Phapang pakeng tsa gel e tsitsitseng le gel ea karohano

Phapang pakeng tsa gel e tsitsitseng le gel ea karohano

Theko ea pH ea gel e tsitsitseng e fapane le ea gel ea karohano.Ea pele e bonts'a haholo phello ea mahloriso, athe ea morao-rao e bonts'a phello ea tefiso le phello ea limolek'hule tsa sieve.Phello ea mahloriso e phethoa haholo-holo ka gel e tsitsitseng.pH ea gel e tsitsitseng ke 6.8.Tlas'a boemo bona ba pH, hoo e batlang e le li-Cl tsohle tsa HCl tse ka har'a buffer li arohane, 'me ntlha ea isoelectric ea Gly ke 6.0.Ke ba seng bakae feela ba arohaneng ka li-ion tse mpe, tse tsamaeang butle haholo tšimong ea motlakase.Liprotheine tse nang le asiti li aroloa ho ba li-ion tse mpe ho pH ena, 'me sekhahla sa ho falla ha mefuta e meraro ea li-ion ke cl > Liprotheine tse akaretsang > Gly.Ka mor'a hore electrophoresis e qale, li-ion tsa Cl li tsamaea ka potlako, li siea sebaka se tlaase sa ion ka morao.Gly e tsamaea butle haholo tšimong ea motlakase, e leng se bakang khaello ea li-ion tse tsamaeang, kahoo sebaka se phahameng sa motlakase se se nang li-ion se thehoa pakeng tsa li-ion tse potlakileng le tse liehang.Li-ion tsohle tse mpe sebakeng se phahameng sa motlakase li tla potlakisa motsamao oa tsona.Ha ba fallela sebakeng sa Cl ion, motlakase o phahameng oa nyamela, 'me lebelo le tsamaeang la protheine le fokotseha.Ka mor'a hore boemo bo tsitsitseng bo ka holimo bo thehoe, sampuli ea protheine e tsepamisitsoe pakeng tsa li-ion tse potlakileng le tse liehang ho theha interlayer e moqotetsane, E hlophisitsoe ka lihlopha ho ea ka tefiso e mpe e tsamaisoang ke protheine.Ka mor'a hore sampuli e tsitsitseng e kene ka gel ea karohano ho tloha gel e tsitsitseng, pH ea gel ea phahama, tekanyo ea ho arohana ea Gly e eketseha, 'me ho tsamaea hoa phahama.Ho feta moo, kaha molek’hule ea eona e nyenyane, e feta limolek’hule tsohle tsa protheine.Hang ka mor'a hore li-Cl ions li falle, khatello ea ion e tlaase ha e sa le teng, e etsa matla a kamehla a matla a motlakase.Ka hona, karohano ea disampole tsa protheine ka gel ea karohano e itšetlehile haholo ka thepa ea eona ea tefiso, boholo ba limolek'hule le sebōpeho.Boholo ba pore ea gel ea karohano bo na le boholo bo itseng.Bakeng sa liprotheine tse nang le boima bo fapaneng, phello ea hysteresis e fumanoang ha e feta e fapane.Esita le likaroloana tse nang le litefello tse lekanang tsa static li tla arola liprotheine tsa boholo bo fapaneng ho tse ling ka lebaka la phello ea sieve ena ea molek'hule.