Mekhoa ea Tlhahlobo ea Lisyringe tse Lahloang bakeng sa Phano ea Lithethefatsi
1. Mokhoa ona oa tlhahlobo o sebetsa ho li-syringe tse lahliloeng bakeng sa ho fana.
Tokisetso ea tharollo ea teko
a.Nka li-dispensers tse 3 ka mokhoa o sa reroang ho tsoa sehlopheng se le seng sa lihlahisoa (bolumo ba sampole bo tla khethoa ho latela tekanyo e hlokahalang ea tlhahlobo ea mokelikeli le tlhahiso ea li-dispenser), eketsa metsi ho sampole ho matla a lebitso ebe o a ntša moqomong oa mouoane.Hlakola metsi ka har'a sets'oants'o sa khalase ka 37 ℃± 1 ℃ bakeng sa 8h (kapa 1h) 'me u a pholisoe ka mocheso oa kamore e le mokelikeli o ntšoang.
b.Beha karolo ea metsi a boholo bo lekanang ka sejaneng sa khalase e le tharollo e se nang letho ea taolo.
1.1 Likahare tsa tšepe tse ntšitsoeng
Kenya 25ml ea tharollo ea ho ntšoa ka har'a 25ml Nessler colorimetric tube, nka e 'ngoe ea 25ml Nessler colorimetric tube, eketsa 25ml ea tharollo e tloaelehileng ea lead, eketsa 5ml ea tharollo ea tlhahlobo ea sodium hydroxide ho li-tubes tse peli tse kaholimo, eketsa marotholi a 5 a tharollo ea tlhahlobo ea sodium sulfide ka ho latellana, le sisinya.E ke ke ea teba ho feta bokantle bo bosoeu.
1.2 pH
Nka tharollo a le tharollo b e lokisitsoeng ka holimo 'me u lekanye boleng ba bona ba pH ka acidimeter.Phapang pakeng tsa tse peli e tla nkoa e le sephetho sa teko, 'me phapang e ke ke ea feta 1.0.
1.3 Ethylene oxide e setseng
1.3.1 Tokiso ea tharollo: sheba Sehlomathiso sa I
1.3.2 Tokisetso ea tharollo ea teko
Tharollo ea teko e tla lokisoa hang ka mor'a ho etsa sampole, ho seng joalo sampole e tla koalloa ka sejaneng bakeng sa polokelo.
Khaola mohlala ka likotoana ka bolelele ba 5mm, boima ba 2.0g 'me ue behe ka har'a setshelo, eketsa 10ml ea 0.1mol / L hydrochloric acid,' me ue behe mocheso oa kamore bakeng sa 1h.
1.3.3 Mehato ea liteko
① Nka li-tubes tse 5 tsa Nessler colorimetric 'me u kenye ka nepo 2ml ea 0.1mol/L hydrochloric acid ka ho latellana, ebe u eketsa ka nepo 0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml ethylene glycol tharollo e tloaelehileng.Nka karolo e 'ngoe ea Nessler colorimetric 'me u kenye ka nepo 2ml ea 0.1mol/L hydrochloric acid e le taolo e se nang letho.
② Eketsa 0.4ml ea 0.5% ea tharollo ea acidic periodic ho e 'ngoe le e' ngoe ea li-tubes tse ka holimo ka ho latellana ebe u li beha bakeng sa 1h.Ebe o theola tharollo ea sodium thiosulfate ho fihlela 'mala o mosehla o nyamela feela.Ebe u eketsa 0.2ml ea tharollo ea fuchsin sulfurous acid test ka ho latellana, e hlapolle ho 10ml ka metsi a hloekisitsoeng, e behe mocheso oa kamore bakeng sa 1h, 'me u lekanye monko ho 560nm wavelength ka tharollo e se nang letho.Draw volume volume standard curve.
③ Fetisetsa ka nepo 2.0ml ea tharollo ea teko ka har'a tube ea colorimetric ea Nessler, 'me u sebetse ho latela mohato ②, e le ho lekola bophahamo bo lekanang ba tlhahlobo ho tloha mothating o tloaelehileng le monyetla o lekantsoeng.Bala masala a ethylene oxide a felletseng ho latela foromo e latelang:
WEO=1.775V1 · c1
Moo: WEO -- litaba tse amanang le ethylene oxide sehlahisoa sa unit, mg/kg;
V1 - molumo o lekanang oa tharollo ea teko e fumanoang moqomong o tloaelehileng, ml;
C1 -- mahloriso a tharollo e tloaelehileng ea ethylene glycol, g/L;
Palo e setseng ea ethylene oxide ha ea lokela ho feta 10ug/g.
1.4 Li-oxide tse bonolo
1.4.1 Tokiso ea tharollo: sheba Sehlomathiso sa I
1.4.2 Tokisetso ea tharollo ea teko
Nka 20ml ea tharollo ea teko e fumanoeng hora e le 'ngoe ka mor'a ho lokisoa ha tharollo ea ho ntša a, 'me u nke b e le tharollo e se nang letho ea taolo.
1.4.3 Mekhoa ea liteko
Nka 10ml ea tharollo ea mokelikeli, e kenye ka botlolong ea 250ml ea iodine volumetric, eketsa 1ml ea dilute sulfuric acid (20%), ka nepo eketsa 10ml ea 0.002mol / L potassium permanganate solution, futhumatsa 'me u belise metsotso e 3, phola ka potlako, eketsa 0.1 g ea iodide ea potasiamo, koala ka thata, 'me u sisinyehe hantle.Hang-hang titrate ka sodium thiosulfate standard solution ea mahloriso a ts'oanang ho mosehla o khanyang, eketsa marotholi a 5 a tharollo ea starch indicator, 'me u tsoele pele ho tsitsisa ka tharollo e tloaelehileng ea sodium thiosulfate ho se nang mebala.
Titrate tharollo e se nang letho ea taolo ka mokhoa o ts'oanang.
1.4.4 Palo ea Sephetho:
Likahare tsa lintho tse fokotsang (li-oxide tse bonolo) li hlahisoa ke tekanyo ea tharollo ea potassium permanganate e jeoang:
V=
Moo: V -- bophahamo ba metsi a tharollo ea potassium permanganate, ml;
Vs - boholo ba tharollo ea sodium thiosulfate e jeoang ke tharollo ea teko, ml;
V0 - tekanyo ea tharollo ea sodium thiosulfate e jeoang ke tharollo e se nang letho, ml;
Cs - khatello ea 'nete ea tharollo ea sodium thiosulfate e titrated, mol/L;
C0 -- mahloriso a tharollo ea potassium permanganate e boletsoeng ka mokhoa o tloaelehileng, mol/L.
Phapang ea tšebeliso ea tharollo ea potassium permanganate pakeng tsa tharollo ea infusion ea dispenser le tharollo e se nang letho ea batch e tšoanang ea molumo o tšoanang e tla ba ≤ 0.5ml.
Nako ea poso: Sep-26-2022
