Koreshakole
imvugo n'ibibazo bisanzwe:
1) Ingano iri hagati yimpapuro ziyungurura, kole na membrane mubisanzwe ni impapuro zungurura> = membrane> = kole.
2) Ntabwo hagomba kubaho ibituba hagati yimpapuro zungurura, kole na membrane, bizatera uruziga rugufi.
3) Kubera hydrophobicity ya membrane, membrane igomba kubanza gutose rwose muri methanol.Mubyongeyeho, firime igomba guhora itose igihe cyose mubikorwa byakurikiyeho (usibye uburyo bwa firime yumye).
4) Akayunguruzo gashobora kongera gukoreshwa.Hano hari poroteyine nyinshi zoherejwe zamamaza mu mpapuro zo hejuru zo kuyungurura (membrane), bityo impapuro zo hejuru no hepfo zo kuyungurura ntizigomba kuvangwa.Niba bidashobora gutandukanywa, impapuro zungurura impapuro zishobora gusimburwa.
5) Igihe cyo kwimura muri rusange ni amasaha 1.5, (1ma-2ma / cm2, gel 10%), kandi igihe cyo kwimura nigihe gishobora guhinduka ukurikije uburemere bwa molekile.
1) Mugihe cyo kumurika firime, kole hamwe nimpapuro sandwiches, kora mumasahani manini hamwe na bffer ya firime.Nyuma yo gutondekanya, ohereza bitatu mubikoresho bya firime bizunguruka.Ntukongere kubimura ukundi, kuberako ibishoboka byavanyweho burundu mugukorera muri buffer, ntabwo rero bikenewe gutwara ibibyimba
2) Kubijyanye no kohereza firime: 20 V yo kuzenguruka igice cyumye × 30min
(b) uburyo butose
Ntabwo ahanini dukoresha ubu buryo, ntabwo rero tuzabimenyekanisha hano.
C kumenya ingaruka nyuma ya metastasis
1. Irangi
Nyuma yo gusiga hamwe na Coomassie nziza yubururu no gushushanya hamwe na Destain, reba niba hakiri proteine kuri kole kugirango bigaragaze ingaruka zo kwimurwa.
2. Irangi rya firime
Hariho ubwoko bubiri bwibisubizo byirangi, bihinduka kandi bidasubirwaho.Ihindurwa harimo ponceau-s umutuku, icyatsi kibisi cyihuta FC, CPTs, nibindi nyuma yo gusiga irangi hamwe naya marangi, pigment irashobora gukaraba, kandi membrane irashobora gukoreshwa mubisesengura.Ariko amarangi adasubirwaho, nka Coomassie yubururu buhebuje, wino yo mubuhinde, amido Black 10B, nibindi, hamwe na firime yanduye ntishobora gukoreshwa mubisesengura.
Igihe cyo kohereza: Nzeri-09-2022
