Sebenzisaiglue yokuhlukanisa
izinkinga nezinkinga ezivamile:
1) Ubukhulu phakathi kwephepha lokuhlunga, iglue kanye nolwelwesi ngokuvamile kuyiphepha lokuhlunga > = ulwelwesi > = iglu.
2) Akumele kube namabhamuza phakathi kwephepha lokuhlunga, iglue kanye nolwelwesi, okuzodala ukujikeleza okufushane.
3) Ngenxa ye-hydrophobicity ye-membrane, i-membrane kufanele iqale imanziswe ngokuphelele ku-methanol.Ngaphezu kwalokho, ifilimu kufanele igcinwe imanzi ngaso sonke isikhathi ekusebenzeni okulandelayo (ngaphandle kwendlela yefilimu eyomile).
4) Iphepha lokuhlunga lingasetshenziswa kabusha.Kunamaprotheni amaningi adlulisiwe afakwe ephepheni lesihlungi eliphezulu (i-membrane), ngakho-ke amaphepha okuhlunga angaphezulu naphansi akumele ahlanganiswe.Uma zingakwazi ukuhlukaniswa, iphepha lokuhlunga irabha lingashintshwa.
I-5) Isikhathi sokudlulisa ngokuvamile amahora angu-1.5, (1ma-2ma / cm2, 10% ijeli), futhi isikhathi sokudlulisa kanye namanje kungashintshwa ngokuvumelana nesisindo samangqamuzana.
I-1) Uma ifilimu ye-laminating, i-glue kanye namasemishi wephepha, sebenzisa epuleti elikhulu elinesivikelo sefilimu esijikelezayo.Ngemva kokunqwabelanisa, dlulisela okuthathu kudivayisi yefilimu ezungezisayo ngokuhambisana.Ungabe usawanyakazisa, ngoba amathuba okuba amabhamuza asuswe ngokuphelele ngokusebenza ku-buffer, ngakho-ke asikho isidingo sokushayela amabhamuza.
I-2) Mayelana nesikhathi sokudlulisa ifilimu: I-20 V yokujikeleza kwe-semi dry × 30min
(b) indlela emanzi
Ngokuyisisekelo asisebenzisi le ndlela, ngakho-ke ngeke siyethule lapha.
C ukuhlonza umphumela ngemva kwe-metastasis
1. Ukudaya ngeglue
Ngemuva kokungcoliswa nge-Coomassie eluhlaza okwesibhakabhaka nokucwebezela kwe-Destain, bona ukuthi ingabe asekhona yini amaprotheni kuglue ukuze abonise umphumela wokudlulisa.
2. Ifilimu yokudaya
Kunezinhlobo ezimbili zezixazululo zikadayi, ezingahlehliswa futhi ezingahlehliseki.Okuhlehliswayo kuhlanganisa i-ponceau-s ebomvu, i-fastgreen FC, ama-CPT, njll. ngemva kokudaya ngalawa madayi, i-pigment ingakhukhulwa, futhi ulwelwesi lungasetshenziswa ukuze kuhlaziywe okwengeziwe.Kodwa odayi abangabuyiseleki emuva, abafana no-Coomassie brilliant blue, inki yase-India, i-amido Black 10B, njll., kanye nefilimu engcolile abanakusetshenziselwa ukuhlaziya okwengeziwe.
Isikhathi sokuthumela: Sep-09-2022