Li-syringe tse lahliloeng - Sehlomathiso 1
Ho lokisoa ha tharollo ea masala a ethylene oxide
0.1mol/L hydrochloric acid: hlapolla 9ml hydrochloric acid ho isa ho 1000ml.
0.5% tharollo ea nako: bekha 0.5g ea nako le ho hlapolla ho 100ml.
Tharollo ea sodium thiosulfate: bekha 1g ea sodium thiosulfate 'me u hlapolle ho 100ml.
10% sodium sulfite solution: boima ba 10. 0g anhydrous sodium sulfite, e qhale 'me u e hlapolle ho 100ml.
Tharollo ea tlhahlobo ea Magenta sulfurous acid: bekha 0.1g ea fuchsin ea mantlha ebe u eketsa 120ml ea metsi a chesang ho e qhaqha.Ka mor'a ho pholile, eketsa 20ml ea 10% ea sodium sulfite solution le 2ml ea hydrochloric acid lefifing.Tharollo ea teko e lokela ho ba e se nang 'mala.Haeba e fumanoa e le khubelu, e lokela ho lokisoa hape.
Ethylene glycol standard stock solution: nka botlolo e ommeng le e hloekileng ea 50ml ea volumetric, eketsa metsi a ka bang 30ml, e lekanya ka nepo, nka 0.5ml ethylene glycol, e kenye ka potlako ka botlolong, e sisinye, e lekanye, phapang pakeng tsa litekanyo tse peli ke boima bo ka har'a tharollo, eketsa metsi sekaleng, kopanya, 'me u bale mahloriso a eona ho latela mokhoa o latelang:
C＝W/50 × sekete se le seng
C: Glycol standard stock solution concentration g/l;
W: Boima ba ethylene glycol ka tharollo g
Tharollo e tloaelehileng ea Ethylene glycol: ka nepo nka 1.0ml ea tharollo e tloaelehileng ea setoko, 'me u hlapolle ho 1000ml ka metsi.
Ho lokisa reagent e nang le oxidized habonolo:
a.Hlakola sulfuric acid (20%): Nka H2SO4128ml, butle-butle ente 500mL, 'me u hlapolle ho 1000mL ka mor'a ho pholile.
b.Letšoao la starch: nka 0.5g ea starch e qhibilihang, e qhibilihe ka 100ml ea metsi, futhumatsa 'me ue belise, ebe u e pholile bakeng sa boemo ba ho ema (e tla be e lokiselitsoe ha e sebelisoa ka nakoana)
c.Tharollo ea potassium permanganate: nka 3.3gKMnO4, eketsa 1050ml ea metsi, pheha bakeng sa 15min, eketsa metsi ho 1050ml, e tlohele hore e eme matsatsi a fetang a mabeli ka mor'a ho tiisoa, e sefa ka khalase e nyenyane ea khalase, e sisinye, 'me u lekanye khatello ea eona.
Tekanyetso: Nka 0.25g ea referense ea sodium oxalate e omisitsoeng ho boima bo sa feleng ho 1050C, e lekanya ka nepo, eketsa 100ml ea tharollo ea sulfuric acid (8 + 92) 'me u hlohlelletse ho e qhaqha.Ka potlako eketsa 25ml ea tharollo e tloaelehileng ea KMnO4 hore e behoe ka tharollo ho tsoa ho buret.Ka mor'a ho fela, e chese ho 650C 'me u tsoele pele ho titrating ho fihlela tharollo e fetoha khubelu' me e lula e sa fetohe bakeng sa 30s.Qetellong ea titration, mocheso oa tharollo ha oa lokela ho ba ka tlase ho 550C, mme tlhahlobo e se nang letho e tla etsoa ka nako e le 'ngoe.6.7mg e 'ngoe le e' ngoe ea sodium oxalate e lekana le 1ml ea 0.02mol/L KMnO4 tharollo e tloaelehileng.Motsoako oa 'nete oa tharollo ena o baloa ho latela tšebeliso ea tharollo ena le palo ea sodium oxalate e nkiloeng.
d.C (KMnO4)=0.002 mol/L: Nka 0.02 mol/L KMnO4 ebe u eketsa metsi ho a hlapolla ka makhetlo a 10 pele o sebelisoa.
e.Sodium thiosulfate (0.1 mol/L): boima ba 26g Na2S2O3.5H2O kapa 16g anhydrous Na2S2O3, eketsa 0.2g anhydrous sodium carbonate, e qhibilihe ka metsing a 1000ml, butle-butle e belise bakeng sa 10min, e pholise, e tšele metsi a mabeli ho isa ho 10. libeke, 'me u lekanye mohopolo oa eona.
Calibration: boima ba 0.18g ea potassium dichromate e omisitsoeng ho 1200C ho boima bo sa feleng.Beha boima ka nepo.E kenye ka botlolo ea tekanyo ea iodine, e qhibilihe ka 25ml ea metsi, eketsa 2g ea potassium iodide le 20ml ea dilute sulfuric acid (20%), sisinya hantle, e behe sebakeng se lefifi bakeng sa 10min, eketsa 150ml ea metsi, titrate e. ka tharollo e lokiselitsoeng ea Na2S2O3 [c (Na2S2O3) ＝ 0.1 mol/L], eketsa 2ml ea tharollo ea starch indicator (10g/L) qetellong, tsoela pele ho reketla ho fihlela tharollo e fetoha ho tloha ho boputsoa ho ea ho botala bo khanyang, 'me u etse tlhahlobo e se nang letho ka nako yona eo.1ml e 'ngoe le e' ngoe ea Na2S2O3 (0.1 mol/L) e lekana le 4.9031mg ea potassium dichromate.Motsoako oa tharollo ena o baloa ho latela tšebeliso ea tharollo ena le palo ea potassium dichromate e nkiloeng.
f.C (Na2S2O3) ＝ 0.01 mol/L: hlapolla 0.1 mol/L Na2S2O3 ka metsi a sa tsoa belisoa le a pholileng pele o ema ka makhetlo a 10.
Nako ea poso: Oct-04-2022
