Shandisakupatsanura glue
matambudziko uye zvinowanzoitika:
1) Saizi iri pakati pesefa bepa, glue uye membrane kazhinji sefa bepa> = membrane> = glue.
2) Panofanira kunge pasina mabhubhu pakati pesefa bepa, glue uye membrane, izvo zvinokonzeresa kupfupika.
3) Nekuda kwe hydrophobicity ye membrane, membrane inofanira kutanga yanyoroveswa mu methanol.Mukuwedzera, iyo firimu inofanira kuchengetwa yakanyorova nguva dzose mukushanda kunotevera (kunze kweiyo yakaoma firimu nzira).
4) Pepa resefa rinogona kushandiswa zvakare.Kune akawanda mapuroteni akatamiswa akaiswa mubepa repamusoro resefa (membrane), saka mapepa ekumusoro neepasi esefa haafaniri kusanganiswa.Kana vasingakwanise kusiyaniswa, bepa rerubha sefa rinogona kutsiviwa.
5) Nguva yekufambisa inowanzoita maawa 1.5, (1ma-2ma / cm2, 10% gel), uye nguva yekufambisa uye ikozvino inogona kugadziriswa maererano nehuremu hwema molecular.
1) Paunenge uchiisa firimu, glue uye mapepa masangweji, shanda mundiro huru ine buffer yefirimu inotenderera.Mushure mekurongedza, endesa iwo matatu kune inotenderera firimu mudziyo wakafanana.Usazvifambisa zvakare, nekuti mukana wemabhubhu wakabviswa zvachose nekushanda mubuffer, saka hapana chikonzero chekutyaira mabhuru.
2) Nezvenguva yekufambisa firimu: 20 V yehafu yakaoma kutenderera × 30min
(b) nzira yemvura
Isu hatisi kushandisa nzira iyi, saka hatisi kuzoisuma pano.
C kuzivikanwa kwemhedzisiro mushure memetastasis
1. Glue dyeing
Mushure mekushatisa neCoomassie inopenya bhuruu uye decolorizing neDestain, ona kana pachine puroteni pane glue kuratidza maitiro ekutamisa.
2. Dhayi firimu
Kune marudzi maviri emhinduro dzedhayi, inodzoreredzwa uye isingadzokerike.Zvigadziriswa zvinosanganisira ponceau-s tsvuku, fastgreen FC, CPTs, nezvimwewo mushure mekudhaya nedhayi idzi, pigment inogona kushambidzwa, uye membrane inogona kushandiswa pakuwedzera kuongororwa.Asi madhayi asingadzoreki, akadai seCoomassie brilliant blue, India inki, amido Black 10B, nezvimwewo, uye firimu rakasvibiswa harigone kushandiswa pakuwedzera kuongororwa.
Nguva yekutumira: Sep-09-2022
