Gwiritsani ntchitoguluu kulekana
zovuta ndi zovuta zofala:
1) Kukula pakati pa pepala losefera, guluu ndi nembanemba nthawi zambiri zimakhala pepala losefera> = membrane> = guluu.
2) Sipayenera kukhala thovu pakati pa fyuluta pepala, guluu ndi nembanemba, zomwe zingayambitse yochepa.
3) Chifukwa cha hydrophobicity ya nembanemba, nembanemba iyenera kunyowa kwathunthu mu methanol.Kuonjezera apo, filimuyo iyenera kukhala yonyowa nthawi zonse mu ntchito yotsatira (kupatulapo njira yowuma filimu).
4) Pepala losefera litha kugwiritsidwanso ntchito.Pali mapuloteni ambiri osinthidwa omwe amalowetsedwa mu pepala lapamwamba la fyuluta (membrane), kotero mapepala apamwamba ndi apansi sayenera kusakanikirana.Ngati sangathe kusiyanitsa, pepala la fyuluta la rabara likhoza kusinthidwa.
5) Nthawi yosinthira nthawi zambiri imakhala maola 1.5, (1ma-2ma / cm2, 10% gel osakaniza), ndipo nthawi yosinthira ndi yapano imatha kusinthidwa malinga ndi kulemera kwa maselo.
1) Pamene laminating filimu, guluu ndi mapepala masangweji, ntchito mu mbale yaikulu ndi kasinthasintha filimu buffer.Pambuyo pounjika, tumizani atatuwo ku chipangizo chozungulira cha filimu chofanana.Osawasunthanso, chifukwa kuthekera kwa thovu kwathetsedwa kwathunthu ndikugwira ntchito mu buffer, kotero palibe chifukwa choyendetsa thovu.
2) Pafupi nthawi yosinthira mafilimu: 20 V kwa semi dry rotation × 30min
(b) njira yonyowa
Sitigwiritsa ntchito njirayi, chifukwa chake sitiyiwonetsa pano.
C chizindikiritso cha zotsatira pambuyo metastasis
1. Kupaka utoto
Mutadetsedwa ndi Coomassie buluu wonyezimira komanso wonyezimira ndi Destain, onani ngati pali mapuloteni pagululi kuti awonetse momwe kusamutsa.
2. Kanema wa utoto
Pali mitundu iwiri yopangira utoto, yosinthika komanso yosasinthika.Zomwe zimasinthidwa zimaphatikizapo ponceau-s red, fastgreen FC, CPTs, etc. mutapaka utoto ndi utoto uwu, pigment ikhoza kutsukidwa, ndipo nembanemba ingagwiritsidwe ntchito pofufuzanso.Koma utoto wosasinthika, monga Coomassie brilliant blue, India inki, amido Black 10B, etc., ndi filimu yodetsedwa sangathe kugwiritsidwa ntchito pakuwunikanso.
Nthawi yotumiza: Sep-09-2022
