Ọdịiche dị n'etiti gel gbadoro anya na gel nkewa

kewapụrụ, na isi ihe isoelectric nke Gly bụ 6.0.Naanị ole na ole na-ekewa n'ime ion na-adịghị mma, nke na-eji nwayọọ nwayọọ na-agagharị na mpaghara eletrik.A na-ekewa ndị na-edozi acidic n'ime ions na-adịghị mma na pH a, na ọnụego ọpụpụ nke ụdị ion atọ bụ cl> protein zuru ezu> Gly.Mgbe electrophoresis malitere, Cl ions na-aga ngwa ngwa, na-ahapụ ebe ịta ahụhụ dị ala n'azụ.Gly na-eji nwayọọ nwayọọ na-agagharị na mpaghara eletrik, na-akpata enweghị ion na-akpụ akpụ, ya mere a na-emepụta mpaghara voltaji na-enweghị ion n'etiti ngwa ngwa na ngwa ngwa.All ion na-adịghị mma na mpaghara voltaji dị elu ga-eme ka mmegharị ha dịkwuo elu.Mgbe ha na-aga na mpaghara Cl ion, nnukwu voltaji na-apụ n'anya, na-agba ọsọ nke protein na-ebelata.Mgbe ọnọdụ kwụsiri ike nke dị n'elu guzobechara, a na-etinye ihe nlele protein n'etiti ion ngwa ngwa na ngwa ngwa iji mepụta interlayer dị warara, a na-ahazi ya n'ime ụgbụ dị ka ọnụọgụ na-adịghị mma nke protein na-ebu.Mgbe ihe nlele ahụ na-abanye na gel nkewa site na gel na-etinye uche, pH nke gel na-ebili, ogo nkewa nke Gly na-abawanye, na mmegharị ahụ na-ebili.Ọzọkwa, n'ihi na molekul ya dị ntakịrị, ọ karịrị protein molecule niile.Ozugbo Cl ion kwagachara, ntinye ion dị ala adịghịzi adị, na-eme ike ọkụ eletrik mgbe niile.Ya mere, The nkewa nke protein samples na nkewa gel tumadi dabere na ya ụgwọ Njirimara, molekul size na udi.Ogo pore nke gel nkewa nwere ụfọdụ nha.Maka ndị na-edozi ahụ nwere oke dị iche iche, mmetụta hysteresis natara mgbe ị na-agafe dị iche.Ọbụna ụmụ irighiri ihe nwere ụgwọ static nha anya ga-ekewapụ protein nke nha dị iche iche na ibe ya n'ihi mmetụta nke sieve molecular a.

Ọdịiche dị n'etiti 10% na 12% nke ikewa gluu

Dị ka molekụla arọ nke protein gị lekwasịrị anya si dị, ọ bụrụ na ọ bụ protein nwere ibu molekụla buru ibu (n'elu 60KD), ị nwere ike iji gluu 10%, ma ọ bụrụ na ọ bụ protein nwere ịdị arọ molekụla n'etiti 60 na 30kd, ị nwere ike iji 12. % gluu, ma ọ bụrụ na ọ dị ala karịa 30kd, m na-ejikarị 15% gluu.Isi ihe bụ na mgbe akara akara na-apụ na rọba ala, protein ebumnuche gị nwere ike ịdị n'etiti rọba ahụ.

Ogo pore nke gel kwekọrọ na mkpokọta gel dị iche iche dịkwa iche.Ogo nke pore na obere ntinye dị ukwuu, na oghere nke nwere nnukwu ntinye dị ntakịrị.N'ozuzu, gel nkewa bụ 12% na gel na-etinye uche bụ 5%, n'ihi na nzube nke gel na-etinye uche bụ itinye uche na protein niile n'otu akara mmalite, wee banye gel nkewa maka nkewa.Dabere nha nke protein.