Musiyano pakati peiyo concentrated gel uyegel rekuparadzanisa
Iyo pH kukosha kweiyo yakadzika gel yakasiyana neiyo yekuparadzanisa gel.Iyo yekutanga inonyanya kuratidza maitiro ekugadzirisa, nepo iyo yekupedzisira inoratidza kubhadharisa mhedzisiro uye molecular sieve athari.Iyo yekusimbisa mhedzisiro inonyanya kupedzwa muiyo yakadzika gel.Iyo pH yejiro yakanyungudutswa ndeye 6.8.Pasi peiyi pH mamiriro, anenge ese maCl ions eHCl ari mubuffer ari
yakaparadzaniswa, uye isoelectric poindi yeGly ndeye 6.0.Vashomanana chete vanoparadzaniswa kuva ion dzisina kunaka, dzinofamba zvishoma nezvishoma mumunda wemagetsi.Mapuroteni ane acidic anopatsanurwa kuita maion asina kunaka papH iyi, uye mwero wekufamba kwemhando nhatu dzeiyoni ndeye cl > General proteins > Gly.Mushure mekunge electrophoresis yatanga, maCl ions anofamba nekukurumidza, achisiya nzvimbo yakaderera yeion concentration kumashure.Gly inofamba zvishoma nezvishoma mumunda wemagetsi, zvichiita kuti pave nekushaikwa kweioni dzinofamba, saka nzvimbo yakakwirira-voltage isina maion inoumbwa pakati peioni inokurumidza uye inononoka.Ioni dzese dzisina kunaka munzvimbo yepamusoro-voltage dzichamhanyisa kufamba kwavo.Pavanoenda kunharaunda yeCl ion, simba guru rinonyangarika, uye kukurumidza kufamba kweprotein kunoderera.Mushure mekunge nyika yakagadzikana iri pamusoro yasimbiswa, sampuli yeprotein inosungirirwa pakati pekukurumidza uye inononoka ions kuti iite interlayer yakamanikana, Inorongwa kuita mabhendi zvichienderana nehuwandu hwekuchajisa kwakashata kunotakurwa neprotein.Mushure mokunge sampu yakanyanyisa inopinda mujero rekuparadzanisa kubva kune gel yakanyanyisa, pH yejeri inokwira, dhigirii yekuparadzanisa yeGly inowedzera, uye kufamba kunokwira.Uyezve, nekuti morekuru rayo idiki, rinopfuura mapuroteni ese mamorekuru.Pakarepo mushure mokunge maCl ions atama, iyo yakaderera ion concentration haipo, ichigadzira simba remagetsi rinogara riripo.Naizvozvo, Kupatsanurwa kweprotein samples mune yekuparadzanisa gel kunonyanya kuenderana nekuchaja kwayo, saizi yemamorekuru uye chimiro.Iyo pore saizi yejeru yekuparadzanisa ine humwe hukuru.Kune mapuroteni ane hurema hwakasiyana, iyo hysteresis mhedzisiro inogamuchirwa kana ichipfuura yakasiyana.Kunyangwe zvimedu zvine akaenzana static charges zvichaparadzanisa mapuroteni ehukuru hwakasiyana kubva kune mumwe nekuda kwekuita kweiyi molecular sieve.
Musiyano pakati pe10% ne12% yekuparadzanisa glue
Zvinoenderana nehuremu hwemapuroteni eprotein yako yaunovavarira, kana iri puroteni ine huremu hwakakura (pamusoro pe60KD), unogona kushandisa 10% glue, kana iri protein ine huremu hwemamolecular pakati pe60 ne30kd, unogona kushandisa gumi nemaviri % glue, uye kana yakaderera pane 30kd, ini ndinowanzo kushandisa 15% glue.Chinhu chikuru ndechekuti kana mutsara wechiratidzo uchangobuda murabha pasi, chinangwa chako cheprotein chinogona kunge chiri pakati perabha.
Iyo pore saizi yegel inoenderana nekusiyana kwakasiyana gel kwakasiyanawo.Iyo pore saizi ine diki yekumisikidza yakakura, uye saizi yepore ine hombe concentration idiki.Kazhinji, gel rekuparadzanisa ndiro 12% uye gel yakanyanyisa ndeye 5%, nokuti chinangwa chejeri chakanyanyisa ndechekuisa mapuroteni ose pamutsara wakafanana wekutanga, uye wozopinda gel rekuparadzanisa kuti riparadzanise.Zvichienderana nehukuru hweprotein.
Nguva yekutumira: Sep-05-2022
