Jirinkewa gluu
utions na nkịtị nsogbu:
1) Ogo n'etiti akwụkwọ nzacha, gluu na akpụkpọ ahụ bụ akwụkwọ nzacha n'ozuzu> = akpụkpọ anụ> = gluu.
2) E kwesịghị inwe afụ n'etiti akwụkwọ nzacha, gluu na akpụkpọ anụ, nke ga-eme ka obere sekit.
3) N'ihi hydrophobicity nke akpụkpọ ahụ, akpụkpọ ahụ ga-ebu ụzọ mee mmiri kpamkpam na methanol.Tụkwasị na nke ahụ, a ghaghị idebe ihe nkiri ahụ na mmiri mgbe niile na ọrụ na-esote (ma e wezụga usoro ihe nkiri akọrọ).
4) Enwere ike iji akwụkwọ nzacha mee ihe ọzọ.Enwere ọtụtụ protein ndị ebufefere na mpempe akwụkwọ nzacha nke elu (membrane), yabụ na agaghị agwakọta akwụkwọ nzacha nke elu na nke ala.Ọ bụrụ na enweghị ike ịmata ha, enwere ike dochie akwụkwọ nzacha rọba.
5) Oge mbufe n'ozuzu bụ awa 1.5, (1ma-2ma / cm2, 10% gel), na oge mbufe na ugbu a nwere ike gbanwee dịka ịdị arọ nke molekul.
1) Mgbe ị na-agbanye ihe nkiri, gluu na sandwiches akwụkwọ, rụọ ọrụ na nnukwu efere na ihe ntanye ihe nkiri na-atụgharị.Mgbe ikpokọtachara, nyefee atọ ahụ na ngwaọrụ ihe nkiri na-atụgharị n'otu aka ahụ.Akwụghachila ha ọzọ, n'ihi na ekpochapụla ohere nke afụ kpamkpam site n'ịrụ ọrụ na nchekwa, n'ihi ya, ọ dịghị mkpa ịkwanye egosipụta.
2) Banyere oge mbufe ihe nkiri: 20 V maka ntụgharị akọrọ ọkara × 30min
(b) Usoro mmiri
Anyị anaghị eji usoro a eme ihe, yabụ anyị agaghị ewebata ya ebe a.
C njirimara mmetụta mgbe metastasis gasịrị
1. Ntinye nchara
Ka emechara ya na Coomassie na-acha anụnụ anụnụ na-acha anụnụ anụnụ ma na-agbanwe ya na Destain, hụ ma ọ ka nwere protein na gluu iji gosipụta mmetụta nke mbufe.
2. Ihe nkiri agba
E nwere ụdị ihe ngwọta abụọ dị iche iche nke agba agba agba, na-atụgharị na nke a na-apụghị ịgbagha agbagha.Ndị a na-atụgharị na-agụnye ponceau-s red, fastgreen FC, CPTs, wdg mgbe ejichara ihe ndị a esiji, a pụrụ ịsacha pigmenti ahụ, a pụkwara iji akpụkpọ ahụ mee ihe maka nyocha ọzọ.Mana agba anaghị agbanwe agbanwe, dị ka Coomassie na-acha anụnụ anụnụ, ink India, amido Black 10B, wdg, enweghị ike iji ihe nkiri nwere ntụpọ mee nyocha ọzọ.
Oge nzipu: Sep-09-2022
