Rarraba da bayanintarin jini
1. Biochemical
An kasu bututun tattara jini na biochemical zuwa bututu marasa kyauta (jan hula), bututu masu haɓaka coagulation (orange-ja hula), da bututun roba na rabuwa ( hular rawaya).
Bangon ciki na bututun tarin jini mara inganci mai inganci yana da kyau sosai tare da wakili na jiyya na bango na ciki da wakili na maganin bututu don guje wa karyewar kwayar halitta yayin centrifugation kuma yana shafar sakamakon gwajin, kuma bangon ciki na bututu da magani a bayyane yake. kuma a bayyane, kuma babu jini da ke rataye a bakin bututun.
Baya ga bangon ciki na bututun coagulation ana lullube shi tare da wakilin jiyya na bango na ciki da kuma wakilin jiyya na bututun ƙarfe, ana amfani da hanyar fesa a cikin bututu don sanya mai haɓakawar coagulation daidai da bangon bututu, wanda ya dace da sauri. da kuma hada samfurin jini gaba daya bayan an yi samfur, wanda zai iya rage yawan lokacin coagulation.Kuma babu hazo na fibrin filaments don gujewa toshe ramin kayan aiki yayin yin samfur.
Lokacin da keɓaɓɓen bututun roba ya kasance centrifuged, an motsa gel ɗin rabuwa zuwa tsakiyar bututu, wanda ke tsakanin ruwan magani ko plasma da abubuwan da aka samar da jini.Bayan an kammala centrifugation, yana ƙarfafa don samar da shinge, wanda ke raba ruwan jini ko plasma gaba ɗaya daga sel kuma yana tabbatar da kwanciyar hankali na ƙwayar sinadarai., ba a sami wani gagarumin canji ba a karkashin firiji don 48 h.
Rubutun roba na inert ya cika da heparin, wanda zai iya cimma manufar saurin rabuwa da plasma, kuma ana iya adana samfurin na dogon lokaci.Za a iya amfani da hoses na rabuwa da aka kwatanta a sama don saurin binciken kwayoyin halitta.Separation gel heparin tubes sun dace da gwajin kwayoyin halitta a cikin gaggawa, sashin kulawa mai zurfi (ICU), da sauransu. abun da ke ciki na serum (plasma) na iya zama barga na dogon lokaci, wanda ya dace da sufuri.
2. Anticoagulant
1) Heparin tube (koren hula): Heparin wani kyakkyawan maganin rigakafi ne, wanda ba shi da tsangwama ga abubuwan da ke cikin jini, ba ya shafar adadin jajayen ƙwayoyin jini, kuma baya haifar da hemolysis.Ƙarar, erythrocyte sedimentation rate da general biochemical ƙuduri.
2) Blood routine tube (purple cap): EDTA ana cheated da calcium ions a cikin jini, ta yadda jini baya coagulate.Gabaɗaya, 1.0 ~ 2.0 MG na iya hana 1 ml na jini daga coagulating.Wannan maganin rigakafi baya shafar ƙididdigewa da girman ƙwayoyin farin jini, yana da ƙarancin tasiri akan tsarin halittar jajayen ƙwayoyin jini, kuma yana iya hana haɗuwar platelet, don haka ya dace da gwajin jini na gabaɗaya.Yawancin lokaci, ana amfani da hanyar spraying don yin reagent a ko'ina ya manne da bangon bututu, ta yadda samfurin jini zai iya zama da sauri da kuma cikakke gauraye bayan samfurin.
3) Blood coagulation tube (blue cap): yawan ruwa sodium citrate anticoagulant buffer ana saka shi cikin bututun tarin jini.Anticoagulant da ƙididdige adadin tarin jini ana ƙara su a cikin rabo na 1: 9 don nazarin kayan aikin coagulation (kamar PT, APTT).Ka'idar maganin ƙwanƙwasawa ita ce haɗawa da calcium don samar da sinadarin calcium chelate mai narkewa don kada jini ya tashe.Matsayin da aka ba da shawarar maganin jijiyoyi da ake buƙata don ƙididdigar hemagglutination shine 3.2% ko 3.8%, wanda yayi daidai da 0.109 ko 0.129 mol/L.Don gwajin coagulation na jini, idan rabon jini ya yi ƙasa sosai, za a tsawaita lokacin APTT, kuma sakamakon lokacin prothrombin (PT) shima zai canza sosai.Sabili da haka, ko rabon maganin jijiyoyi zuwa ƙimar tarin jini daidai ne ko a'a ya dogara da irin wannan samfurin.muhimmin ma'auni na inganci.
4) Bututun ESR (black cap): Tsarin hana zubar jini na bututun tarin jini iri daya ne da na bututun coagulation na jini, sai dai ana kara sinadarin sodium citrate anticoagulant da adadin tarin jinin da aka tantance a cikin wani rabo na 1:4 na ESR. jarrabawa.
5) Bututun glucose na jini (launin toka): Ana ƙara fluoride a cikin bututun tattara jini azaman mai hanawa.Saboda ƙarin mai hanawa da kulawa ta musamman na bangon ciki na bututun gwajin, ana kiyaye ainihin kaddarorin samfurin jini na dogon lokaci, kuma metabolism na sel jini yana da tushe.Ana amfani dashi sosai a cikin gwajin glucose na jini, haƙurin glucose, erythrocyte electrophoresis, hemoglobin anti-alkali, da haemolysis na glucose.
Lokacin aikawa: Maris-09-2022
