Qhov sib txawv ntawm cov concentrated gel thiab cais gel
Tus nqi pH ntawm cov concentrated gel txawv ntawm qhov sib cais gel.Cov qub feem ntau qhia cov nyhuv concentration, thaum tom kawg qhia tau hais tias cov nyhuv thiab molecular sieve nyhuv.Cov nyhuv concentration yog ua tiav nyob rau hauv cov concentrated gel.Lub pH ntawm cov concentrated gel yog 6.8.Raws li qhov pH mob no, yuav luag tag nrho Cl ions ntawm HCl hauv qhov tsis sib xws, thiab qhov isoelectric point ntawm Gly yog 6.0.Tsuas yog ob peb qhov sib txawv hauv qhov tsis zoo ions, uas txav qeeb heev hauv qhov hluav taws xob.Acidic proteins yog dissociated rau hauv tsis zoo ions ntawm no pH, thiab cov migration tus nqi ntawm peb hom ions yog cl> General proteins> Gly.Tom qab electrophoresis pib, Cl ions txav nrawm, tawm hauv qhov chaw qis ion concentration tom qab.Gly txav qeeb heev hauv qhov hluav taws xob, uas ua rau tsis muaj kev txav ions, yog li thaj tsam high-voltage tsis muaj ions yog tsim ntawm ions ceev thiab qeeb.Tag nrho cov ions tsis zoo nyob rau hauv cheeb tsam high-voltage yuav ua kom lawv txav mus los.Thaum lawv tsiv mus rau thaj tsam Cl ion, qhov hluav taws xob siab ploj mus, thiab qhov txav ceev ntawm cov protein qeeb.Tom qab lub xeev ruaj khov saum toj no tau tsim, cov qauv ntawm cov protein yog concentrated ntawm cov ions nrawm thiab qeeb los ua ib qho nqaim interlayer, Nws tau teem rau hauv cov hlua raws li tus nqi tsis zoo nqa los ntawm cov protein.Tom qab cov qauv concentrated nkag mus rau hauv kev sib cais gel los ntawm cov concentrated gel, pH ntawm cov gel nce, qhov dissociation degree ntawm Gly nce, thiab kev txav mus los.Ntxiv mus, vim nws cov molecule me me, nws tshaj tag nrho cov protein molecules.Tam sim ntawd tom qab Cl ions tsiv teb tsaws, qhov qis ion concentration tsis muaj lawm, ua rau lub zog hluav taws xob tas li.Yog li ntawd, kev sib cais ntawm cov qauv ntawm cov protein nyob rau hauv kev sib cais gel feem ntau yog nyob ntawm nws cov nqi zog, molecular loj thiab cov duab.Qhov pore loj ntawm kev sib cais gel muaj qee qhov loj me.Rau cov proteins nrog cov txheeb ze sib txawv, cov nyhuv hysteresis tau txais thaum dhau qhov sib txawv.Txawm hais tias cov khoom uas muaj qhov sib npaug zoo li qub yuav cais cov protein ntau qhov sib txawv ntawm ib leeg vim yog cov txiaj ntsig ntawm no molecular sieve.
Qhov sib txawv ntawm 10% thiab 12% ntawm cov kua nplaum sib cais
Raws li qhov hnyav molecular ntawm koj lub hom phiaj protein, yog tias nws yog cov protein uas muaj qhov hnyav dua molecular (siab dua 60KD), koj tuaj yeem siv 10% kua nplaum, yog tias nws yog cov protein uas muaj qhov hnyav ntawm 60 thiab 30kd, koj tuaj yeem siv 12. % kua nplaum, thiab yog tias nws qis dua 30kd, feem ntau kuv siv 15% kua nplaum.Lub ntsiab lus tseem ceeb yog tias thaum qhov taw qhia kab cia li khiav tawm ntawm cov roj hmab hauv qab, koj lub hom phiaj protein tuaj yeem nyob hauv nruab nrab ntawm cov roj hmab.
Qhov pore loj ntawm gel sib xws rau qhov sib txawv ntawm cov gel kuj txawv.Lub pore loj nrog me me concentration yog loj, thiab lub pore loj nrog loj concentration yog me me.Feem ntau, cov gel sib cais yog 12% thiab cov concentrated gel yog 5%, vim hais tias lub hom phiaj ntawm cov concentrated gel yog kom mloog zoo rau tag nrho cov proteins ntawm tib txoj kab pib, thiab tom qab ntawd nkag mus rau kev sib cais gel rau kev sib cais.Nyob ntawm qhov loj ntawm cov protein.
Post lub sij hawm: Sep-05-2022
