Umehluko phakathi kwejeli egxilile kanye nejeli yokuhlukanisa
Inani le-pH lejeli eligxilile lihlukile kulelo lejeli lokuhlukanisa.Owokuqala ukhombisa ikakhulukazi umthelela wokugxilisa ingqondo, kanti lokhu kwakamuva kubonisa umphumela wokushaja kanye nomphumela we-molecular sieve.Umphumela wokuhlushwa ugcwaliswa ikakhulukazi kujeli eligxilile.I-pH yejeli egxilile ingu-6.8.Ngaphansi kwalesi simo se-pH, cishe wonke ama-Cl ions e-HCl ku-buffer ahlukanisiwe, futhi iphoyinti le-isoelectric le-Gly lingu-6.0.Bambalwa kuphela abahlukaniswa ama-ion angalungile, ahamba kancane kakhulu endaweni kagesi.Amaprotheni ane-asidi ahlukaniswa abe ama-ion angemahle kule pH, futhi izinga lokufuduka lezinhlobo ezintathu zama-ion i-cl > Amaprotheni Ajwayelekile > I-Gly.Ngemva kokuqala kwe-electrophoresis, ama-Cl ions ahamba ngokushesha, ashiye indawo yokuhlushwa ye-ion ephansi ngemuva.I-Gly ihamba kancane kakhulu emkhakheni kagesi, okuholela ekuntulekeni kwama-ion ahambayo, ngakho-ke isifunda esine-voltage ephezulu esintula ama-ion siyakhiwa phakathi kwama-ion asheshayo nahamba kancane.Wonke ama-ion ama-negative esifundeni se-high-voltage azosheshisa ukunyakaza kwawo.Lapho bethuthela esifundeni se-Cl ion, i-voltage ephezulu iyanyamalala, futhi ijubane elihambayo lamaprotheni liyancipha.Ngemva kokusungulwa kwesimo esingenhla esizinzile, isampula yephrotheni igxiliswa phakathi kwama-ion asheshayo futhi ahamba kancane ukuze kwakheke i-interlayer ewumngcingo, Ihlelwa ibe amabhande ngokuya ngenani lokushaja okungekuhle elithwalwa amaprotheni.Ngemuva kokuthi isampula egxilile ingene kujeli yokuhlukanisa kusukela kujeli egxilile, i-pH yejeli iyakhuphuka, izinga lokuhlukanisa le-Gly liyakhuphuka, futhi ukuhamba kuyakhuphuka.Ngaphezu kwalokho, ngenxa yokuthi i-molecule yayo incane, idlula wonke ama-molecule amaprotheni.Ngokushesha ngemva kokufuduka kwama-Cl ions, ukugxiliswa kwe-ion ephansi akusekho, okwenza amandla enkambu kagesi angashintshi.Ngakho-ke, Ukuhlukaniswa kwamasampula amaprotheni kujeli yokuhlukanisa ikakhulukazi kuncike ezintweni zayo zokushaja, usayizi wamangqamuzana nokuma.Usayizi we-pore wejeli yokuhlukanisa unobukhulu obuthile.Kumaprotheni anesisindo esihlobene esihlukile, umphumela we-hysteresis otholwe lapho udlula uhlukile.Ngisho nezinhlayiya ezinamashaji alinganayo amile zizohlukanisa amaprotheni anobukhulu obuhlukene komunye nomunye ngenxa yomphumela walesi sieve samangqamuzana.
Umehluko phakathi kuka-10% no-12% wokuhlukanisa iglue
Ngokwesisindo se-molecular of your target protein, uma kuyiprotheyini enesisindo esikhulu samangqamuzana (ngaphezu kuka-60KD), ungasebenzisa iglue engu-10%, uma kuyiprotheni enesisindo samangqamuzana phakathi kuka-60 no-30kd, ungasebenzisa i-12 % glue, futhi uma ingaphansi kuka-30kd, ngivame ukusebenzisa i-glue engu-15%.Iphuzu eliyinhloko liwukuthi lapho umugqa wenkomba uphuma nje ngaphansi kwerabha, iphrotheni yakho ohlosiwe ingaba maphakathi nerabha.
Usayizi we-pore wejeli ohambisana nokugxila okuhlukene kwejeli nawo uhlukile.Usayizi wembotshana onokugxiliswa okuncane mkhulu, futhi usayizi wembotshana onokugxila okukhulu mncane.Ngokuvamile, ijeli yokuhlukanisa i-12% futhi i-gel egxilile i-5%, ngoba inhloso yejeli eligxilile ukugxilisa wonke amaprotheni emgqeni ofanayo wokuqala, bese ufaka ijeli yokuhlukanisa ukuze uhlukanise.Kuye ngobukhulu beprotheni.
Isikhathi sokuthumela: Sep-05-2022
