Umahluko phakathi kwejeli egxininisiweyo kunye nejeli yokwahlukana
Ixabiso le-pH lejeli eligxininisiweyo lihluke kwijeli yokwahlukana.Eyangaphambili ibonisa ubukhulu becala isiphumo sokugxininiswa, ngelixa le yokugqibela ibonisa umphumo wentlawulo kunye nefuthe le-molecular sieve.Umphumo wogxininiso ugqitywe ngokukodwa kwi-gel egxininisiweyo.I-pH yejeli egxininisiweyo yi-6.8.Ngaphantsi kwale meko ye-pH, phantse zonke ii-Cl ions ze-HCl kwi-buffer zihlukanisiwe, kwaye indawo ye-isoelectric ye-Gly yi-6.0.Bambalwa kuphela abahlulwe kwii-ion ezingalunganga, ezihamba kancinci kakhulu kwindawo yombane.Iiprotheyini ezineasidi ziyahlulwa zibe yi-ion ezingalunganga kule pH, kwaye izinga lokufuduka kwezi ntlobo zintathu zee-ion yi-cl > Iiproteni ngokubanzi > i-Gly.Emva kokuba i-electrophoresis iqalile, ii-Cl ions zihamba ngokukhawuleza, zishiya indawo ephantsi yoxinaniso lwe-ion ngasemva.I-Gly ihamba ngokucothayo kumhlaba wombane, okukhokelela ekunqongophelweni kwee-ion ezihambayo, ngoko ke ummandla one-voltage ephezulu engenazo ii-ion kwakheka phakathi kweeyoni ezikhawulezayo nezicothayo.Zonke ii-ion ezingalunganga kwingingqi ye-voltage ephezulu ziya kukhawulezisa ukuhamba kwazo.Xa befudukela kummandla weCl ion, i-voltage ephezulu iyanyamalala, kwaye isantya esihambayo seprotheni siyancipha.Emva kokumiselwa kwesi sikhundla singentla, isampuli yeprotheyini igxininiswe phakathi kwe-ion ekhawulezayo kunye necothayo ukwenza i-interlayer emxinwa, Icwangciswe ibe ngamabhanti ngokwexabiso lentlawulo engalunganga eqhutywa yiprotheni.Emva kokuba isampuli egxininisiweyo ingene kwi-gel yokwahlula kwi-gel egxininisiweyo, i-pH ye-gel iphakama, i-dissociation degree ye-Gly yanda, kwaye ukuhamba kuphakama.Ngaphezu koko, ngenxa yokuba imolekyuli yayo incinane, yodlula zonke iimolekyuli zeprotini.Ngokukhawuleza emva kokuba i-Cl ions ifuduka, i-ion concentration ephantsi ayisekho, isenza amandla ombane angatshintshiyo.Ngoko ke, Ukwahlulwa kweesampulu zeprotheni kwijeli yokwahlula ubukhulu becala kuxhomekeke kwiipropati zayo zentlawulo, ubungakanani bemolekyuli kunye nokumila.Ubungakanani bepore yegel yokwahlula bunobungakanani obuthile.Kwiiprotheni ezinobunzima obuhlukeneyo, umphumo we-hysteresis ofunyenwe xa udlula uhlukile.Nkqu amasuntswana aneentlawulo ezilinganayo ze-static ziya kwahlula iiprotheyini ezinobungakanani obuhlukeneyo omnye komnye ngenxa yesiphumo sale sieve yemolekyuli.
Umahluko phakathi kwe-10% kunye ne-12% yokwahlula iglue
Ngokobunzima bemolekyuli yeprotheni yakho ekujoliswe kuyo, ukuba iprotheni enesisindo esikhulu se-molecular (ngaphezulu kwe-60KD), ungasebenzisa i-glue ye-10%, ukuba iprotheni enobunzima be-molecular phakathi kwe-60 kunye ne-30kd, ungasebenzisa i-12 Iglue%, kwaye ukuba ingaphantsi kwe-30kd, ndidla ngokusebenzisa i-glue ye-15%.Inqaku eliphambili kukuba xa umgca wesalathisi uphuma nje kwi-rubber ephantsi, iprotheni yakho ekujoliswe kuyo ingaba phakathi kwerabha.
Ubungakanani bepore yejeli ehambelana nokugxilwa okuhlukeneyo kwejeli nayo yahlukile.Ubungakanani bepore kunye noxinzelelo oluncinci lukhulu, kwaye ubukhulu bepore kunye noxinzelelo olukhulu luncinci.Ngokuqhelekileyo, i-gel yokwahlula i-12% kwaye i-gel egxininisiweyo yi-5%, kuba injongo ye-gel egxininisiweyo kukugxininisa zonke iiprotheni kumgca wokuqala ofanayo, kwaye emva koko ufake i-gel yokwahlula ukuhlukana.Kuxhomekeke kubungakanani beprotheni.
Ixesha lokuposa: Sep-05-2022
