Bambanci tsakanin maida hankali gel darabuwa gel
Ƙimar pH na gel mai mahimmanci ya bambanta da na gel na rabuwa.Tsohon yana nuna tasirin maida hankali, yayin da na karshen yana nuna tasirin caji da tasirin sieve kwayoyin.An ƙaddamar da sakamako mai mahimmanci a cikin gel mai mahimmanci.pH na gel mai da hankali shine 6.8.A ƙarƙashin wannan yanayin pH, kusan dukkanin Cl ions na HCl a cikin buffer suna
dissociated, kuma isoelectric batu na Gly ne 6.0.Kadan ne kawai aka rabu cikin ions mara kyau, waɗanda ke motsawa a hankali a cikin filin lantarki.Ana rarraba sunadaran acidic zuwa ions mara kyau a wannan pH, kuma yawan ƙaura na nau'ikan ions guda uku shine cl> Gabaɗaya sunadaran> Gly.Bayan electrophoresis ya fara, Cl ions suna tafiya da sauri, suna barin ƙananan ƙwayar ion a baya.Gly yana motsawa a hankali a cikin filin lantarki, yana haifar da rashin motsin ions, don haka an kafa yanki mai girma wanda ba shi da ions tsakanin sauri da jinkirin ions.Duk ions mara kyau a cikin babban ƙarfin wutar lantarki zai hanzarta motsi.Lokacin da suka matsa zuwa yankin Cl ion, babban ƙarfin lantarki ya ɓace, kuma saurin motsi na furotin yana raguwa.Bayan an kafa yanayin kwanciyar hankali na sama, samfurin sunadaran yana mai da hankali tsakanin ions mai sauri da jinkirin don samar da kunkuntar interlayer, An shirya shi cikin makada gwargwadon adadin cajin da furotin ke ɗauka.Bayan samfurin da aka mayar da hankali ya shiga cikin gel ɗin rabuwa daga gel mai daɗaɗɗa, pH na gel ya tashi, matakin rarraba Gly yana ƙaruwa, kuma motsi ya tashi.Haka kuma, saboda kwayoyin halittarsa karami ne, ya zarce dukkan kwayoyin gina jiki.Nan da nan bayan Cl ions sun yi ƙaura, ƙarancin ƙwayar ion ba ya wanzu, yana samar da ƙarfin filin lantarki akai-akai.Sabili da haka, Rabuwar samfuran furotin a cikin gel ɗin rabuwa ya dogara ne akan kaddarorin cajinsa, girman kwayoyin halitta da siffarsa.Girman pore na gel rabuwa yana da ƙayyadaddun girman.Don sunadaran da ke da nauyin dangi daban-daban, tasirin hysteresis da aka samu lokacin wucewa ya bambanta.Ko da barbashi tare da caji daidai gwargwado za su raba sunadaran masu girma dabam da juna saboda tasirin wannan sieve na ƙwayoyin cuta.
Bambanci tsakanin 10% da 12% na raba manne
Dangane da nauyin kwayoyin halitta na furotin da kuke so, idan yana da furotin mai girma da nauyin kwayoyin halitta (sama da 60KD), za ku iya amfani da 10% manne, idan furotin ne mai nauyin kwayoyin tsakanin 60 da 30kd, kuna iya amfani da 12. % manne, kuma idan ya yi ƙasa da 30kd, yawanci ina amfani da manne 15%.Babban ma'ana shine lokacin da layin nuna alama kawai ya fita daga ƙasan roba, furotin da kuke buƙata na iya kasancewa kawai a tsakiyar roba.
Girman pore na gel wanda ya dace da nau'i daban-daban na gel shima ya bambanta.Girman pore tare da ƙananan ƙaddamarwa yana da girma, kuma girman pore tare da babban taro yana da ƙananan.Gabaɗaya, gel ɗin rabuwa shine 12% kuma gel mai mai da hankali shine 5%, saboda manufar gel ɗin da aka tattara shine don tattara dukkan sunadaran akan layin farawa ɗaya, sannan shigar da gel ɗin rabuwa don rabuwa.Dangane da girman furotin.
Lokacin aikawa: Satumba-05-2022
