Izinqubo Zokuhlola Izirinji Ezilahlwayo Zokukhipha Izidakamizwa
1. Le nqubo yokuhlola iyasebenza kumasirinji alahlwayo ukuze akhishwe.
Ukulungiswa kwesixazululo sokuhlola
a.Thatha ama-dispensers ama-3 ngokungahleliwe eqoqweni elifanayo lemikhiqizo (ivolumu yesampula izonqunywa ngokuya ngevolumu yoketshezi lokuhlola elidingekayo kanye nokucaciswa kwe-dispenser), engeza amanzi kusampula kumthamo omncane bese uwakhipha esigubhu sesitimu.Khipha amanzi esitsheni sengilazi ku-37 ℃± 1 ℃ amahora angu-8 (noma u-1h) bese uwapholisa ekamelweni lokushisa njengoketshezi olukhiphayo.
b.Gcina ingxenye yamanzi yevolumu efanayo esitsheni sengilazi njengesixazululo sokulawula esingenalutho.
1.1 Okuqukethwe kwensimbi ekhiphekayo
Faka u-25ml wesixazululo sokukhipha ku-25ml Nessler colorimetric tube, thatha elinye ishubhu le-Nessler colorimetric elingu-25ml, engeza u-25ml wesisombululo esijwayelekile somthofu, engeza u-5ml wesixazululo sokuhlola i-sodium hydroxide kulawa mashubhu ombala amabili angenhla, engeza amaconsi ama-5 esixazululo sokuhlola i-sodium sulfide ngokulandelana, futhi qhaqhazela.Akumele ijule kunengemuva elimhlophe.
1.2 pH
Thatha isixazululo u-a nesixazululo b esilungiselelwe ngenhla bese ukala amanani azo we-pH nge-acidimeter.Umehluko phakathi kwakho kokubili uzothathwa njengomphumela wokuhlolwa, futhi umehluko ngeke udlule u-1.0.
1.3 I-ethylene oxide esele
1.3.1 Ukulungiswa kwesixazululo: bheka iSithasiselo I
1.3.2 Ukulungiswa kwesixazululo sokuhlola
Isixazululo sokuhlola sizolungiswa ngokushesha ngemva kokuthatha isampula, ngaphandle kwalokho isampula izovalwa esitsheni ukuze igcinwe.
Sika isampula libe yizicucu ngobude obungu-5mm, ukale u-2.0g bese uyibeka esitsheni, engeza u-10ml ka-0.1mol/L we-hydrochloric acid, futhi uyibeke ekamelweni lokushisa ihora elingu-1.
1.3.3 Izinyathelo zokuhlola
① Thatha amashubhu embala we-Nessler angu-5 bese wengeza ngokunembile u-2ml ka-0.1mol/L we-hydrochloric acid ngokulandelana, bese wengeza ngokunembile u-0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml isixazululo esijwayelekile se-ethylene glycol.Thatha elinye ishubhu le-colorimetric le-Nessler bese wengeza ngokunembile u-2ml ka-0.1mol/L hydrochloric acid njengesilawuli esingenalutho.
② Faka u-0.4ml ka-0.5% wesisombululo se-acidic periodic kushubhu ngalinye kwangenhla ngokulandelana bese ulibeka ihora elingu-1.Bese uphonsa isisombululo se-sodium thiosulfate kuze kube yilapho umbala ophuzi uvele unyamalale.Bese wengeza u-0.2ml wesixazululo sokuhlola i-fuchsin sulfurous acid ngokulandelana, usihlambulule sibe ngu-10ml ngamanzi acwecwe, usibeke ekamelweni lokushisa ihora elingu-1, bese ukala ukumunca ku-560nm wavelength ngesixazululo esingenalutho njengereferensi.Dweba ijika elijwayelekile levolumu yokumunca.
③ Dlulisa ngokunembile u-2.0ml wesixazululo sokuhlola kushubhu ye-colorimetric ye-Nessler, futhi usebenze ngokuya ngesinyathelo ②, ukuze uhlole ivolumu ehambisanayo yokuhlola ukusuka kwijika elijwayelekile nokumunca okulinganiselwe.Bala izinsalela ze-ethylene oxide ngokuphelele ngokwefomula elandelayo:
WEO=1.775V1 · c1
Lapho: I-WEO -- okuqukethwe okuhlobene kwe-ethylene oxide emkhiqizweni weyunithi, mg/kg;
I-V1 - ivolumu ehambisanayo yesisombululo sokuhlola esitholakala kwijika elijwayelekile, ml;
C1 -- ukugxila kwesisombululo esijwayelekile se-ethylene glycol, g/L;
Inani elisele le-ethylene oxide akufanele libe ngaphezu kuka-10ug/g.
1.4 Ama-oxide alula
1.4.1 Ukulungiswa kwesixazululo: bheka iSithasiselo I
1.4.2 Ukulungiswa kwesixazululo sokuhlola
Thatha u-20ml wekhambi lokuhlola elitholwe ihora elilodwa ngemva kokulungiswa kwesixazululo sokukhipha u-a, bese uthatha u-b njengekhambi lokulawula elingenalutho.
1.4.3 Izinqubo zokuhlola
Thatha u-10ml wekhambi lokukhipha, ulifake ku-250ml ye-iodine volumetric flask, wengeze u-1ml we-dilute sulfuric acid (20%), engeza ngokunembile u-10ml we-0.002mol/L yesisombululo se-potassium permanganate, ukushisa bese ubilisa imizuzu emithathu, pholisa ngokushesha, engeza u-0.1 g ye-iodide ye-potassium, vala ngokuqinile, futhi unyakazise kahle.Ngokushesha gcoba ngesisombululo esijwayelekile se-sodium thiosulfate sokugxiliswa okufanayo kokuphuzi ngokukhanyayo, engeza amaconsi angu-5 esisombululo senkomba yesitashi, bese uqhubeka nokunyakazisa ngesisombululo esivamile se-sodium thiosulfate kokungenambala.
Titrate isixazululo sokulawula esingenalutho ngendlela efanayo.
1.4.4 Ukubalwa kwemiphumela:
Okuqukethwe kwezinto ezinciphisayo (ama-oxide alula) kuvezwa inani lesixazululo se-potassium permanganate esidliwe:
V=
Kuphi: V - umthamo wesisombululo se-potassium permanganate, ml;
Vs -- umthamo wesisombululo se-sodium thiosulfate esisetshenziswe isisombululo sokuhlola, ml;
I-V0 - ivolumu yesisombululo se-sodium thiosulfate esetshenziswa isixazululo esingenalutho, ml;
Cs -- ukuhlushwa kwangempela kwesisombululo se-sodium thiosulfate, i-mol/L;
C0 -- ukugxila kwesisombululo se-potassium permanganate esicaciswe kuzinga, i-mol/L.
Umehluko ekusetshenzisweni kwesisombululo se-potassium permanganate phakathi kwesisombululo sokumnika se-dispenser kanye nesisombululo sokulawula esingenalutho seqoqo elifanayo levolumu efanayo kuyoba ≤ 0.5ml.
Isikhathi sokuthumela: Sep-26-2022
