Lub tshuab nqus tsev ntim cov hlab ntsha yog lub tshuab nqus tsev tsis zoo siv lub tshuab nqus tsev vacuum uas tuaj yeem paub txog kev sau cov ntshav ntau.Nws yuav tsum tau siv ua ke nrog koob sau ntshav venous.
Lub hauv paus ntsiab lus ntawm lub tshuab nqus tsev sau ntshav
Lub hauv paus ntsiab lus ntawm lub tshuab nqus tsev sau cov ntshav yog kos cov ntshav sau raj nrog lub taub hau lub taub hau rau hauv lub tshuab nqus tsev sib txawv ua ntej, siv nws lub siab tsis zoo los txiav txim siab ntau npaum li cas sau cov ntshav venous, thiab ntxig ib kawg ntawm rab koob sau ntshav rau hauv tib neeg cov leeg thiab lwm qhov kawg rau hauv cov roj hmab ntsaws ntawm lub tshuab nqus ntshav sau raj.Tib neeg cov ntshav venous yog nyob rau hauv lub tshuab nqus tsev vacuum.Nyob rau hauv qhov kev txiav txim ntawm qhov tsis zoo siab, nws yog pumped mus rau hauv lub thawv ntim ntshav los ntawm cov koob sau ntshav.Nyob rau hauv ib lub venipuncture, ntau lub raj sau tuaj yeem pom tau yam tsis muaj qhov xau.Qhov ntim ntawm lub lumen txuas cov koob sau cov ntshav yog qhov tsawg heev, yog li qhov cuam tshuam ntawm cov ntshav ntim ntim tuaj yeem tsis quav ntsej, tab sis qhov tshwm sim ntawm qhov sib txawv yog qhov me me.Piv txwv li, qhov ntim ntawm lub lumen yuav noj ib feem ntawm lub tshuab nqus tsev ntawm cov hlab ntsha sau cov ntshav, yog li txo qhov ntim ntim.
Classification ntawm lub tshuab nqus tsev sau cov hlab ntsha
Raws li pom nyob rau hauv daim duab 1, muaj 9 hom ntawm lub tshuab nqus tsev vacuum sau cov hlab ntsha, uas yuav txawv raws li cov xim ntawm lub hau.
Daim duab 1 hom nqus cov hlab ntshav
1. hom ntshav ntshav liab cap
Cov hlab ntsha sau cov ntshav tsis muaj tshuaj ntxiv, tsis muaj cov tshuaj tiv thaiv coagulant thiab procoagulant, tsuas yog lub tshuab nqus tsev.Nws yog siv rau niaj hnub serum biochemistry, ntshav txhab nyiaj thiab serology hais txog kev kuaj, ntau yam biochemical thiab immunological kuaj, xws li syphilis, kab mob siab B kom muaj nuj nqis, thiab lwm yam. nws tsis tas yuav co tom qab ntshav kos.Hom kev npaj cov qauv yog cov ntshav.Tom qab cov ntshav kos duab, nws muab tso rau hauv 37 ℃ dej da dej rau ntau tshaj 30min, centrifuged, thiab cov ntshav siab yog siv rau standby.
2. txiv kab ntxwv cap ntawm ceev cov hlab ntsha
Muaj cov coagulants nyob rau hauv cov hlab ntsha sau cov hlab ntsha kom ceev cov txheej txheem coagulation.Cov hlab ntsha sai sai tuaj yeem coagulate cov ntshav sau hauv 5 feeb.Nws yog qhov tsim nyog rau kev kuaj ntshav thaum muaj xwm txheej ceev.Nws yog cov feem ntau siv coagulation txhawb kev kuaj tube rau txhua hnub biochemistry, kev tiv thaiv kab mob, ntshav, cov tshuaj hormones, thiab lwm yam. tom qab kev nqus ntshav, nws tuaj yeem thim rov qab thiab sib xyaw rau 5-8 zaug.Thaum chav tsev kub qis, nws tuaj yeem muab tso rau hauv 37 ℃ dej da dej rau 10-20min, thiab cov ntshav siab tuaj yeem centrifuged rau standby.
3. golden hau npog ntawm inert sib cais gel accelerating raj
Inert gel thiab coagulant tau ntxiv rau hauv cov hlab ntshav.Cov qauv tseem nyob ruaj khov hauv 48 teev tom qab centrifugation.Lub coagulant tuaj yeem ua kom lub coagulation mechanism sai sai thiab ua kom cov txheej txheem coagulation.Cov qauv qauv yog cov ntshav, uas yog tsim rau thaum muaj xwm ceev serum biochemical thiab pharmacokinetic kuaj.Tom qab sau, sib tov nws upside down rau 5-8 zaug, sawv ntsug rau 20-30min, thiab centrifuge tus supernatant siv.
4. dub cap ntawm sodium citrate ESR xeem raj
Qhov xav tau ntawm sodium citrate rau kev kuaj ESR yog 3.2% (sib npaug rau 0.109mol / l), thiab qhov piv ntawm cov tshuaj tiv thaiv rau cov ntshav yog 1: 4.Nws muaj 0.4 ml ntawm 3.8% sodium citrate.Kos ntshav rau 2.0 ml.Qhov no yog ib lub raj tshwj xeeb rau erythrocyte sedimentation tus nqi.Hom qauv yog plasma.Nws yog haum rau erythrocyte sedimentation tus nqi.Tom qab nqus cov ntshav, nws yog tam sim ntawd thim rov qab thiab sib tov rau 5-8 zaug.Co zoo ua ntej siv.Qhov sib txawv ntawm nws thiab cov raj kuaj rau coagulation factor test yog qhov concentration ntawm anticoagulant sib txawv ntawm cov ntshav, uas tsis tuaj yeem tsis meej pem.
5. sodium citrate coagulation kuaj raj lub teeb xiav cap
Sodium citrate plays lub luag haujlwm tiv thaiv coagulant feem ntau los ntawm chelating nrog calcium ions hauv cov ntshav kuaj.Qhov concentration ntawm anticoagulant pom zoo los ntawm National Committee rau kev soj ntsuam kuaj tus qauv yog 3.2% lossis 3.8% (sib npaug rau 0.109mol / l lossis 0.129mol / l), thiab qhov piv ntawm anticoagulant rau ntshav yog 1: 9.Cov hlab ntsha nqus ntshav muaj txog 0.2ml ntawm 3.2% sodium citrate anticoagulant.Cov ntshav tau sau rau 2.0 ml.Cov qauv npaj hom yog tag nrho cov ntshav lossis ntshav.Tom qab sau, nws yog tam sim ntawd thim rov qab thiab sib tov rau 5-8 zaug.Tom qab centrifugation, lub plasma sab saud raug coj mus rau sawv ntsug.Nws yog tsim rau coagulation test, Pt, APTT thiab coagulation factor test.
6. heparin anticoagulation tube ntsuab cap
Heparin tau ntxiv rau hauv cov hlab ntshav.Heparin muaj cov nyhuv ntawm antithrombin ncaj qha, uas tuaj yeem ua kom ntev lub sijhawm coagulation ntawm cov qauv.Nws yog siv rau thaum muaj xwm txheej ceev thiab feem ntau cov kev sim biochemical, xws li lub siab ua haujlwm, lub raum ua haujlwm, ntshav lipid, ntshav qabzib, thiab lwm yam. Nws muaj feem xyuam rau kev kuaj ntshav liab fragility, kuaj ntshav roj, kuaj hematocrit, ESR thiab kev txiav txim siab dav dav biochemical, tsis yog. haum rau kev kuaj hemagglutination.Heparin ntau dhau tuaj yeem ua rau leukocyte aggregation thiab tsis tuaj yeem siv rau kev suav leukocyte.Nws tsis haum rau kev faib cov leukocyte vim tias nws tuaj yeem ua rau cov keeb kwm ntawm cov ntshav hlais dyed lub teeb xiav.Nws tuaj yeem siv rau hemorheology.Hom qauv yog plasma.Tam sim ntawd tom qab sau cov ntshav, thim rov qab thiab sib tov rau 5-8 zaug.Siv lub plasma sab saud rau kev tos txais.
7. lub teeb ntsuab lub taub hau npog ntawm plasma cais raj
Ntxiv heparin lithium anticoagulant rau hauv inert kev sib cais hose tuaj yeem ua tiav lub hom phiaj ntawm kev sib cais plasma sai.Nws yog qhov kev xaiv zoo tshaj plaws rau kev kuaj xyuas electrolyte.Nws kuj tseem tuaj yeem siv rau kev kuaj ntshav plasma biochemical niaj hnub thiab kuaj ntshav plasma biochemical thaum muaj xwm txheej xws li ICU.Nws yog siv thaum muaj xwm txheej ceev thiab feem ntau cov kev sim biochemical, xws li lub siab ua haujlwm, lub raum ua haujlwm, ntshav lipid, ntshav qabzib, thiab lwm yam. Cov qauv ntshav tuaj yeem muab tso ncaj qha rau ntawm lub tshuab thiab khaws cia ruaj khov rau 48 teev hauv qhov txias txias.Nws tuaj yeem siv rau hemorheology.Hom qauv yog plasma.Tam sim ntawd tom qab sau cov ntshav, thim rov qab thiab sib tov rau 5-8 zaug.Siv lub plasma sab saud rau kev tos txais.
8. potassium oxalate / sodium fluoride grey cap
Sodium fluoride yog ib qho tsis muaj zog tiv thaiv coagulant.Nws feem ntau yog siv ua ke nrog potassium oxalate lossis sodium ethyliodate.Qhov kev faib ua feem yog 1 feem ntawm sodium fluoride thiab 3 qhov chaw ntawm potassium oxalate.4mg ntawm qhov sib xyaw no tuaj yeem tiv thaiv 1ml ntawm cov ntshav los ntawm coagulation thiab inhibit cov piam thaj hauv 23 hnub.Nws tsis tuaj yeem siv rau kev txiav txim siab urea los ntawm txoj kev Urease, lossis kev txiav txim siab alkaline phosphatase thiab amylase.Nws raug pom zoo rau kev kuaj ntshav qabzib.Nws muaj sodium fluoride, potassium oxalate lossis EDTA Na tshuaj tsuag, uas tuaj yeem cuam tshuam cov kev ua haujlwm enolase hauv qabzib metabolism.Tom qab cov ntshav kos, nws yog thim rov qab thiab sib tov rau 5-8 zaug.Tom qab centrifugation, lub supernatant thiab plasma raug coj mus rau standby.Nws yog ib lub raj tshwj xeeb rau kev txiav txim siab sai ntawm cov ntshav qabzib.
9. EDTA anticoagulation yeeb nkab ntshav cap
Ethylenediaminetetraacetic acid (EDTA, molecular hnyav 292) thiab nws cov ntsev yog ib hom amino polycarboxylic acid, uas yog tsim rau kev kuaj hematology.Nws yog lub raj uas nyiam tshaj plaws rau cov ntshav niaj hnub, glycosylated hemoglobin thiab ntshav kuaj ntshav.Nws tsis muaj feem xyuam rau kev kuaj coagulation thiab platelet kev ua haujlwm, lossis kev txiav txim siab ntawm calcium ion, potassium ion, sodium ion, hlau ion, alkaline phosphatase, creatine kinase thiab leucine aminopeptidase.Nws yog haum rau kev kuaj PCR.Txau 100ml ntawm 2.7% edta-k2 tov rau ntawm phab ntsa sab hauv ntawm lub tshuab nqus tsev, tshuab kom qhuav ntawm 45 ℃, coj cov ntshav mus rau 2mi, tam sim ntawd thim rov qab thiab sib tov rau 5-8 zaug tom qab kev nqus ntshav, thiab tom qab ntawd sib tov rau kev siv.Hom qauv yog tag nrho cov ntshav, uas yuav tsum tau tov thaum siv.
Post lub sij hawm: Jun-29-2022
