KUSUKELA ngo-1998

Umhlinzeki wesevisi we-one-stop wemishini yezokwelapha yokuhlinzwa evamile
isibhengezo_sekhanda

Ungayisebenzisa kanjani i-glue yokuhlukanisa?– Isiqephu sesi-2

Ungayisebenzisa kanjani i-glue yokuhlukanisa?– Isiqephu sesi-2

Imikhiqizo Ehlobene

Sebenzisaiglue yokuhlukanisa

izinkinga nezinkinga ezivamile:

1) Ubukhulu phakathi kwephepha lokuhlunga, iglue kanye nolwelwesi ngokuvamile kuyiphepha lokuhlunga > = ulwelwesi > = iglu.

2) Akumele kube namabhamuza phakathi kwephepha lokuhlunga, iglue kanye nolwelwesi, okuzodala ukujikeleza okufushane.

3) Ngenxa ye-hydrophobicity ye-membrane, i-membrane kufanele iqale imanziswe ngokuphelele ku-methanol.Ngaphezu kwalokho, ifilimu kufanele igcinwe imanzi ngaso sonke isikhathi ekusebenzeni okulandelayo (ngaphandle kwendlela yefilimu eyomile).

4) Iphepha lokuhlunga lingasetshenziswa kabusha.Kunamaprotheni amaningi adlulisiwe afakwe ephepheni lesihlungi eliphezulu (i-membrane), ngakho-ke amaphepha okuhlunga angaphezulu naphansi akumele ahlanganiswe.Uma zingakwazi ukuhlukaniswa, iphepha lokuhlunga irabha lingashintshwa.

I-5) Isikhathi sokudlulisa ngokuvamile amahora angu-1.5, (1ma-2ma / cm2, 10% ijeli), futhi isikhathi sokudlulisa kanye namanje kungashintshwa ngokuvumelana nesisindo samangqamuzana.

I-1) Uma ifilimu ye-laminating, i-glue kanye namasemishi wephepha, sebenzisa epuleti elikhulu elinesivikelo sefilimu esijikelezayo.Ngemva kokunqwabelanisa, dlulisela okuthathu kudivayisi yefilimu ezungezisayo ngokuhambisana.Ungabe usawanyakazisa, ngoba amathuba okuba amabhamuza asuswe ngokuphelele ngokusebenza ku-buffer, ngakho-ke asikho isidingo sokushayela amabhamuza.

I-2) Mayelana nesikhathi sokudlulisa ifilimu: I-20 V yokujikeleza kwe-semi dry × 30min

(b) indlela emanzi

Ngokuyisisekelo asisebenzisi le ndlela, ngakho-ke ngeke siyethule lapha.

C ukuhlonza umphumela ngemva kwe-metastasis

1. Ukudaya ngeglue

Ngemuva kokungcoliswa nge-Coomassie eluhlaza okwesibhakabhaka nokucwebezela kwe-Destain, bona ukuthi ingabe asekhona yini amaprotheni kuglue ukuze abonise umphumela wokudlulisa.

2. Ifilimu yokudaya

Kunezinhlobo ezimbili zezixazululo zikadayi, ezingahlehliswa futhi ezingahlehliseki.Okuhlehliswayo kuhlanganisa i-ponceau-s ebomvu, i-fastgreen FC, ama-CPT, njll. ngemva kokudaya ngalawa madayi, i-pigment ingakhukhulwa, futhi ulwelwesi lungasetshenziswa ukuze kuhlaziywe okwengeziwe.Kodwa odayi abangabuyiseleki emuva, abafana no-Coomassie brilliant blue, inki yase-India, i-amido Black 10B, njll., kanye nefilimu engcolile abanakusetshenziselwa ukuhlaziya okwengeziwe.

 

 

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Imikhiqizo Ehlobene
Isikhathi sokuthumela: Sep-09-2022