Mgbochi ọbara na-anakọta arịa bụ ọkpọkọ ikuku ikuku na-adịghị mma nke nwere ike nweta mkpokọta ọbara.Ekwesịrị iji ya na agịga mkpokọta ọbara venous.
Ụkpụrụ nke nchịkọta ọbara agụụ
Ụkpụrụ nke nchịkọta ọbara agụụ bụ ịdọrọ tube mkpokọta ọbara nwere okpu isi n'ime ogo oghere dị iche iche tupu oge eruo, jiri nrụgide na-adịghị mma na-anakọta ihe nlele ọbara na-akpaghị aka na quantitatively, ma tinye otu njedebe nke agịga nchịkọta ọbara n'ime akwara mmadụ. nsọtụ nke ọzọ banye na plọg rọba nke oghere mkpokọta ọbara.Ọbara venous mmadụ dị n'ime oghere ọbara na-anakọta agụụ.N'okpuru omume nke nrụgide na-adịghị mma, a na-agbanye ya n'ime ihe nlele ọbara site na agịga nchịkọta ọbara.N'okpuru otu venipuncture, a ga-enweta nchịkọta ọtụtụ tube na-enweghị nkwụsị.Olu nke lumen na-ejikọta agịga nchịkọta ọbara dị ntakịrị, ya mere enwere ike ileghara mmetụta dị na mkpokọta mkpokọta ọbara anya, mana ohere nke countercurrent dị ntakịrị.Dịka ọmụmaatụ, olu nke lumen ga-eri akụkụ nke oghere nke arịa nchịkọta ọbara, si otú a na-ebelata mkpokọta mkpokọta.
Nhazi nke arịa mkpokọta ọbara agụụ
Dị ka e gosiri na foto 1, e nwere ụdị 9 nke oghere ọbara na-anakọta arịa, nke nwere ike ịmata ọdịiche dị ka agba nke mkpuchi ahụ.
Ụdị 1 ụdị arịa mkpokọta ọbara agụụ
1. nkịtị serum tube uhie okpu
Ụgbọ mmiri na-anakọta ọbara enweghị ihe mgbakwunye, ọ nweghị ihe mgbochi mgbochi na ihe mgbochi, naanị oghere.A na-eji ya maka biochemistry serum na-aga n'ihu, ụlọ akụ ọbara na nyocha ndị metụtara serology, ule dị iche iche biochemical na immunological, dị ka syphilis, ịba ọcha n'anya B, wdg. Ọ dịghị mkpa ịma jijiji mgbe eserese ọbara.Ụdị nkwadebe ihe atụ bụ serum.Mgbe eserese ọbara gasịrị, a na-etinye ya n'ime mmiri ịsa ahụ 37 ℃ maka ihe karịrị 30min, centrifuged, a na-eji serum elu maka njikere.
2. oroma okpu nke ngwa ngwa serum tube
Enwere coagulant na arịa mkpokọta ọbara iji mee ka usoro coagulation dịkwuo ngwa.Ọkpụkpụ serum ngwa ngwa nwere ike imechi ọbara anakọtara n'ime nkeji ise.Ọ dabara maka usoro nyocha serum mberede.Ọ bụ ihe a na-ejikarị eme ihe na-akwado coagulation tube maka biochemistry kwa ụbọchị, mgbochi, serum, hormones, wdg mgbe eserese ọbara gasịrị, enwere ike ịtụgharị ya ma gwakọta ya ugboro 5-8.Mgbe ọnụ ụlọ ahụ dị ntakịrị, enwere ike itinye ya n'ime mmiri ịsa ahụ 37 ℃ maka 10-20min, na serum nke elu nwere ike ịbụ centrifuged maka njikere.
3. ọla edo isi mkpuchi nke inert nkewa gel accelerating tube
A na-agbakwunye gel inert na coagulant na arịa nchịkọta ọbara.Ihe nlele ahụ kwụsiri ike n'ime awa 48 ka centrifugation gasịrị.Coagulant nwere ike ime ngwa ngwa usoro coagulation ma mee ka usoro coagulation dịkwuo ngwa.Ụdị ihe atụ bụ serum, nke kwesịrị ekwesị maka ihe mberede serum biochemical na pharmacokinetic test.Mgbe ịchịkọtachara, gwakọta ya n'akụkụ ihu ugboro 5-8, kwụ ọtọ maka nkeji 20-30, ma centrifute supernatant maka ojiji.
4. okpu ojii nke sodium citrate ESR test tube
Ntụnye sodium citrate achọrọ maka ule ESR bụ 3.2% (nke dabara na 0.109mol / l), yana oke anticoagulant na ọbara bụ 1: 4.Ọ nwere 0.4ml nke 3.8% sodium citrate.Were ọbara na 2.0ml.Nke a bụ tube ule pụrụ iche maka ọnụ ọgụgụ sedimentation erythrocyte.Ụdị ihe atụ bụ plasma.Ọ dabara maka ọnụ ọgụgụ sedimentation erythrocyte.Mgbe ọ gbasịrị ọbara, a na-atụgharị ya ozugbo wee gwakọta ya ugboro 5-8.Ma jijiji nke ọma tupu eji ya.Ọdịiche dị n'etiti ya na tube ule maka nyocha ihe na-akpata coagulation bụ na ntinye nke anticoagulant dị iche na oke ọbara, nke enweghị ike ịgbagwoju anya.
5. sodium citrate coagulation test tube ìhè-acha anụnụ anụnụ okpu
Sodium citrate na-arụ ọrụ mgbochi mgbakasị ahụ tumadi site na iji ion calcium chelating na nlele ọbara.Nleba anya nke anticoagulant nke Kọmitii Mba kwadoro maka nhazi ụlọ nyocha ụlọ ọgwụ bụ 3.2% ma ọ bụ 3.8% (nke dabara na 0.109mol / l ma ọ bụ 0.129mol / l), na nha nke anticoagulant na ọbara bụ 1: 9.Ụgbọ mmiri na-anakọta agụụ nwere ihe dịka 0.2ml nke 3.2% sodium citrate anticoagulant.A na-anakọta ọbara na 2.0ml.Ụdị nkwadebe sample bụ ọbara dum ma ọ bụ plasma.Mgbe nchịkọta gasịrị, a na-atụgharị ya ozugbo wee gwakọta ya ugboro 5-8.Mgbe centrifugation gasịrị, a na-ewere plasma dị elu maka njikere.Ọ dabara maka ule coagulation, Pt, APTT na ule coagulation factor.
6. heparin anticoagulation tube okpu akwụkwọ ndụ akwụkwọ ndụ
A na-agbakwunye Heparin na arịa nchịkọta ọbara.Heparin nwere mmetụta antithrombin ozugbo, nke nwere ike ịgbatị oge coagulation nke ihe nlele.A na-eji ya na mberede na ọtụtụ nyocha biochemical, dị ka ọrụ imeju, ọrụ akụrụ, ọbara lipid, glucose ọbara, wdg. dị mma maka ule hemaglutination.Heparin dị ukwuu nwere ike ime ka nchịkọta leukocyte na enweghị ike iji ya mee ihe maka ịgụta leukocytes.Ọ dịghị mma maka nhazi ọkwa leukocyte n'ihi na ọ nwere ike ime ka ndabere nke iberi ọbara siji acha anụnụ anụnụ.Enwere ike iji ya maka hemorheology.Ụdị ihe atụ bụ plasma.Ozugbo nchịkọta ọbara gasịrị, tụgharịa ma gwakọta ya ugboro 5-8.Were plasma dị elu maka njikere.
7. ìhè akwụkwọ ndụ akwụkwọ ndụ isi mkpuchi nke tube nkewa nkewa
Ịgbakwunye heparin lithium anticoagulant n'ime eriri nkewa inert nwere ike mezuo ebumnuche nkewapụ plasma ngwa ngwa.Ọ bụ nhọrọ kachasị mma maka nchọpụta electrolyte.Enwere ike iji ya maka nchọpụta biochemical plasma oge niile yana nchọpụta biochemical nke plasma mberede dị ka ICU.A na-eji ya na mberede na ọtụtụ nnwale biochemical, dị ka ọrụ imeju, ọrụ akụrụ, lipid ọbara, glucose ọbara, wdg. Enwere ike itinye ihe nlele Plasma ozugbo na igwe ma debe ya maka awa 48 n'okpuru nchekwa oyi.Enwere ike iji ya maka hemorheology.Ụdị ihe atụ bụ plasma.Ozugbo nchịkọta ọbara gasịrị, tụgharịa ma gwakọta ya ugboro 5-8.Were plasma dị elu maka njikere.
8. potassium oxalate / sodium fluoride isi awọ okpu
Sodium fluoride bụ ọgwụ mgbochi na-adịghị ike.A na-ejikarị ya na potassium oxalate ma ọ bụ sodium ethyliodate.Nkezi bụ 1 akụkụ nke sodium fluoride na 3 akụkụ nke potassium oxalate.4mg nke ngwakọta a nwere ike igbochi 1ml ọbara site na coagulation ma gbochie ire ere shuga n'ime ụbọchị 23.Enweghị ike iji ya maka mkpebi urea site na usoro Urease, ma ọ bụ maka mkpebi alkaline phosphatase na amylase.A na-atụ aro ya maka ịchọpụta glucose ọbara.Ọ nwere sodium fluoride, potassium oxalate ma ọ bụ EDTA Na ịgba, nke nwere ike igbochi ọrụ enolase na glucose metabolism.Mgbe eserese ọbara gasịrị, a na-atụgharị ya ma gwakọta ya ugboro 5-8.Mgbe centrifugation gasịrị, a na-ewere supernatant na plasma maka njikere.Ọ bụ tube pụrụ iche maka ikpebi ọkwa glucose ọbara ngwa ngwa.
9. EDTA anticoagulation ọkpọkọ odo odo okpu
Ethylenediaminetetraacetic acid (EDTA, molecular weight 292) na nnu ya bụ ụdị amino polycarboxylic acid, nke dabara maka ule ọbara ọbara n'ozuzu.Ọ bụ tube ule kacha amasị maka usoro ọbara, glycosylated haemoglobin na ule otu ọbara.Ọ dịghị adabara maka ule coagulation na ule platelet, ma ọ bụ na mkpebi nke calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase na leucine aminopeptidase.Ọ dabara maka ule PCR.Fesa 100ml nke 2.7% edta-k2 na mgbidi dị n'ime nke oghere oghere ahụ, kpoo na 45 ℃, were ọbara gaa na 2mi, tụgharịa ozugbo ma gwakọta ya ugboro 5-8 mgbe eserese ọbara gasịrị, wee gwakọta ya maka ojiji.Ụdị nlele ahụ bụ ọbara zuru oke, nke kwesịrị ịgwakọta mgbe ejiri ya.
Oge nzipu: Jun-29-2022
