate ndi 3.2% (yofanana ndi 0.109mol / L), ndipo chiŵerengero cha anticoagulant ndi magazi ndi 1: 4.
9. Potaziyamu oxalate / sodium fluoride imvi kapu, sodium fluoride ndi ofooka anticoagulant, kawirikawiri pamodzi potassium oxalate kapena sodium iodate, chiŵerengero ndi 1 gawo la sodium fluoride, 3 mbali potassium oxalate.4mg ya kusakaniza kumeneku kungapangitse 1ml ya magazi kuti isagwirizane ndikuletsa glycolysis mkati mwa masiku 23.Ndiwoteteza bwino kutsimikiza kwa shuga m'magazi, ndipo sangagwiritsidwe ntchito pozindikira urea ndi njira ya urease, kapenanso kudziwa zamchere phosphatase ndi amylase.Akulimbikitsidwa kuyezetsa shuga wamagazi.
Nthawi yotumiza: Mar-07-2022
