Nhazi na nkọwa nketubes nchịkọta ọbara
1. Biochemical
A na-ekewa tubes na-anakọta ọbara biochemical gaa na tubes na-enweghị mgbakwunye (okpu uhie), tubes na-akwalite coagulation (okpu okpu na-acha ọbara ọbara), na tubes roba nkewa (okpu odo).
N'ime mgbidi nke elu-edu mmako-free ọbara collection tube bụ evenly ntekwasa na n'ime mgbidi ọgwụgwọ gị n'ụlọnga na tube ọnụ ọgwụgwọ gị n'ụlọnga iji zere mmebi cell n'oge centrifugation na-emetụta ule pụta, na n'ime mgbidi nke tube na serum doro anya. na nghọta, ma ọ dịghị ọbara nghọta na tube ọnụ.
Na mgbakwunye na mgbidi dị n'ime nke coagulation tube na-ejikọta ya na onye na-ahụ maka ọgwụgwọ mgbidi dị n'ime na onye na-ahụ maka ọgwụgwọ nozzles, a na-anabata usoro ịgbasa n'ime tube ahụ iji mee ka onye na-eme ihe na-eme ka ọkpụkpụ na-ejikọta ya na mgbidi tube, nke dị mma maka ngwa ngwa. na ịgwakọta ihe nlele ọbara n'ụzọ zuru oke ka emechara, nke nwere ike belata oge coagulation nke ukwuu.Na enweghị mmiri ozuzo nke fibrin filaments iji zere igbochi pinhole nke akụrụngwa n'oge nlele.
Mgbe nkewa roba tube bụ centrifuged, na nkewa gel na-akpali n'etiti tube, nke dị n'etiti serum ma ọ bụ plasma na ọbara mejupụtara components.Mgbe centrifugation mechara, ọ na-eme ka ọ bụrụ ihe mgbochi, nke na-ekewapụ serum ma ọ bụ plasma kpamkpam na sel ma na-eme ka nkwụsi ike nke ihe mejupụtara kemịkalụ serum., ọ dịghị mgbanwe dị ịrịba ama n'okpuru refrigeration maka 48 h.
The inert nkewa roba tube juputara na heparin, nke nwere ike mezuo nzube nke ngwa ngwa nkewa nke plasma, na sample nwere ike ịchekwa ruo ogologo oge.Enwere ike iji hoses nkewa nke akọwara n'elu maka nyocha biochemical ngwa ngwa.Gel heparin tubes nkewa dị mma maka nyocha biochemical na mberede, nnukwu nlekọta nlekọta ahụike (ICU), wdg. E jiri ya tụnyere tube serum, nnukwu uru bụ na a na-ekewa ọbara (plasma) ngwa ngwa, nke abụọ bụ na kemịkal ahụ. Ngwakọta nke serum (plasma) nwere ike kwụsie ike ruo ogologo oge, nke dị mma maka njem.
2. Anticoagulant
1) Ọkpụkpụ Heparin (okpu akwụkwọ ndụ akwụkwọ ndụ): Heparin bụ ezigbo anticoagulant, nke na-enwe obere nnyonye anya na ihe mejupụtara ọbara, anaghị emetụta olu nke mkpụrụ ndụ ọbara uhie, ọ naghị ebute hemolysis.Olu, erythrocyte sedimentation ọnụego na n'ozuzu biochemical mkpebi siri ike.
2) Ọbara na-emekarị tube (okpu na-acha odo odo): EDTA na-eji calcium ion n'ime ọbara chechie ya, ka ọbara ghara ịgbakọta.N'ozuzu, 1.0 ~ 2.0 mg nwere ike igbochi 1 ml nke ọbara coagulating.Ọgwụ mgbochi a anaghị emetụta ọnụ ọgụgụ na nha nke mkpụrụ ndụ ọbara ọcha, ọ na-enwe mmetụta dị nta na morphology nke mkpụrụ ndụ ọbara uhie, ma nwee ike igbochi mkpokọta platelet, yabụ ọ dabara maka nyocha ọbara ọbara n'ozuzu.Na-emekarị, usoro nke spraying na-nakweere na-eme ka reagent evenly rube isi na tube mgbidi, nke mere na ọbara pụrụ ịbụ ngwa ngwa na n'ụzọ zuru ezu gwakọtara mgbe sample.
3) Ọbara coagulation tube (okpu na-acha anụnụ anụnụ): ọnụọgụ mmiri sodium citrate anticoagulant buffer na-agbakwunye na tube mkpokọta ọbara.A na-agbakwunye ọgwụ mgbochi na ọkwa mkpokọta ọbara na nha 1: 9 maka nyocha nke ihe ndị na-eme ka coagulation (dị ka PT, APTT).Ụkpụrụ nke anticoagulation bụ ijikọta ya na calcium iji mepụta calcium chelate soluble ka ọbara ghara ịgbakọta.Ntinye uche nke anticoagulant akwadoro achọrọ maka nyocha hemagglutination bụ 3.2% ma ọ bụ 3.8%, nke dabara na 0.109 ma ọ bụ 0.129 mol/L.Maka ule coagulation ọbara, ọ bụrụ na oke ọbara dị ntakịrị, oge APTT ga-adị ogologo, na oge prothrombin (PT) ga-agbanwekwa nke ukwuu.Ya mere, ma oke nke anticoagulant na oke nchịkọta ọbara akwadoro ziri ezi ma ọ bụ na ọ dabere na ụdị ngwaahịa a.ụkpụrụ dị mkpa nke ịdị mma.
4) ESR tube (okpu ojii): Usoro mgbochi mgbochi nke tube mkpokọta ọbara bụ otu ihe ahụ nke tube coagulation ọbara, ma e wezụga na sodium citrate anticoagulant na ọkwa mkpokọta ọbara na-agbakwunye na nha 1: 4 maka ESR. nyocha.
5) Ọbara glucose tube (isi awọ): A na-agbakwunye fluoride na tube nchịkọta ọbara dị ka ihe mgbochi.N'ihi mgbakwunye nke inhibitor na ọgwụgwọ pụrụ iche nke mgbidi dị n'ime nke tube ule ahụ, a na-edobe ihe mbụ nke ihe nlele ọbara ruo ogologo oge, na metabolism nke mkpụrụ ndụ ọbara na-adaba adaba.A na-eji ya n'ọtụtụ ebe na nyocha nke glucose ọbara, nnabata glucose, erythrocyte electrophoresis, hemoglobin anti-alkali, na hemolysis glucose.
Oge nzipu: Mar-09-2022
