Mafungiro ekutanga ane hukama nekusimudzira coagulation

Anticoagulant musimboti uye kushandiswa kweakajairika anticoagulants

1. Heparin ndiyo inosarudzwa anticoagulant yekuona makemikari eropa.Heparin is mucopolysaccharide ine sulfate boka, uye chiyero che molecular uremu hwechikamu chakapararira ndeye 15000. Iro anticoagulation nheyo inonyanya kusanganiswa ne antithrombin III kukonzera kuchinja mukugadziriswa kwe antithrombin III uye kukurumidza kuumbwa kwethrombin thrombin complex kuti ibudise anticoa. .Mukuwedzera, heparin inogona kudzivisa thrombin nerubatsiro rweplasma cofactor (heparin cofactor II).Kazhinji heparin anticoagulants sodium, potassium, lithium uye ammonium salts yeheparin, pakati peiyo lithium heparin ndiyo yakanakisisa, asi mutengo wayo unodhura.Sodium uye potassium munyu ichawedzera zvirimo sodium uye potassium muropa, uye ammonium munyu achawedzera zviri urea nitrogen.Muyero weheparin we anticoagulation kazhinji 10. 0 ～ 12.5 IU/ml ropa.Heparin ine zvishoma kupindira nezvikamu zveropa, haikanganisi huwandu hwemasero matsvuku eropa, uye haikonzeri hemolysis.Inokodzera kuyerwa kwesero permeability bvunzo, gasi reropa, plasma permeability, hematocrit uye general biochemical kutsunga.Nekudaro, heparin ine antithrombin mhedzisiro uye haina kukodzera kuongororwa kweropa coagulation.Mukuwedzera, heparin yakawandisa inogona kukonzera leukocyte aggregation uye thrombocytopenia, saka haina kukodzera kusarudzwa kweleukocyte uye kuverengwa kweplatelet, kana kuongororwa kwehemostasis Mukuwedzera, heparin anticoagulation haigoni kushandiswa kugadzira ropa smears, nokuti rima rebhuruu rinoonekwa mushure meWright staining. , iyo inokanganisa kuderedzwa kwekugadzirwa kwema microscopic.Heparin anticoagulation inofanira kushandiswa kwenguva pfupi, kana zvisina kudaro ropa rinogona kubatana mushure mekuiswa kwenguva yakareba.

2. EDTA munyu.EDTA inogona kusanganisa neCa2+ muropa kugadzira chelate.Iyo coagulation process yakavharwa uye ropa harigone kugadzika EDTA munyu unosanganisira potassium, sodium uye lithium munyu.Sangano reInternational Hematology Standardization Committee rinokurudzira kushandiswa kweEDTA-K2, iyo ine yakanyanya kunyungudika uye inokurumidza anticoagulation kumhanya.EDTA munyu unowanzo gadzirirwa mumushonga une aqueous ine chikamu chikuru che15%.Wedzera 1.2mgEDTA per ml yeropa, kureva, wedzera 0.04ml ye15% EDTA mhinduro pa5ml yeropa.EDTA munyu inogona kuomeswa pa100 ℃, uye mhedzisiro yayo yeanticoagulation inoramba isina kuchinjika EDTA munyu haikanganise kuverengeka kwemasero machena eropa uye saizi, ine mhedzisiro shoma pane morphology yemasero matsvuku eropa, inhibits platelet aggregation, uye inokodzera general hematological. kuonekwa.Kana kusanganiswa kweanticoagulant kwakanyanyisa, iyo osmotic pressure ichakwira, iyo ichaita kuti sero shrinkage Iyo pH ye EDTA mhinduro ine hukama hukuru nemunyu, uye pasi pH inogona kukonzera kuwedzera kwesero.EDTA-K2 inogona kuwedzera zvishoma huwandu hwemasero matsvuku eropa, uye avhareji yeplatelet vhoriyamu munguva pfupi mushure mekuunganidzwa kweropa haina kugadzikana uye inoita kunge yakagadzikana mushure mehafu yeawa.EDTA-K2 yakaderera Ca2+, Mg2+, creatine kinase uye alkaline phosphatase.Iyo yakakwana yekusangana kweEDTA-K2 yaive 1. 5mg/ml ropa.Kana paine ropa shoma, neutrophils ichazvimba, lobulate uye kunyangarika, maplatelet achazvimba uye kuparara, achigadzira zvimedu zveakajairwa maplatelet, izvo zvinozotungamira mukukanganisa muongororo mhedzisiro EDTA munyu inogona kutadzisa kana kupindira ne polymerization yefibrin monomers panguva yekuumbwa. yefibrin clots, iyo isingakodzeri kuonekwa kweropa coagulation uye platelet function, kana kuti kugadzirisa calcium, potassium, sodium uye nitrogenous zvinhu.Mukuwedzera, EDTA inogona kukanganisa basa remamwe ma enzyme uye inhibit lupus erythematosus factor, saka haina kukodzera kugadzira histochemical staining uye kuongorora ropa smear ye lupus erythematosus masero.

3. Citrate inonyanya sodium citrate.Yayo anticoagulation nheyo ndeyokuti inogona kusanganiswa neCa2 + muropa kuti iite chelate, kuitira kuti Ca2 + inorasikirwa nebasa rayo rekugadzirisa uye iyo coagulation process yakavharwa, nokudaro kudzivirira ropa coagulation.Sodium citrate ine marudzi maviri emakristasi, Na3C6H5O7 · 2H2O uye 2Na3C6H5O7 · 11H2O, kazhinji 3.8% kana 3 neyokutanga.2% aqueous solution, yakasanganiswa neropa muhuwandu hwe1: 9.Yakawanda coagulation bvunzo inogona kuva anticoagulated ne sodium citrate, iyo inobatsira kugadzikana kwechinhu V uye factor VIII, uye ine zvishoma zvinokanganisa paavhareji yeplatelet vhoriyamu uye zvimwe zvinokonzeresa coagulation, saka inogona kushandiswa pakuongorora kushanda kweplatelet.Sodium citrate ine shoma cytotoxicity uye zvakare chimwe chezvikamu zveropa rekuchengetedza mvura mukuwedzerwa ropa.Zvisinei, sodium citrate 6mg inogona anticoagulate 1ml ropa, iro rakasimba alkaline, uye harina kukodzera kuongororwa kweropa uye biochemical test.