Likhopolo tsa mantlha tse amanang le ts'ebetso ea coagulation

Molao-motheo oa anticoagulant le tšebeliso ea li-anticoagulants tse tloaelehileng

1. Heparin ke anticoagulant e khethiloeng bakeng sa ho lemoha lik'hemik'hale tsa mali.Heparin ke mucopolysaccharide e nang le sehlopha sa sulfate, 'me karolelano ea boima ba molek'hule ea mohato o qhalakaneng ke 15000. Molao-motheo oa eona oa anticoagulation ke haholo-holo ho kopana le antithrombin III ho baka liphetoho ho tlhophiso ea antithrombin III le ho potlakisa ho thehoa ha thrombin thrombigu complex ho hlahisa anticoagulation. .Ho phaella moo, heparin e ka thibela thrombin ka thuso ea plasma cofactor (heparin cofactor II).Li-anticoagulants tse tloaelehileng tsa heparin ke sodium, potasiamo, lithium le ammonium salts ea heparin, eo har'a eona lithium heparin e molemo ka ho fetisisa, empa theko ea eona e theko e boima.Matsoai a sodium le potasiamo a tla eketsa litaba tsa sodium le potasiamo maling, 'me letsoai la ammonium le tla eketsa litaba tsa urea nitrogen.Tekanyetso ea heparin bakeng sa anticoagulation hangata ke 10. 0 ~ 12.5 IU/ml mali.Heparin e na le tšitiso e fokolang ea likarolo tsa mali, ha e ame boholo ba lisele tse khubelu tsa mali, 'me ha e bake hemolysis.E loketse tlhahlobo ea lisele, khase ea mali, ho kenella ha plasma, hematocrit le boikemisetso bo akaretsang ba lik'hemik'hale.Leha ho le joalo, heparin e na le phello ea antithrombin 'me ha e loketse tlhahlobo ea mali ea coagulation.Ho feta moo, heparin e feteletseng e ka baka leukocyte aggregation le thrombocytopenia, ka hona ha e tšoanelehe bakeng sa lihlopha tsa leukocyte le palo ea liplatelete, kapa bakeng sa tlhahlobo ea hemostasis Ho phaella moo, heparin anticoagulation e ke ke ea sebelisoa ho etsa litlolo tsa mali, hobane mokokotlo o moputsoa o lefifi o hlaha ka mor'a Wright staining. , e amang ho fokotseha ha tlhahiso ea microscopic.Heparin anticoagulation e lokela ho sebelisoa ka nako e khutšoanyane, ho seng joalo mali a ka kopanya ka mor'a ho behoa nako e telele haholo.

2. letsoai la EDTA.EDTA e ka kopana le Ca2+ maling ho etsa chelate.Ts'ebetso ea ho kopanya e koetsoe 'me mali ha a khone ho kopanya letsoai la EDTA le kenyelletsa letsoai la potasiamo, sodium le lithium.Komiti ea Machabeng ea Boemo ba Hematology e khothalletsa tšebeliso ea EDTA-K2, e nang le solubility e phahameng ka ho fetisisa le lebelo la anticoagulation le potlakileng ka ho fetisisa.Letsoai la EDTA hangata le lokisoa ka tharollo e nang le metsi a nang le karolo e boima ea 15%.Eketsa 1.2mgEDTA ka ml ea mali, ke hore, eketsa 0.04ml ea 15% ea tharollo ea EDTA ka 5ml ea mali.Letsoai la EDTA le ka omisoa ho 100 ℃, 'me phello ea lona ea anticoagulation e lula e sa fetohe letsoai la EDTA ha le ame palo le boholo ba lisele tse tšoeu tsa mali, le na le phello e fokolang ho morphology ea lisele tse khubelu tsa mali, li thibela ho kopana ha platelet,' me li loketse bakeng sa hematological ka kakaretso. ho lemoha.Haeba motsoako oa anticoagulant o phahame haholo, khatello ea osmotic e tla phahama, e leng ho tla etsa hore sele e fokotsehe The pH ea tharollo ea EDTA e na le kamano e kholo le letsoai, 'me pH e tlaase e ka baka ho atolosoa ha lisele.EDTA-K2 e ka atolosa hanyenyane palo ea lisele tse khubelu tsa mali, 'me palo e tloaelehileng ea platelet ka nako e khutšoanyane ka mor'a hore pokello ea mali e tsitsa haholo' me e atisa ho tsitsa ka mor'a halofo ea hora.EDTA-K2 e fokotsehile Ca2+, Mg2+, creatine kinase le alkaline phosphatase.Motsoako o nepahetseng oa EDTA-K2 e ne e le 1. 5mg/ml mali.Haeba ho e-na le mali a fokolang, li-neutrophils li tla ruruha, li-lobule 'me li nyamele, liplatelete li tla ruruha le ho arohana, ho hlahisa likhechana tsa li-platelet tse tloaelehileng, tse tla lebisa liphoso ho liphello tsa tlhahlobo, letsoai la EDTA le ka thibela kapa la kena-kenana le polymerization ea fibrin monomers nakong ea ho thehoa. ea fibrin clots, e sa lokelang bakeng sa ho fumanoa ha mali a coagulation le platelet mosebetsi, leha e le bakeng sa qeto ea khalsiamo, potasiamo, sodium le lintho tse nang le naetrojene.Ho feta moo, EDTA e ka ama ts'ebetso ea li-enzyme tse ling 'me ea thibela lupus erythematosus factor, ka hona ha e loketse ho etsa staining ea histochemical le ho hlahloba mali a lisele tsa lupus erythematosus.

3. Citrate ke haholo-holo sodium citrate.Molao-motheo oa eona oa anticoagulation ke hore o ka kopana le Ca2 + maling ho theha chelate, e le hore Ca2 + e lahleheloe ke mosebetsi oa eona oa ho kopanya 'me mokhoa oa ho kopanya o koetsoe, kahoo o thibela ho kopana ha mali.Sodium citrate e na le mefuta e 'meli ea likristale, Na3C6H5O7 · 2H2O le 2Na3C6H5O7 · 11H2O, hangata 3.8% kapa 3 le ea pele.2% tharollo ea metsi, e kopantsoeng le mali ka molumo oa 1: 9.Liteko tse ngata tsa coagulation li ka ba anticoagulated ka sodium citrate, e thusang ho tsitsisa ha factor V le factor VIII, 'me e na le tšusumetso e fokolang ho palo e tloaelehileng ea platelet le lintlha tse ling tsa coagulation, kahoo e ka sebelisoa bakeng sa tlhahlobo ea mosebetsi oa platelet.Sodium citrate e na le cytotoxicity e fokolang hape ke e 'ngoe ea likarolo tsa mokelikeli oa tlhokomelo ea mali tšelo ea mali.Leha ho le joalo, sodium citrate 6mg e ka thibela mali a 1ml, e leng alkaline e matla, 'me ha e tšoanelehe bakeng sa tlhahlobo ea mali le liteko tsa biochemical.