Hanyoyin Bincika don Rigunan da za a iya zubarwa don Rarraba Magunguna
1. Wannan hanyar dubawa tana amfani da sirinji masu zubar da ciki don rarrabawa.
Shirye-shiryen maganin gwaji
a.Ɗauki masu rarrabawa guda 3 ba da gangan ba daga nau'in samfuran iri ɗaya (ƙarar samfurin za a ƙayyade bisa ga ƙimar binciken da ake buƙata da ƙayyadaddun mai rarrabawa), ƙara ruwa zuwa samfurin zuwa ƙimar ƙima sannan a fitar da shi daga ganga mai tururi.Zuba ruwan a cikin akwati na gilashi a 37 ℃ ± 1 ℃ na 8h (ko 1h) kuma sanyaya shi zuwa zafin jiki a matsayin ruwan hakar.
b.Ajiye wani yanki na ruwa na ƙarar guda ɗaya a cikin akwati gilashi azaman bayani mara ƙarfi.
1.1 Abun ƙarfe mai cirewa
Saka 25ml na maganin cirewa a cikin 25ml Nessler colorimetric tube, ɗauki wani 25ml Nessler colorimetric tube, ƙara 25ml na gubar daidaitaccen maganin, ƙara 5ml na maganin gwajin sodium hydroxide zuwa manyan bututun launi guda biyu na sama, ƙara digo 5 na maganin gwajin sodium sulfide bi da bi, da kuma girgiza.Kada ya zama zurfi fiye da farin bango.
1.2 pH
Ɗauki bayani a da bayani b da aka shirya a sama kuma auna ƙimar pH ɗin su tare da acidimeta.Bambanci tsakanin su biyu za a ɗauka azaman sakamakon gwajin, kuma bambancin ba zai wuce 1.0 ba.
1.3 Ragowar ethylene oxide
1.3.1 Shirye-shiryen Magani: duba Karin I
1.3.2 Shirye-shiryen maganin gwaji
Za a shirya maganin gwajin nan da nan bayan samfurin, in ba haka ba za a rufe samfurin a cikin akwati don ajiya.
Yanke samfurin guda guda tare da tsawon 5mm, auna 2.0g kuma sanya shi a cikin akwati, ƙara 10ml na 0.1mol/L hydrochloric acid, kuma sanya shi a cikin dakin zafin jiki na 1h.
1.3.3 Matakan gwaji
① Ɗauki tubes masu launi na Nessler 5 kuma daidai ƙara 2ml na 0.1mol/L hydrochloric acid bi da bi, sannan ƙara daidai 0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml ethylene glycol misali bayani.Ɗauki wani bututu mai launi na Nessler kuma ƙara daidai 2ml na 0.1mol/L hydrochloric acid azaman iko mara kyau.
② Ƙara 0.4ml na 0.5% lokaci-lokaci acid bayani a cikin kowane daga cikin bututu na sama bi da bi da kuma sanya su na 1h.Sa'an nan kuma sauke sodium thiosulfate bayani har sai launin rawaya ya ɓace kawai.Sa'an nan kuma ƙara 0.2ml na fuchsin sulfurous acid gwajin bayani bi da bi, tsarma shi zuwa 10ml da distilled ruwa, sanya shi a dakin zafin jiki na 1h, da kuma auna absorbance a 560nm zangon tare da blank bayani a matsayin tunani.Zana madaidaicin juzu'i mai ɗaukar sha.
③ Canja wurin daidai 2.0ml na maganin gwajin cikin bututun launi na Nessler, kuma kuyi aiki daidai da mataki ②, don bincika madaidaicin ƙarar gwajin daga ma'auni mai lanƙwasa tare da auna abin sha.Yi lissafin ragowar ethylene oxide bisa ga dabara mai zuwa:
WEO=1.775V1 · c1
Inda: WEO -- zumunta abun ciki na ethylene oxide a cikin naúrar samfurin, mg/kg;
V1 - daidaitaccen ƙarar gwajin gwajin da aka samo akan daidaitaccen lanƙwasa, ml;
C1 - maida hankali na ethylene glycol daidaitaccen bayani, g / L;
Ragowar adadin ethylene oxide ba zai wuce 10ug/g ba.
1.4 Sauƙaƙe oxides
1.4.1 Shirye-shiryen Magani: duba Karin I
1.4.2 Shiri na gwajin maganin
Ɗauki 20ml na maganin gwajin da aka samu sa'a ɗaya bayan shiri na maganin cirewa a, kuma ɗauki b a matsayin maganin kulawa mara kyau.
1.4.3 Hanyoyin gwaji
A samu maganin cirewa 10ml, sai a zuba a cikin flask 250ml na iodine volumetric, a zuba 1ml na sulfuric acid (20%), daidai 10ml na 0.002mol/L potassium permanganate, zafi da tafasa don 3min, sauri kwantar da hankali, ƙara 0.1 g na potassium iodide, toshe tam, kuma girgiza sosai.Nan da nan titrate da sodium thiosulfate daidaitaccen bayani na taro iri ɗaya zuwa rawaya mai haske, ƙara digo 5 na maganin sitaci, kuma ci gaba da titrate tare da daidaitaccen bayani na sodium thiosulfate zuwa mara launi.
Titrate bayanin kula mara kyau tare da wannan hanya.
1.4.4 Lissafin sakamako:
Abubuwan da ke rage abubuwa (sauki oxides) ana bayyana su ta adadin maganin potassium permanganate da aka cinye:
V=
Inda: V - ƙarar amfani da maganin potassium permanganate, ml;
Vs - ƙarar sodium thiosulfate maganin da aka cinye ta hanyar gwajin gwajin, ml;
V0 - ƙarar maganin sodium thiosulfate wanda aka cinye ta hanyar bayani mara kyau, ml;
Cs -- ainihin maida hankali na titrated sodium thiosulfate bayani, mol/L;
C0 -- taro na potassium permanganate bayani da aka ƙayyade a cikin ma'auni, mol/L.
Bambanci a cikin amfani da bayani na potassium permanganate tsakanin maganin jiko na mai rarrabawa da kuma bayanin kula da maras kyau na nau'i ɗaya na ƙarar guda ɗaya zai zama ≤ 0.5ml.
Lokacin aikawa: Satumba-26-2022
