Ko te ipu kohi toto korehau he ngongo karaihe korehau pehanga kino e taea te mohio ki te kohinga toto ine.Me whakamahi tahi me te ngira kohinga toto venous.
Te kaupapa o te kohinga toto korehau
Ko te kaupapa o te kohinga toto korehau ko te tuhi i te ngongo kohinga toto me te potae mahunga ki nga nekehanga rereke rereke i mua, te whakamahi i tana pehanga kino ki te kohikohi aunoa me te ine i nga tauira toto toto, me te whakauru i tetahi pito o te ngira kohinga toto ki roto i te uaua o te tangata. te tahi pito ki roto i te mono rapa o te ngongo kohinga toto korehau.Ko te toto uaua tangata kei roto i te ipu kohi toto korehau.I raro i te kaha o te pehanga kino, ka panaia ki roto i te ipu tauira toto ma te ngira kohinga toto.I raro i tetahi venipuncture, ko te kohinga ngongo maha ka taea te mohio me te kore e pakaru.He iti rawa te ruri o te lumen e hono ana i te ngira kohinga toto, na reira ka kore e arohia te paanga ki te kohinga toto, engari he iti noa te tupono o te taupatupatu.Hei tauira, ko te nui o te lumen ka pau tetahi wahi o te korehau o te ipu kohinga toto, na reira ka whakaitihia te kohinga kohinga.
Te whakarōpūtanga o nga oko kohi toto korehau
Ka rite ki te Whakaaturanga 1, e 9 nga momo oko kohinga toto korehau, ka taea te wehewehe i runga i te tae o te uhi.
Whakaaturia 1 nga momo oko kohi toto korehau
1. ngongo serum noa potae whero
Karekau he taapiri i roto i te ipu kohinga toto, karekau he waahanga anticoagulant me te procoagulant, ko te korehau anake.Ka whakamahia mo nga whakamatautau koiora serum, peeke toto me nga whakamatautau e pa ana ki te serology, nga momo whakamatautau matū koiora me te immunological, penei i te syphilis, te ine mate hepatitis B, me etahi atu kaore e tika kia wiri i muri i te tohanga toto.Ko te momo whakarite tauira he serum.I muri i te tohanga toto, ka maka ki roto ki te 37 ℃ kaukau wai mo te neke atu i te 30min, centrifuged, ka whakamahia te serum o runga mo te tatari.
2. potae karaka o te ngongo serum tere
Kei roto i nga oko kohi toto nga kaitao hei whakatere ake i te mahi whakakoi.Ka taea e te ngongo serum tere te whakakotahi i te toto kua kohia i roto i te 5 meneti.He pai mo te raupapa o nga whakamatautau serum ohorere.Koia te nuinga o te whakamahi i te coagulation e whakatairanga ana i te ngongo whakamatautau mo te koiora o ia ra, te aukati, te serum, nga homoni, me etahi atu i muri i te tuhi toto, ka taea te huri me te whakauru mo nga wa 5-8.A, no te he iti te pāmahana rūma, ka taea te hoatu ki roto ki te 37 ℃ pati wai mo te 10-20min, a ka taea te centrifuged te serum runga mo te tūtatari.
3. te uhi mahunga koura o te ngongo whakakorikori ira wehe
I taapirihia te reera me te coagulant ki te oko toto.I mau tonu te tauira i roto i te 48 haora i muri i te centrifugation.Ka taea e te coagulant te tere te whakahohe i te tikanga coagulation me te whakatere i te tukanga coagulation.Ko te momo tauira he serum, e tika ana mo nga whakamatautau koiora me nga whakamatautau pharmacokinetic.I muri i te kohinga, uruhia ki raro mo nga wa 5-8, tu tika mo te 20-30min, ka centrifuge te supernatant hei whakamahi.
4. potae pango o te konutai citrate ESR ngongo whakamatautau
Ko te kukū o te konutai citrate e hiahiatia ana mo te whakamatautau ESR he 3.2% (he rite ki te 0.109mol/l), me te tauwehenga o te anticoagulant ki te toto ko te 1:4.Kei roto 0.4ml o te 3.8% konutai citrate.Utu toto ki te 2.0ml.He ipuipu whakamatautau motuhake tenei mo te tere parataiao erythrocyte.Ko te momo tauira he plasma.He pai mo te tere parataiao erythrocyte.I muri i te tohanga toto, ka huri tonu, ka konatunatua mo nga wa 5-8.Ruperupea pai i mua i te whakamahi.Ko te rereketanga i waenga i taua mea me te ipuipu mo te whakamatautau take coagulation he rereke te kukū o te anticoagulant ki te rahinga o te toto, e kore e taea te rangirua.
5. konutai citrate coagulation ngongo whakamātautau potae kahurangi marama
Ko te konutai citrate he mahi anticoagulant te nuinga ma te chelating me nga katote konupūmā i roto i nga tauira toto.Ko te kukū o te anticoagulant e taunakihia ana e te Komiti Motu mo te paerewa taiwhanga haumanu he 3.2% ranei 3.8% (he rite ki te 0.109mol/l, 0.129mol/l ranei), me te tauwehenga o te anticoagulant ki te toto he 1:9.Kei roto i te ipu kohinga toto korehau he 0.2ml o te 3.2% konutai citrate anticoagulant.Ka kohia te toto ki te 2.0ml.Ko te momo whakarite tauira he toto katoa, he plasma ranei.I muri i te kohinga, ka huri tonu, ka konatunatua mo nga wa 5-8.I muri i te centrifugation, ka tangohia te plasma o runga mo te tatari.He pai mo te whakamatautau coagulation, Pt, APTT me te whakamatautau take coagulation.
6. heparin anticoagulation ngongo potae matomato
I taapirihia te Heparin ki te oko toto.Ko te Heparin te paanga o te antithrombin tika, ka taea te whakaroa i te wa o te coagulation o nga tauira.Ka whakamahia i roto i nga mahi ohorere me te nuinga o nga whakamatautau koiora, penei i te mahi ate, te mahi whatukuhu, te toto toto, te huka toto, me etahi atu. E tika ana ki te whakamatautau ngoikore o te toto toto whero, te tātari hau toto, te whakamatautau hematocrit, te ESR me te whakatau tikanga koiora, kaore. he pai mo te whakamatautau hemagglutination.Ko te nui o te heparin ka taea te whakahiato leukocyte kaore e taea te whakamahi mo te tatau leukocyte.Kare e pai mo te whakarōpū leukocyte na te mea ka taea e ia te hanga i te papamuri o te poro toto kia tae ki te kikorangi marama.Ka taea te whakamahi mo te hemorheology.Ko te momo tauira he plasma.I muri tonu i te kohinga toto, whakahurihia ka uru mo nga wa 5-8.Tangohia te plasma o runga mo te tatari.
7. te uhi mahunga matomato marama o te ngongo wehenga plasma
Ko te taapiri i te heparin lithium anticoagulant ki roto i te ngongo wehe ka taea te whakatutuki i te kaupapa o te wehenga plasma tere.Ko te whiriwhiri pai rawa atu mo te kimi hiko.Ka taea hoki te whakamahi mo te tirotiro i te koiora plasma me te rapunga koiora plasma ohorere penei i te ICU.Ka whakamahia i roto i nga mahi ohorere me te nuinga o nga whakamatautau koiora, penei i te mahi ate, te mahi whatukuhu, te toto toto, te huka toto, me etahi atu. Ka taea te whakanoho tika i nga tauira plasma ki runga i te miihini ka noho pumau mo te 48 haora i raro i te rokiroki makariri.Ka taea te whakamahi mo te hemorheology.Ko te momo tauira he plasma.I muri tonu i te kohinga toto, whakahurihia ka uru mo nga wa 5-8.Tangohia te plasma o runga mo te tatari.
8. pāhare pāporo oxalate / konutai pūkōwhai pōtae hina
Ko te konutai fluoride he anticoagulant ngoikore.I te nuinga o te wa ka whakamahia tahi me te konutai oxalate me te konutai ethyliodate.Ko te wahanga he 1 te waahanga o te konutai pūkōwhai me te 3 ngā wāhanga o te pāhare pāporo oxalate.Ka taea e te 4mg o tenei ranunga te aukati i te 1ml o te toto mai i te whakakoi me te aukati i te pirau huka i roto i nga ra 23.Kaore e taea te whakamahi mo te whakatau Urea ma te tikanga Urease, mo te phosphatase alkaline me te amylase whakatau.E taunaki ana kia kitea te huka toto.Kei roto i te konutai fluoride, te pāhare pāporo oxalate, te rehu EDTA Na ranei, ka taea te aukati i te ngohe enolase i roto i te huka toto.I muri i te tohanga toto, ka hurihia, ka konatunatua mo nga wa 5-8.I muri i te centrifugation, ka tangohia te supernatant me te plasma mo te tatari.He ngongo motuhake mo te whakatau tere i te huka toto.
9. EDTA paipa anticoagulation potae papura
Ko te waikawa Ethylenediaminetetraacetic (EDTA, te taumaha molecular 292) me tona tote he momo waikawa polycarboxylic amino, e tika ana mo nga whakamatautau hematology whanui.Koia te ipuipu whakamatautau pai mo nga mahinga toto, hemoglobin glycosylated me nga whakamatautau roopu toto.Kaore e pa ana ki te whakamatautau coagulation me te whakamatautau mahi platelet, me te whakatau i te katote konupūmā, te katote konutai, te katote konutai, te katote rino, te phosphatase alkaline, te creatine kinase me te leucine aminopeptidase.He pai mo te whakamatautau PCR.Mapuhia te 100ml o te otinga 2.7%edta-k2 ki te pakitara o roto o te ngongo korehau, whakamaroke ki te 45 ℃, tangohia te toto ki te 2mi, ka huri tonu ka uru mo nga wa 5-8 i muri i te tohanga toto, katahi ka whakaranuhia hei whakamahi.Ko te momo tauira he toto katoa, me uru ina whakamahia.
Wā tuku: Hune-29-2022
