Uburyo bwo Kugenzura Siringi Zikoreshwa mu Gutanga Ibiyobyabwenge
1. Ubu buryo bwo kugenzura burakoreshwa kuri siringi ikoreshwa kugirango itangwe.
Gutegura igisubizo cyibizamini
a.Fata disipanseri 3 utabishaka uhereye kumurongo umwe wibicuruzwa (ingano yicyitegererezo igomba kugenwa hakurikijwe ibipimo bisabwa byamazi yagenzuwe hamwe nibisobanuro byatanzwe), ongeramo amazi kurugero rwubushobozi bwizina hanyuma ubisohore mungoma.Kuramo amazi mu kirahuri kuri 37 ℃ ± 1 ℃ kuri 8h (cyangwa 1h) hanyuma ukonje kugeza ku cyumba cy'ubushyuhe nk'amazi yo gukuramo.
b.Bika igice cyamazi yubunini bumwe mubirahuri nkibisubizo byubusa.
1.1 Ibirimo gukuramo ibyuma
Shira 25ml yumuti wo gukuramo muri 25ml Nessler colorimetric tube, fata indi 25ml ya Nessler colorimetric tube, ongeramo 25ml yumuti usanzwe wongeyeho, ongeramo 5ml yumuti wogupima sodium hydroxide mumiyoboro ibiri yavuzwe haruguru, ongeramo ibitonyanga 5 byumuti wa sodium sulfide. kunyeganyega.Ntabwo izaba yimbitse kurenza inyuma yera.
1.2 pH
Fata igisubizo a nigisubizo b cyateguwe hejuru hanyuma upime agaciro ka pH hamwe na acide.Itandukaniro riri hagati yombi rifatwa nkibisubizo byikizamini, kandi itandukaniro ntirishobora kurenga 1.0.
1.3 Okiside ya Ethylene isigaye
1.3.1 Gutegura igisubizo: reba Umugereka I.
1.3.2 Gutegura igisubizo cyibizamini
Igisubizo cyikizamini kigomba gutegurwa ako kanya nyuma yicyitegererezo, bitabaye ibyo icyitegererezo kigomba gufungirwa mububiko.
Kata icyitegererezo mo ibice bifite uburebure bwa 5mm, upima 2.0g hanyuma ubishyire muri kontineri, ongeramo 10ml ya 0.1mol / L aside hydrochloric, hanyuma ubishyire mubushyuhe bwicyumba cya 1h.
1.3.3 Intambwe yikizamini
① Fata 5 Nessler colorimetric tubes hanyuma ongeramo neza 2ml ya 0.1mol / L ya hydrochloric aside, hanyuma wongereho neza 0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml ya Ethylene glycol yumuti usanzwe.Fata indi Nessler colorimetric tube hanyuma wongereho neza 2ml ya 0.1mol / L hydrochloric aside nkigenzura ryuzuye.
② Ongeramo 0.4ml ya 0.5% yumuti wa acide buri gihe muri buri muyoboro wavuzwe haruguru hanyuma ubishyire kuri 1h.Noneho fata sodium thiosulfate igisubizo kugeza ibara ry'umuhondo rishize.Noneho ongeramo 0.2ml ya fuchsin sulfurous aside yipimishije, ukayungurura kugeza kuri 10ml hamwe namazi yatoboye, ukayashyira mubushyuhe bwicyumba cya 1h, hanyuma ugapima iyinjizwa ryumurambararo wa 560nm hamwe nigisubizo cyubusa nkibisobanuro.Shushanya uburyo bwo gukuramo amajwi asanzwe.
Kohereza neza 2.0ml yumuti wikizamini muri Nessler ya colorimetric tube, hanyuma ukore ukurikije intambwe ②, kugirango ugenzure ingano ijyanye nikizamini uhereye kumurongo usanzwe hamwe no gupimwa kwapimwe.Kubara ibisigisigi bya Ethylene bisigaye ukurikije formula ikurikira:
WEO = 1.775V1 · c1
Aho: WEO - ugereranije nibintu bya okiside ya Ethylene mubicuruzwa, mg / kg;
V1 - ingano ijyanye nigisubizo cyibizamini kiboneka kumurongo usanzwe, ml;
C1 - kwibanda kuri Ethylene glycol igisubizo gisanzwe, g / L;
Umubare usigaye wa okiside ya Ethylene ntushobora kurenza 10ug / g.
1.4 Okiside yoroshye
1.4.1 Gutegura igisubizo: reba Umugereka I.
1.4.2 Gutegura igisubizo cyibizamini
Fata 20ml yumuti wikizamini wabonye isaha imwe nyuma yo gutegura igisubizo cyo gukuramo a, hanyuma ufate b nkigisubizo cyubusa.
1.4.3 Uburyo bwo gukora ibizamini
Fata 10ml yumuti wo gukuramo, ongeramo flask ya 250ml iyode yuzuye, ongeramo 1ml ya acide sulfurike ya acide (20%), ongeramo neza 10ml ya 0.002mol / L potassium permanganate yumuti, ubushyuhe hanyuma ubiteke kuri 3min, bikonje vuba, ongeramo 0.1 g ya potasiyumu iyode, ucomeka neza, kandi uzunguze neza.Ako kanya witondere hamwe na sodiumi thiosulfate igisubizo gisanzwe cyumubyigano umwe kumuhondo werurutse, ongeramo ibitonyanga 5 byumuti werekana ibimenyetso bya krahisi, hanyuma ukomeze gutanga titre hamwe na sodium thiosulfate igisubizo gisanzwe kitagira ibara.
Andika igisubizo cyubusa hamwe nuburyo bumwe.
1.4.4 Kubara ibisubizo:
Ibirimo kugabanya ibintu (okiside yoroshye) bigaragazwa nubunini bwa potasiyumu permanganate ikoreshwa:
V=
Aho: V - ingano ya potasiyumu ya permanganate yakoreshejwe, ml;
Vs - ingano yumuti wa sodium thiosulfate ikoreshwa nigisubizo cyikizamini, ml;
V0 - ingano yumuti wa sodiumi thiosulfate ikoreshwa nigisubizo cyambaye ubusa, ml;
Cs - kwibanda kwukuri kwa sodiumi thiosulfate yumuti, mol / L;
C0 - kwibanda kuri potasiyumu permanganate igisubizo cyerekanwe mubisanzwe, mol / L.
Itandukaniro ryo gukoresha umuti wa potasiyumu permanganate hagati yumuti winjiza wa dispanseri hamwe nigisubizo cyubusa cyicyiciro kimwe cyubunini bumwe kizaba ≤ 0.5ml.
Igihe cyo kohereza: Nzeri-26-2022
