Ukuhlelwa kunye nenkcazo yeiityhubhu zokuqokelela igazi
1. Amachiza ezinto eziphilayo
Imibhobho yokuqokelela igazi ye-biochemical yahlulwe ibe yimibhobho edityanisiweyo (i-cap ebomvu), iityhubhu ezikhuthaza ukuhlangana (i-orenji-red cap), kunye netyhubhu yerabha yokwahlula (ikepusi etyheli).
Udonga lwangaphakathi lwetyhubhu yokuqokelela igazi ekumgangatho ophezulu edityanisiweyo idityaniswe ngokulinganayo kunye ne-arhente yonyango lwangaphakathi kunye ne-ejenti yonyango yomlomo ityhubhu ukuphepha ukuphuka kweeseli ngexesha le-centrifugation kunye nokuchaphazela iziphumo zovavanyo, kwaye udonga lwangaphakathi lwetyhubhu kunye neserum zicacile. kwaye ekuhleni, kwaye akukho gazi lijinga emlonyeni ityhubhu.
Ukongeza kudonga lwangaphakathi lwetyhubhu ye-coagulation edityaniswe ngokufanayo kunye nearhente yonyango yangaphakathi yodonga kunye nearhente yonyango lombhobho, indlela yokutshiza yamkelwa kumbhobho ukwenza i-accelerator ye-coagulation idityaniswe ngokulinganayo eludongeni lwetyhubhu, olulungele ngokukhawuleza. kunye nokuxuba ngokupheleleyo isampuli yegazi emva kwesampulu, enokunciphisa kakhulu ixesha lokudibanisa.Kwaye akukho mvula ye-fibrin filaments ukuphepha ukuvala i-pinhole yesixhobo ngexesha lokuthatha isampuli.
Xa ityhubhu yerabha yokwahlula i-centrifuged, ijeli yokwahlula ifuduselwa kumbindi wombhobho, ophakathi kwe-serum okanye iplasma kunye namacandelo egazi.Emva kokuba i-centrifugation igqityiwe, iqinisa ukwenza umqobo, eyahlula ngokupheleleyo i-serum okanye i-plasma kwiiseli kwaye iqinisekisa ukuzinza kwe-serum chemical composition., akukho tshintsho olubalulekileyo olubonwe phantsi kwefriji ye-48 h.
I-tube ye-rubber yokuhlukana kwe-inert igcwele i-heparin, enokuthi ifezekise injongo yokuhlukana ngokukhawuleza kwe-plasma, kwaye isampuli inokugcinwa ixesha elide.Imibhobho yokwahlula echazwe ngasentla ingasetyenziselwa ukuvavanya ngokukhawuleza kwe-biochemical.Ukwahlula iityhubhu ze-gel heparin zilungele uvavanyo lwe-biochemical kwimeko yongxamiseko, iyunithi yokhathalelo olunzulu (ICU), njl njl. ukubunjwa kwe-serum (i-plasma) inokuzinza ixesha elide, elilungele ukuthutha.
2. I-Anticoagulant
I-1) I-Heparin ityhubhu (i-cap eluhlaza): I-Heparin yi-anticoagulant egqwesileyo, enokuphazamiseka okuncinci kumalungu egazi, ayichaphazeli umthamo weeseli ezibomvu zegazi, kwaye ayibangeli i-hemolysis.Umthamo, izinga le-erythrocyte sedimentation kunye nokuzimisela ngokubanzi kwe-biochemical.
I-2) I-tube ye-blood routine (i-cap emfusa): I-EDTA i-chelated kunye ne-calcium ions egazini, ukwenzela ukuba igazi lingadibanisi.Ngokuqhelekileyo, i-1.0 ~ 2.0 mg inokuthintela i-1 ml yegazi ekudibaniseni.Le anticoagulant ayichaphazeli ukubala kunye nobukhulu beeseli ezimhlophe zegazi, inefuthe elincinci kwi-morphology yeeseli ezibomvu zegazi, kwaye inokuthintela ukudityaniswa kweeplatelet, ngoko ifanelekile kwiimvavanyo ze-hematological jikelele.Ngokuqhelekileyo, indlela yokutshiza iyamkelwa ukwenza i-reagent ibambelele ngokulinganayo eludongeni lombhobho, ukwenzela ukuba isampuli yegazi inokukhawuleza kwaye ixutywe ngokupheleleyo emva kwesampuli.
I-3) I-tube ye-blood coagulation (i-cap eluhlaza okwesibhakabhaka): ubungakanani be-sodium citrate anticoagulant buffer yongezwa kwi-tube yokuqokelela igazi.I-Anticoagulant kunye nexabiso lokuqokelela igazi elilinganisiweyo longezwa kwi-1: 9 yokuhlolwa kwezinto ze-coagulation mechanism (njenge-PT, i-APTT).Umgaqo we-anticoagulation kukudibanisa ne-calcium ukwenza i-calcium chelate e-soluble ukwenzela ukuba igazi lingadibanisi.I-concentration ye-anticoagulant ekhuthazwayo efunekayo kwi-hemagglutination assays yi-3.2% okanye i-3.8%, elingana ne-0.109 okanye i-0.129 mol / L.Kuvavanyo lwe-coagulation yegazi, ukuba umlinganiselo wegazi uphantsi kakhulu, ixesha le-APTT liya kwandiswa, kwaye iziphumo zeprothrombin (PT) nazo ziya kutshintshwa kakhulu.Ngoko ke, ukuba umlinganiselo we-anticoagulant ukuya kumthamo wokuqokelela igazi uchanekile okanye awuxhomekeke kulolu hlobo lwemveliso.umgangatho obalulekileyo womgangatho.
I-4) ityhubhu ye-ESR (i-black cap): Inkqubo ye-anticoagulation ye-tube yokuqokelela igazi iyafana neyombhobho we-coagulation yegazi, ngaphandle kokuba i-anticoagulant ye-sodium citrate kunye nomthamo wokuqokelela igazi wongezwa kwi-1: 4 ye-ESR. uviwo.
I-5) I-tube ye-glucose yegazi (igrey): I-Fluoride yongezwa kwi-tube yokuqokelela igazi njenge-inhibitor.Ngenxa yokongezwa kwe-inhibitor kunye nonyango olukhethekileyo lodonga lwangaphakathi lwetyhubhu yokuvavanya, iimpawu zokuqala zesampulu yegazi zigcinwa ixesha elide, kwaye i-metabolism yeeseli zegazi ngokusisiseko imile.Isetyenziswa ngokubanzi kuvavanyo lweswekile yegazi, ukunyamezela iswekile, i-erythrocyte electrophoresis, i-anti-alkali hemoglobin, kunye ne-glucose hemolysis.
Ixesha lokuposa: Mar-09-2022
