Umuyoboro wo gukusanya amaraso ya Vacuum ni umuvuduko ukabije wumuvuduko wa vacuum ikirahure gishobora kumenya gukusanya amaraso.Igomba gukoreshwa hamwe nurushinge rwo gukusanya amaraso.
Ihame ryo gukusanya amaraso
Ihame ryo gukusanya amaraso ya vacuum ni ugukurura umuyoboro wamaraso hamwe numutwe wumutwe muburyo butandukanye bwa vacuum mbere, ugakoresha umuvuduko wacyo mubi kugirango uhite ukusanya umubare wamaraso wamaraso, hanyuma winjize uruhande rumwe rwurushinge rwo gukusanya amaraso mumitsi yumuntu kandi ikindi kirangirira mumashanyarazi ya vacuum yo gukusanya amaraso.Amaraso yimitsi yumuntu ari mumitsi yo gukusanya amaraso.Mubikorwa byumuvuduko mubi, bijugunywa mubintu byintangarugero byamaraso binyuze murushinge rwo gukusanya amaraso.Munsi ya venipuncture, gukusanya imiyoboro myinshi irashobora kugerwaho nta kumeneka.Ingano ya lumen ihuza urushinge rwo gukusanya amaraso ni nto cyane, bityo ingaruka ziterwa no gukusanya amaraso zirashobora kwirengagizwa, ariko amahirwe yo guhura ni make.Kurugero, ingano ya lumen izatwara igice cyu cyuho cyumuvuduko wamaraso, bityo bigabanye ubwinshi bwo gukusanya.
Gutondekanya imiyoboro y'amaraso
Nkuko bigaragara ku gishushanyo cya 1, hari ubwoko 9 bwimitsi yo gukusanya amaraso ya vacuum, ishobora gutandukanywa ukurikije ibara ryigifuniko.
Igishushanyo 1 ubwoko bwimitsi yo gukusanya amaraso
1. serumu isanzwe ya capa itukura
Umuyoboro wo gukusanya amaraso urimo inyongeramusaruro, nta anticoagulant na procoagulant, gusa vacuum.Ikoreshwa muburyo busanzwe bwa serumu biochemie, banki yamaraso hamwe nibizamini bijyanye na serologiya, ibizamini bitandukanye bya biohimiki na immunologiya, nka sifilis, umubare wa hepatite B, nibindi ntibikeneye kunyeganyega nyuma yo gushushanya amaraso.Ubwoko bwikitegererezo ni serumu.Nyuma yo gushushanya amaraso, ashyirwa muri 37 bath ubwogero bwamazi burenze 30min, centrifuged, na serumu yo hejuru ikoreshwa muguhagarara.
2. orange cap ya serumu yihuta
Hariho coagulants mumitsi yo gukusanya amaraso kugirango yihutishe inzira ya coagulation.Umuyoboro wa serumu wihuse urashobora guhuza amaraso yakusanyijwe muminota 5.Birakwiriye kurukurikirane rwibizamini bya serumu byihutirwa.Nibisanzwe bikoreshwa cyane mugutezimbere ibizamini bya biohimiki ya buri munsi, ubudahangarwa, serumu, imisemburo, nibindi nyuma yo gushushanya amaraso, birashobora guhindurwa no kuvangwa inshuro 5-8.Iyo ubushyuhe bwicyumba buri hasi, burashobora gushirwa muri 37 bath amazi yogeramo 10-20min, kandi serumu yo hejuru irashobora gushirwa hamwe kugirango ihagarare.
3. zahabu yumutwe wa inert itandukanya gel yihuta
Inert gel na coagulant byongewe mumitsi yo gukusanya amaraso.Icyitegererezo cyagumye gihamye mumasaha 48 nyuma ya centrifugation.Coagulant irashobora gukora byihuse uburyo bwa coagulation kandi byihutisha inzira ya coagulation.Ubwoko bw'icyitegererezo ni serumu, ikwiranye n'ibizamini byihutirwa bya biohimiki na farumasi.Nyuma yo gukusanya, vanga hejuru inshuro 5-8, uhagarare neza kuri 20-30min, hanyuma centrifuge ndengakamere kugirango ikoreshwe.
4. umupira wumukara wa sodium citrate ESR yipimisha
Ubushuhe bukenewe bwa sodium citrate kugirango bipimishe ESR ni 3,2% (bihwanye na 0,109mol / l), naho igipimo cya anticoagulant n'amaraso ni 1: 4.Ifite 0.4ml ya 3,8% ya citrate ya sodium.Kuramo amaraso kuri 2.0ml.Numuyoboro udasanzwe wo gupima igipimo cya erythrocyte.Ubwoko bw'icyitegererezo ni plasma.Irakwiriye igipimo cya erythrocyte.Nyuma yo gushushanya amaraso, ihita ihindurwa ikavangwa inshuro 5-8.Shyira neza mbere yo gukoresha.Itandukaniro riri hagati yacyo nigitereko cyo kwipimisha coagulation factor ni uko kwibumbira hamwe kwa anticoagulant bitandukanye n’igipimo cy’amaraso, kidashobora kwitiranywa.
5. sodium citrate coagulation test tube yoroheje yubururu
Sodium citrate igira uruhare mu kurwanya antikagulant cyane cyane mu gushonga hamwe na calcium ion mu maraso.Ubwinshi bwa anticoagulant bwasabwe na komite yigihugu ishinzwe gupima laboratoire ni 3.2% cyangwa 3.8% (bihwanye na 0.109mol / l cyangwa 0.129mol / l), naho igipimo cya anticoagulant n'amaraso ni 1: 9.Umuyoboro w'amaraso wa vacuum urimo 0.2ml ya 3.2% ya sodium citrate anticoagulant.Amaraso yakusanyirijwe kuri 2.0ml.Ubwoko bw'icyitegererezo ni amaraso yose cyangwa plasma.Nyuma yo gukusanya, ihita ihindurwa ikavangwa inshuro 5-8.Nyuma ya centrifugation, plasma yo hejuru ifatwa kugirango ihagarare.Irakwiriye kwipimisha coagulation, Pt, APTT hamwe na coagulation factor test.
6. heparin anticoagulation tube icyatsi kibisi
Heparin yongerewe mu cyombo cyo gukusanya amaraso.Heparin ifite ingaruka za antithrombine mu buryo butaziguye, ishobora kongera igihe cyo guteranya ingero.Ikoreshwa mubushakashatsi bwihutirwa kandi bwinshi bwibinyabuzima, nkibikorwa byumwijima, imikorere yimpyiko, lipide yamaraso, glucose yamaraso, nibindi. ibereye ikizamini cya hemagglutination.Heparine ikabije irashobora gutera leukocyte guteranya kandi ntishobora gukoreshwa mukubara leukocyte.Ntabwo ibereye kurwego rwa leukocyte kuko irashobora gukora inyuma yibice byamaraso bisize ubururu bwerurutse.Irashobora gukoreshwa muri hemorheologiya.Ubwoko bw'icyitegererezo ni plasma.Ako kanya nyuma yo gukusanya amaraso, subiza hanyuma ubivange inshuro 5-8.Fata plasma yo hejuru kugirango uhagarare.
7. icyatsi kibisi gitwikiriye icyuma gitandukanya plasma
Ongeramo heparin lithium anticoagulant muri inert yo gutandukanya inert irashobora kugera ku ntego yo gutandukana byihuse plasma.Nuburyo bwiza bwo kumenya electrolyte.Irashobora kandi gukoreshwa muburyo busanzwe bwa plasma biochemical detection no gutahura byihutirwa plasma biochemiki nka ICU.Ikoreshwa mubyihutirwa kandi byinshi mubushakashatsi bwibinyabuzima, nkibikorwa byumwijima, imikorere yimpyiko, lipide yamaraso, glucose yamaraso, nibindi. Plasma irashobora gushirwa kumashini kandi igakomeza guhagarara mumasaha 48 mububiko bukonje.Irashobora gukoreshwa muri hemorheologiya.Ubwoko bw'icyitegererezo ni plasma.Ako kanya nyuma yo gukusanya amaraso, subiza hanyuma ubivange inshuro 5-8.Fata plasma yo hejuru kugirango uhagarare.
8. potasiyumu oxalate / sodium fluoride imvi
Sodium fluoride ni anticagulant idakomeye.Ubusanzwe ikoreshwa ifatanije na potassium oxalate cyangwa sodium etyliodate.Umubare ni igice cya fluoride ya sodium nibice 3 bya potasiyumu oxyde.4mg y'uruvange rushobora kubuza 1ml y'amaraso gutembera no kubuza isukari kwangirika muminsi 23.Ntishobora gukoreshwa muguhitamo urea nuburyo bwa Urease, cyangwa kuri fosifata ya alkaline na kugena amylase.Birasabwa kumenya amaraso glucose.Ifite sodium fluoride, potassium oxalate cyangwa EDTA Na spray, ishobora kubuza ibikorwa bya enolase muri metabolism glucose.Nyuma yo gushushanya amaraso, irahindurwa ikavangwa inshuro 5-8.Nyuma ya centrifugation, supernatant na plasma bifatwa kugirango bihagarare.Numuyoboro udasanzwe wo kumenya byihuse glucose yamaraso.
9. EDTA anticoagulation umuyoboro wumutuku
Acide Ethylenediaminetetraacetic (EDTA, uburemere bwa molekile 292) n'umunyu wacyo ni ubwoko bwa aside amine polyakarubisike, ibereye kwipimisha rusange.Nibikoresho byatoranijwe byo kwisuzumisha kumaraso, glycosylated hemoglobine no gupima amatsinda.Ntabwo ikoreshwa mubizamini bya coagulation hamwe na test ya mikorere ya platel, cyangwa no kumenya calcium ion, potasiyumu ion, sodium ion, ion ion, fosifatike ya alkaline, kinase kinase na aminopeptidase ya leucine.Birakwiriye kwipimisha PCR.Koresha 100ml ya 2,7% yumuti wa edta-k2 kurukuta rwimbere rwumuyoboro wa vacuum, uhumure kuri 45 ℃, fata amaraso kuri 2mi, uhite uhindukira hanyuma ubivange inshuro 5-8 nyuma yo gushushanya amaraso, hanyuma ubivange kugirango bikoreshe.Ubwoko bw'icyitegererezo ni amaraso yose, akeneye kuvangwa iyo akoreshejwe.
Igihe cyo kohereza: Jun-29-2022
