Iinkqubo zokuHlola iisirinji eziLahlwayo zoKukhupha iChiza
1. Le nkqubo yokuhlola isetyenziswa kwiisirinji ezilahlwayo zokukhupha.
Ukulungiswa kwesisombululo sovavanyo
a.Thatha i-3 dispensers ngokungaqhelekanga kwi-batch efanayo yeemveliso (umthamo wesampulu uya kumiselwa ngokuhambelana nomthamo ofunekayo wokuhlola ulwelo kunye nenkcazo ye-dispenser), yongeza amanzi kwisampulu kumthamo we-nominal kwaye uyikhuphe kwi-drum yomphunga.Khupha amanzi kwisingxobo seglasi nge-37 ℃± 1 ℃ i-8h (okanye i-1h) kwaye uwapholise kwiqondo lobushushu begumbi njengolwelo lokutsalwa.
b.Gcina inxalenye yamanzi yomthamo ofanayo kwisitya seglasi njengesisombululo sokulawula esingenanto.
1.1 Umxholo wesinyithi okhutshwayo
Faka i-25ml yesisombululo sokutsalwa kwi-25ml ye-Nessler colorimetric tube, thatha enye i-25ml ye-Nessler colorimetric ityhubhu, yongeza i-25ml yesisombululo esisemgangathweni, yongeza i-5ml yesisombululo sovavanyo lwe-sodium hydroxide kwezi zityhubhu zombala zimbini zingasentla, yongeza amathontsi ama-5 esisombululo sovavanyo lwe-sodium sulfide ngokulandelelana, kwaye hlukuhlele phezulu.Ayiyi kuba nzulu kunomva omhlophe.
1.2 pH
Thatha isinyibiliko u-a kunye nesinyibiliko b esilungiswe ngasentla kwaye ulinganise amaxabiso azo pH nge-acidimeter.Umahluko phakathi kwezi zibini uya kuthathwa njengesiphumo sovavanyo, kwaye umahluko awuyi kudlula i-1.0.
1.3 Intsalela ye-ethylene oxide
1.3.1 Ukulungiswa kwesisombululo: jonga kwiSihlomelo I
1.3.2 Ukulungiswa kwesisombululo sovavanyo
Isisombululo sovavanyo siya kulungiswa ngokukhawuleza emva kwesampuli, ngaphandle koko isampuli iya kutywinwa kwisitya sokugcina.
Sika isampuli ibe ngamaqhekeza kunye nobude be-5mm, ulinganise i-2.0g kwaye uyibeke kwisitya, yongeza i-10ml ye-0.1mol / L i-hydrochloric acid, kwaye uyibeke kwindawo yokushisa kwe-1h.
1.3.3 Amanyathelo ovavanyo
① Thatha i-5 Nessler colorimetric tubes kwaye udibanise ngokuchanekileyo i-2ml ye-0.1mol / L i-hydrochloric acid ngokulandelanayo, uze udibanise ngokuchanekileyo i-0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml isisombululo esiqhelekileyo se-ethylene glycol.Thatha enye i-Nessler colorimetric tube kwaye udibanise ngokuchanekileyo i-2ml ye-0.1mol / L i-hydrochloric acid njengolawulo olungenanto.
② Yongeza i-0.4ml ye-0.5% isisombululo se-asidi ye-periodic kwi-tube nganye kwezi zingentla ngokulandelanayo kwaye uzibeke kwi-1h.Emva koko lahla isisombululo se-sodium thiosulfate de umbala ophuzi uvele uphele.Emva koko yongeza i-0.2ml yesisombululo sovavanyo lwe-fuchsin sulfurous acid ngokulandelanayo, yihlambulule kwi-10ml ngamanzi adibeneyo, uyibeke kwindawo yokushisa ye-1h, kwaye ulinganise ukuxhamla kwi-560nm wavelength kunye nesisombululo esingenanto njengereferensi.Zoba igophe elisezantsi lomthamo wevolumu.
③ Ukudlulisela ngokuchanekileyo i-2.0ml yesisombululo sovavanyo kwi-tube ye-colorimetric ka-Nessler, kwaye usebenze ngokwenyathelo ②, ukuze ukhangele umthamo ohambelanayo wovavanyo ukusuka kwi-curve esemgangathweni kunye ne-absorbence elinganisiweyo.Bala intsalela ye-ethylene oxide ngokupheleleyo ngokwefomula ilandelayo:
WEO=1.775V1 · c1
Apho: I-WEO -- isiqulatho esihambelanayo se-ethylene oxide kwimveliso yeyunithi, mg/kg;
I-V1 - umthamo ohambelanayo wesisombululo sovavanyo olufunyenwe kwi-curve standard, ml;
I-C1 -- i-concentration ye-ethylene glycol isisombululo esisemgangathweni, i-g / L;
Umlinganiselo oshiyekileyo we-ethylene oxide awuyi kuba ngaphezu kwe-10ug/g.
1.4 Ii-oksidi ezilula
1.4.1 Ukulungiswa kwesisombululo: jonga kwiSihlomelo I
1.4.2 Ukulungiswa kwesisombululo sovavanyo
Thatha i-20ml yesisombululo sovavanyo efunyenwe iyure enye emva kokulungiswa kwesisombululo sokutsalwa, kwaye uthathe i-b njengesisombululo solawulo olungenanto.
1.4.3 Iinkqubo zovavanyo
Thatha i-10ml yesisombululo sokukhupha, uyifake kwi-250ml ye-iodine volumetric flask, yongeza i-1ml ye-dilute sulfuric acid (20%), yongeza ngokuchanekileyo i-10ml ye-0.002mol / L yesisombululo se-potassium permanganate, ubushushu kwaye ubilise i-3min, upholise ngokukhawuleza, udibanise i-0.1 g ye-potassium iodide, iplagi ngokuqinileyo, kwaye uvuthulule kakuhle.Kwangoko itrate ngesisombululo esisemgangathweni se-sodium thiosulfate yoxinaniso olufanayo ukuya kumthubi okhanyayo, yongeza amathontsi ama-5 esisombululo sesalathisi sesitatshi, kwaye uqhubeke uthambisa ngesisombululo esiqhelekileyo se-sodium thiosulfate kwi-colorless.
Tshatisa isisombululo solawulo esingenanto ngendlela efanayo.
1.4.4 Ubalo lweziphumo:
Umxholo wezinto zokunciphisa (ii-oxide ezilula) zibonakaliswa ngumlinganiselo wesisombululo se-potassium permanganate esetyenzisiweyo:
V=
Apho: V -- umthamo wesisombululo esisetyenzisiweyo se-potassium permanganate, ml;
Vs - umthamo wesisombululo se-sodium thiosulfate esisetyenziswe sisisombululo sovavanyo, ml;
I-V0 - umthamo wesisombululo se-sodium thiosulfate esisetyenziswe ngesisombululo esingenanto, ml;
I-Cs -- i-concentration yangempela ye-titrated sodium thiosulfate isisombululo, i-mol / L;
C0 -- ugxininiso lwesisombululo sepotassium permanganate esixelwe kumgangatho, mol/L.
Umahluko ekusetyenzisweni kwesisombululo se-potassium permanganate phakathi kwesisombululo se-infusion ye-dispenser kunye nesisombululo sokulawula esingenanto se-batch efanayo yomthamo ofanayo uya kuba ≤ 0.5ml.
Ixesha lokuposa: Sep-26-2022
