Amfanirabuwa manne
matsaloli na gama gari:
1) Girman tsakanin takarda tace, manne da membrane gabaɗaya takarda tace> = membrane> = manne.
2) Dole ne babu kumfa tsakanin takarda tace, manne da membrane, wanda zai haifar da gajeren kewayawa.
3) Saboda hydrophobicity na membrane, da membrane dole ne da farko a jika gaba daya a cikin methanol.Bugu da kari, fim din dole ne a jika a kowane lokaci a cikin aiki na gaba (sai dai hanyar bushewar fim).
4) Ana iya sake amfani da takarda tace.Akwai sunadaran da aka canjawa wuri da yawa a cikin takarda tace babba (membrane), don haka ba dole ba ne a haɗa takaddun tace sama da ƙasa.Idan ba za a iya bambanta su ba, ana iya maye gurbin takarda tace roba.
5) Lokacin canja wuri gaba ɗaya shine 1.5 hours, (1ma-2ma / cm2, 10% gel), kuma ana iya daidaita lokacin canja wuri da na yanzu gwargwadon nauyin kwayoyin.
1) Lokacin laminating fim, manne da takarda sandwiches, yi aiki a cikin babban farantin karfe tare da jujjuya fim buffer.Bayan tarawa, canja wurin ukun zuwa na'urar fim mai jujjuya a layi daya.Kada ku sake motsa su, saboda an kawar da yiwuwar kumfa gaba ɗaya ta hanyar aiki a cikin buffer, don haka babu buƙatar fitar da kumfa.
2) Game da lokacin canja wurin fim: 20 V don jujjuyawar bushewa kaɗan × 30min
(b) hanyar rigar
Mu ba ma amfani da wannan hanyar, don haka ba za mu gabatar da ita a nan ba.
C gano sakamako bayan metastasis
1. Rini mai manne
Bayan tabo da Coomassie shuɗi mai haske da canza launi tare da Destain, duba ko akwai sauran furotin akan manne don nuna tasirin canja wuri.
2. Fim Din
Akwai nau'ikan maganin rini iri biyu, mai jujjuyawa kuma ba za a iya juyawa ba.Abubuwan da za a iya juyewa sun haɗa da ponceau-s red, fastgreen FC, CPTs, da dai sauransu bayan rini da waɗannan rinayen, za a iya wanke pigment ɗin, kuma za a iya amfani da membrane don ƙarin bincike.Amma rini da ba za a iya jurewa ba, irin su Coomassie shuɗi mai haske, tawada Indiya, amido Black 10B, da sauransu, kuma ba za a iya amfani da tabo fim ɗin don ƙarin bincike ba.
Lokacin aikawa: Satumba-09-2022
