UKUSUSELA ngo-1998

Umboneleli wenkonzo yotyando oluqhelekileyo lwezixhobo zonyango
intloko_ibhena

Indlela yokusebenzisa iglue yokwahlula?-Icandelo lesi-2

Indlela yokusebenzisa iglue yokwahlula?-Icandelo lesi-2

Iimveliso ezinxulumeneyo

Sebenzisaiglu yokwahlula

iingxaki kunye neengxaki eziqhelekileyo:

1) Ubungakanani phakathi kwephepha lokucoca, iglue kunye ne-membrane ngokuqhelekileyo iphepha lokucoca> = i-membrane> = iglue.

2) Akufuneki kubekho iibhubhu phakathi kwephepha lokucoca, iglue kunye ne-membrane, eya kubangela isiphaluka esifutshane.

3) Ngenxa ye-hydrophobicity ye-membrane, i-membrane kufuneka iqale ifakwe ngokupheleleyo kwi-methanol.Ukongezelela, ifilimu kufuneka igcinwe imanzi ngamaxesha onke ekusebenzeni okulandelayo (ngaphandle kwendlela yefilimu eyomileyo).

4) Iphepha lokucoca lingaphinda lisetyenziswe.Zininzi iiprotheyini ezigqithiselweyo ezifakwe kwiphepha eliphezulu lokucoca (imembrane), ngoko ke amaphepha okucoca aphezulu nasezantsi kufuneka angaxutywa.Ukuba azikwazi ukwahlula, iphepha lokucoca irabha linokutshintshwa.

I-5) Ixesha lokudlulisa ngokuqhelekileyo iiyure ze-1.5, (1ma-2ma / cm2, i-gel 10%), kwaye ixesha lokudlulisa kunye nangoku liyakwazi ukulungiswa ngokwesisindo se-molecular.

I-1) Xa ifilimu ye-laminating, i-glue kunye ne-sandwiches yephepha, isebenze kwiplate enkulu kunye ne-buffer yefilimu ejikelezayo.Emva kokupakisha, tshintshela ezi zintathu kwisixhobo sefilimu esijikelezayo ngokunxuseneyo.Ungaphindi uwashukumise, kuba ukubanakho kwamaqamu kupheliswe ngokupheleleyo ngokusebenza kwibuffer, ngoko akukho mfuneko yokuqhuba amaqamza.

I-2) Malunga nexesha lokudlulisa ifilimu: I-20 V ye-semi rotation eyomileyo × 30min

(b) indlela emanzi

Ngokwesiseko asisebenzisi le ndlela, ngoko ke asizukwazisa apha.

C ukuchongwa kwesiphumo emva kwe-metastasis

1. Ukudaya iglu

Emva kokungcoliswa nge-Coomassie eqaqambileyo eluhlaza okwesibhakabhaka kunye nokudityaniswa kwe-Destain, jonga ukuba kusekho iprotein kwiglu ebonisa isiphumo sokudluliselwa.

2. Ifilimu yedayi

Kukho iintlobo ezimbini zezisombululo zedayi, ezibuyiselwa umva kwaye ezingenakuguqulwa.Izinto ezibuyiselwayo ziquka i-ponceau-s ebomvu, i-fastgreen FC, i-CPTs, njl. emva kokudaya ngale dyes, i-pigment ingahlanjwa, kwaye i-membrane ingasetyenziselwa uhlalutyo olongezelelweyo.Kodwa iidayi ezingenakujikwa, ezinje ngeCoomassie brilliant blue, India inki, amido Black 10B, njl.

 

 

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Iimveliso ezinxulumeneyo
Ixesha lokuposa: Sep-09-2022