Hannaanka Kormeerka Siringaha La Tuuri Karo ee Bixinta Dawooyinka
1. Habkan kormeerka waxa lagu dabaqi karaa saliingadaha la tuuri karo ee lagu bixinayo.
Diyaarinta xalka tijaabada
a.Ka soo qaado 3 qaybiye si aan kala sooc lahayn oo isku dufcaddii alaabta ah (mugga muunada waa in lagu go'aamiyaa iyadoo loo eegayo cabbirka dareeraha ee loo baahan yahay iyo qeexidda qaybiyaha), ku dar biyo muunadda awoodda magaca oo ka soo saar durbaanka uumiga.Ku daadi biyaha weel galaas ah 37 ℃± 1 ℃ 8saac (ama 1saac) oo ku qabooji heerkulka qolka sida dareeraha soo saarista.
b.Ku kaydi qayb biyo ah oo isku mid ah weel quraarad ah sida xal kontorool maran.
1.1 Waxyaabaha birta ah ee la soo saari karo
Ku rid 25ml oo ah xalka soo saarista 25ml Nessler tube colorimetric, qaado 25ml Nessler colorimetric tube kale,ku dar 25ml ee xalinta sunta rasaasta,ku dar 5ml of sodium hydroxide xal tijaabo ah labada tubo midab ee kor ku xusan,ku dar 5 dhibcood oo ah xalka tijaabada sodium sulfide siday u kala horreeyaan, iyo ruxi.Waa inaanu ka sii qoto dheerayn asalka cad.
1.2 pH
Qaado xal a iyo xal b oo kor lagu diyaariyey kuna cabbir pH qiyamkooda aashitada.Farqiga u dhexeeya labada waa in loo qaataa sida natiijada imtixaanka, farqiga u dhexeeyaana waa inuusan dhaafin 1.0.
1.3 Hadhaaga ethylene oxide
1.3.1 Diyaarinta xalka: eeg Lifaaqa I
1.3.2 Diyaarinta xalka tijaabada
Xalka tijaabada waa in isla markiiba la diyaariyaa sambabada kadib, haddii kale muunada waa in lagu xidhaa weel lagu kaydin karo.
Iska yaree muunadda cad cad oo dhererkeedu yahay 5mm, miisaan 2.0g oo ku rid weel, ku dar 10ml oo ah 0.1mol/L hydrochloric acid, oo ku rid heerkulka qolka 1 saac.
1.3.3 Tijaabooyinka tijaabada
Qaado 5 tuubooyinka midabka Nessler oo si sax ah ugu dar 2ml oo ah 0.1mol/L hydrochloric acid siday u kala horreeyaan, ka dibna si sax ah ugu dar 0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml ethylene glycol solution standard.Qaado tuubo kale oo Nessler colorimetric ah oo si sax ah ugu dar 2ml oo ah 0.1mol/L hydrochloric acid sida xakamaynta banaan.
② Ku dar 0.4ml oo ah 0.5% xal aashito xilliyeed ah mid kasta oo ka mid ah tubooyinka kore siday u kala horreeyaan oo dhig 1 saac.Kadibna ku rid xalalka sodium thiosulfate ilaa midabka jaalaha ah uu meesha ka baxayo.Dabadeed ku dar 0.2ml ee fuchsin sulfurous acid xal siday u kala horreeyaan, ku milo 10ml biyo la miiray, ku rid heerkulka qolka 1h, oo cabbir nuugista 560nm mawjadda dhererka mawjadda maran sida tixraac ahaan.Sawir qalooca mugga nuugista.
③ Si sax ah ugu wareeji 2.0ml ee xalka tijaabada ah tuubada midabaynta ee Nessler, oo u shaqee si waafaqsan tillaaboXisaabi hadhaaga ethylene oxide ee saxda ah sida waafaqsan qaacidada soo socota:
WEO=1.775V1 · c1
Halkee: WEO -- nuxurka qaraabada ah ee ethylene oxide ee alaabta unugga, mg/kg;
V1 - mugga u dhigma ee xalka tijaabada ee laga helay qalooca caadiga ah, ml;
C1 --fiirsashada xalalka caadiga ah ee ethylene glycol, g/L;
Qadarka hadhaaga ethylene oxide waa in aanu ka badnayn 10ug/g.
1.4 oksaydhyo fudud
1.4.1 Diyaarinta xalka: eeg Lifaaqa I
1.4.2 Diyaarinta xalka tijaabada
Qaado 20ml ee xalka tijaabada ah ee la helay hal saac kadib diyaarinta xalka soo saarista a, oo qaado b sida xalka xakamaynta maran.
1.4.3 Hababka tijaabada
Qaado 10ml oo xalin ah, ku dar galaas 250ml oo iodine volumetric ah, ku dar 1ml oo sulfuric acid ah (20%), si sax ah ugu dar 10ml oo ah 0.002mol/L potassium permanganate, kulayl oo karkari 3min, si degdeg ah u qabooji, ku dar 0.1 g oo ah potassium iodide, si adag u xidh, oo si fiican u rux.Isla markiiba titrate leh sodium thiosulfate xal heerka isku-fiirsashada ah huruud iftiin ah, ku dar 5 dhibcood ee istaarijka xal tusaha, oo sii wad titrate leh sodium thiosulfate xal caadiga ah mid aan midab lahayn.
Titrate xalka kontoroolka maran hab la mid ah.
1.4.4 Xisaabinta natiijada:
Waxa ku jira dhimista walxaha (oxides fudud) waxaa lagu muujiyaa qadarka xalka potassium permanganate ee la isticmaalo:
V=
Halkee: V - mugga xalalka potassium permanganate ee la isticmaalo, ml;
Vs -- mugga thiosulfate sodium ee uu isticmaalo xalka tijaabada, ml;
V0 - mugga thiosulfate sodium ee lagu isticmaalo xal faaruq ah, ml;
Cs -- uruurinta dhabta ah ee titrated sodium thiosulfate, mol/L;
C0 ---fiirsashada xalka potassium permanganate ee lagu qeexay heerka, mol/L.
Farqiga u dhexeeya isticmaalka xalalka potassium permanganate ee u dhexeeya faleebada dareeraha iyo xalinta kontoroolka bannaan ee isla mugga isku midka ah waa inay ahaataa ≤ 0.5ml.
Waqtiga post: Seb-26-2022
