ʻO ka ʻokoʻa ma waena o ka gel concentrated a me ka separation gel

hoʻokaʻawale ʻia, a ʻo ka helu isoelectric o Gly he 6.0.He liʻiliʻi wale nō ka mea i hoʻokaʻawale ʻia i loko o nā ion maikaʻi ʻole, e neʻe mālie loa i ke kahua uila.Hoʻokaʻawale ʻia nā protein acid i loko o nā ion maikaʻi ʻole ma kēia pH, a ʻo ka neʻe ʻana o nā ʻano ion ʻekolu ʻo cl > General proteins > Gly.Ma hope o ka hoʻomaka ʻana o ka electrophoresis, neʻe wikiwiki nā ion Cl, e waiho ana i kahi ʻāpana haʻahaʻa haʻahaʻa ma hope.Holo mālie loa ʻo Gly ma ke kahua uila, no laila ka nele o nā ion neʻe, no laila ua hoʻokumu ʻia kahi ʻāpana kiʻekiʻe-voltage nele i nā ion ma waena o nā ion wikiwiki a me ka lohi.ʻO nā ion maikaʻi ʻole a pau i ka ʻāina kiʻekiʻe-voltage e hoʻolalelale i kā lākou neʻe.Ke neʻe lākou i ka ʻāpana Cl ion, nalowale ka uila kiʻekiʻe, a lohi ka neʻe ʻana o ka protein.Ma hope o ka hoʻokumu ʻia ʻana o ka mokuʻāina paʻa i luna, ua hoʻopaʻa ʻia ka hāpana protein ma waena o nā ion wikiwiki a me ka lohi e hana i kahi interlayer haiki, ua hoʻonohonoho ʻia i loko o nā pūʻulu e like me ka nui o ka uku maikaʻi ʻole i lawe ʻia e ka protein.Ma hope o ke komo ʻana o ka laʻana i loko o ka gel hoʻokaʻawale mai ka gel concentrated, piʻi ka pH o ka gel, piʻi ka degere dissociation o Gly, a piʻi ka mobility.Eia kekahi, no ka liʻiliʻi o kāna mole, ʻoi aku ia ma mua o nā molekole protein a pau.Ma hope koke o ka neʻe ʻana o nā ion Cl, ʻaʻole e noho hou ka ʻike ion haʻahaʻa, e hana ana i ka ikaika o ke kahua uila.No laila, ʻo ka hoʻokaʻawale ʻana o nā ʻāpana protein i ka separation gel e hilinaʻi nui ʻia i kāna mau waiwai, ka nui o ka molekula a me ke ʻano.ʻO ka nui o ka pore o ka gel hoʻokaʻawale i kekahi nui.No nā protein me ka nui pili like ʻole, ʻokoʻa ka hopena hysteresis i ka wā e hele ai.ʻO nā ʻāpana like ʻole me nā koina static e hoʻokaʻawale i nā protein o nā ʻano nui like ʻole ma muli o ka hopena o kēia sieve molecular.

ʻO ka ʻokoʻa ma waena o 10% a me 12% o ka hoʻokaʻawale ʻana i ke kāpili

E like me ke kaumaha molekala o kāu pūmua i manaʻo ʻia, inā he pūmua me ka paona molekala nui aʻe (ma luna o 60KD), hiki iā ʻoe ke hoʻohana i ka 10% glue, inā he protein me ke kaumaha molekala ma waena o 60 a me 30kd, hiki iā ʻoe ke hoʻohana i 12 % glue, a inā he haʻahaʻa ma mua o 30kd, hoʻohana pinepine au i ka 15% glue.ʻO ka manaʻo nui ʻo ia ke holo wale ka laina hōʻailona mai ka lalo o ka lāʻau lapaʻau, hiki i kāu protein i hoʻopaʻa ʻia ma waena wale nō o ka lāʻau.

ʻOkoʻa nō hoʻi ka nui pore o ka gel e pili ana i nā ʻokoʻa like ʻole o ka gel.ʻO ka nui o ka pore me ka liʻiliʻi liʻiliʻi he nui, a he liʻiliʻi ka nui o ka pore me ka nui.ʻO ka mea maʻamau, ʻo ka gel hoʻokaʻawale he 12% a ʻo ka gel concentrated he 5%, no ka mea, ʻo ke kumu o ka gel concentrated e hoʻopaʻa i nā protein āpau ma ka laina hoʻomaka hoʻokahi, a laila e komo i ka gel kaʻawale no ka hoʻokaʻawale ʻana.Ma muli o ka nui o ka protein.