Hoʻokaʻawale a me ka wehewehe ʻana opaipu ohi koko
1. Biochemical
Hoʻokaʻawale ʻia nā paipu hōʻiliʻili koko biochemical i nā paipu hoʻohui ʻole (pale ʻulaʻula), nā paipu hoʻoikaika coagulation (pale ʻalani-ʻulaʻula), a me nā paipu hoʻokaʻawale (pale melemele).
ʻO ka paia o loko o ka paipu hōʻiliʻili koko kiʻekiʻe e hoʻohui ʻole ʻia i uhi ʻia me ka lāʻau lapaʻau i loko o ka paia a me ka mea mālama waha waha e pale aku ai i ka haki ʻana o ka cell i ka wā centrifugation a pili i nā hopena hōʻike, a maopopo ka paia o loko o ka paipu a me ka serum. a akaka, aohe koko e kau ana ma ka waha o ka paipu.
Ma waho aʻe o ka paia o loko o ka paipu coagulation i hoʻopaʻa ʻia me ka mea lapaʻau o ka paia o loko a me ka lāʻau lapaʻau nozzle, ua hoʻohana ʻia ke ʻano hoʻoheheʻe i loko o ka paipu e hoʻopili pono ai ka coagulation accelerator i ka pā paipu, kahi kūpono no ka wikiwiki. a hoʻohui piha i ke koko ma hope o ka laʻana, hiki ke hōʻemi nui i ka manawa coagulation.A ʻaʻohe ua o nā filament fibrin e pale aku ai i ka pinhole o nā mea hana i ka wā o ka laʻana.
Ke hoʻokaʻawale ʻia ka pahu hoʻokaʻawale, hoʻoneʻe ʻia ka gel separation i ke kikowaena o ka paipu, aia ma waena o ka serum a i ʻole ka plasma a me nā mea i hana ʻia i ke koko.Ma hope o ka pau ʻana o ka centrifugation, paʻa ia e hana i kahi pale, kahi e hoʻokaʻawale loa ai i ka serum a i ʻole ka plasma mai nā cell a hōʻoia i ka paʻa o ka haku mele serum., ʻaʻohe loli nui i ʻike ʻia ma lalo o ka refrigeration no 48 mau hola.
Hoʻopiha ʻia ka pahu hoʻokaʻawale inert me ka heparin, hiki ke hoʻokō i ke kumu o ka hoʻokaʻawale wikiwiki ʻana o ka plasma, a hiki ke mālama ʻia ka laʻana no ka manawa lōʻihi.Hiki ke hoʻohana ʻia nā paipu hoʻokaʻawale i hōʻike ʻia ma luna no ka hoʻāʻo biochemical wikiwiki.He kūpono ka separation gel heparin tubes no ka hoʻāʻo biochemical i ka ulia pōpilikia, acute intensive care unit (ICU), etc. Ke hoʻohālikelike ʻia me ka serum tube, ʻo ka pōmaikaʻi nui loa ʻo ia ka hiki ke hoʻokaʻawale koke ʻia ka serum (plasma), a ʻo ka lua o ka kemika. ʻO ka hoʻohui ʻana o ka serum (plasma) hiki ke kūpaʻa no ka manawa lōʻihi, kahi kūpono no ka lawe ʻana.
2. Anticoagulant
1) Heparin tube (poʻo ʻōmaʻomaʻo): He anticoagulant maikaʻi loa ʻo Heparin, ʻaʻole i hoʻopilikia iki i nā ʻāpana koko, ʻaʻole pili i ka nui o nā ʻulaʻula ʻulaʻula, a ʻaʻole hoʻi i ka hemolysis.Volume, erythrocyte sedimentation rate and general biochemical determination.
2) ʻO ka paipu maʻamau o ke koko (pale ʻulaʻula): Hoʻopili ʻia ʻo EDTA me nā ion calcium i loko o ke koko, i ʻole e coagulate ke koko.ʻO ka maʻamau, hiki i ka 1.0 ~ 2.0 mg ke pale i ka 1 ml o ke koko mai ka coagulating.ʻAʻole pili kēia anticoagulant i ka helu a me ka nui o nā keʻokeʻo keʻokeʻo keʻokeʻo, he liʻiliʻi ka hopena i ka morphology o nā ʻulaʻula ʻulaʻula, a hiki ke kāohi i ka hōʻuluʻulu ʻana o nā platelets, no laila kūpono ia no nā hoʻokolohua hematological maʻamau.ʻO ka maʻamau, hoʻohana ʻia ke ʻano o ka pīpī ʻana i mea e hoʻopili pono ai ka reagent i ka paia o ka paipu, i hiki ke hoʻopili koke ʻia ke koko ma hope o ka laʻana.
3) ʻO ka paipu coagulation koko (pouliuli): hoʻohui ʻia ka nui o ka wai sodium citrate anticoagulant buffer i ka paipu hōʻiliʻili koko.Hoʻohui ʻia ka Anticoagulant a me ka nui o ka hōʻiliʻili koko i ka ratio o 1:9 no ka nānā ʻana i nā mea hana coagulation (e like me PT, APTT).ʻO ke kumu o ka anticoagulation e hui pū me ka calcium e hana i ka chelate calcium soluble i ʻole e coagulate ke koko.ʻO ka manaʻo anticoagulant i koi ʻia no ka hemagglutination assays he 3.2% a i ʻole 3.8%, ʻo ia ka mea like me 0.109 a i ʻole 0.129 mol/L.No ka hoʻāʻo ʻana i ka coagulation koko, inā haʻahaʻa loa ka ratio o ke koko, e hoʻolōʻihi ʻia ka manawa APTT, a e hoʻololi nui ʻia nā hopena prothrombin time (PT).No laila, inā pololei a pololei ʻole ka ratio o ka anticoagulant i ka nui o ka hōʻiliʻili koko i kēia ʻano huahana.kūlana koʻikoʻi o ka maikaʻi.
4) ESR paipu (pale ʻeleʻele): ʻO ka ʻōnaehana anticoagulation o ka puʻu hōʻiliʻili koko e like me ka mea o ka paipu coagulation koko, koe wale nō ka sodium citrate anticoagulant a me ka nui o ka hōʻiliʻili koko i hoʻohui ʻia i ka ratio o 1:4 no ESR hoʻokolokolo.
5) Pahu koko koko (hina): Hoʻohui ʻia ka Fluoride i ka paipu hōʻiliʻili koko ma ke ʻano he mea pale.Ma muli o ka hoʻohui ʻana o ka inhibitor a me ka hoʻomaʻamaʻa kūikawā o ka paia o loko o ka paipu hoʻāʻo, mālama ʻia nā waiwai kumu o ka hāpana koko no ka manawa lōʻihi, a ʻo ka metabolism o nā pūnaewele koko ke kumu stagnant.Hoʻohana nui ʻia ia i ka nānā ʻana i ka glucose koko, ka hoʻomanawanui glucose, erythrocyte electrophoresis, anti-alkali hemoglobin, a me ka hemolysis glucose.
Ka manawa hoʻouna: Mar-09-2022
