Yuav siv cov kua nplaum sib cais li cas?– part 2

Sivcais kua nplaum

Cov kev siv thiab cov teeb meem tshwm sim:

1) Qhov loj ntawm cov ntawv lim, kua nplaum thiab daim nyias nyias yog feem ntau lim ntawv > = membrane > = kua nplaum.

2) Yuav tsum tsis muaj npuas ntawm cov ntawv lim, kua nplaum thiab daim nyias nyias, uas yuav ua rau luv luv.

3) Vim yog hydrophobicity ntawm daim nyias nyias, daim nyias nyias yuav tsum tau ua ntej wetted tag nrho hauv methanol.Tsis tas li ntawd, cov yeeb yaj kiab yuav tsum tau ntub dej txhua lub sijhawm hauv kev ua haujlwm tom ntej (tshwj tsis yog rau txoj kev ua yeeb yaj kiab qhuav).

4) Cov ntawv lim tuaj yeem rov siv dua.Muaj ntau cov protein hloov pauv adsorbed nyob rau hauv cov ntawv lim sab saud (membrane), yog li cov ntawv lim sab saud thiab qis yuav tsum tsis txhob sib xyaw.Yog tias lawv tsis tuaj yeem paub qhov txawv, cov ntawv lim roj hmab tuaj yeem hloov tau.

5) Lub sijhawm hloov pauv feem ntau yog 1.5 teev, (1ma-2ma / cm2, 10% gel), thiab lub sijhawm hloov pauv thiab tam sim no tuaj yeem hloov kho raws li qhov hnyav molecular.

1) Thaum laminating zaj duab xis, kua nplaum thiab ntawv qhaub cij, ua haujlwm nyob rau hauv ib lub phaj loj nrog lub rotating zaj duab xis tsis.Tom qab stacking, hloov peb mus rau lub rotating zaj duab xis ntaus ntawv nyob rau hauv parallel.Tsis txhob txav lawv mus ntxiv, vim tias qhov ua tau ntawm cov npuas tau raug tshem tawm tag nrho los ntawm kev khiav hauj lwm hauv qhov tsis, yog li tsis tas yuav tsav npuas

2) Txog zaj duab xis hloov lub sij hawm: 20 V rau semi qhuav rotation × 30min