Sirinjin da za a iya zubarwa – Shafi 2

Sirinjin da za a iya zubarwa - Shafi II

1. Gwajin endotoxin na kwayoyin cuta:

1.1 Shirye-shiryen gwaji:

Tasoshin da aka yi amfani da su a gwajin suna buƙatar a yi musu magani.Hanyar gama gari ita ce busasshen gasa a 180 ℃ na awanni 2.Dole ne a hana gurɓacewar ƙwayoyin cuta yayin aikin gwaji.

Ruwa don gwajin endotoxin na kwayan cuta yana nufin ruwa mai haifuwa don allura wanda baya haifar da amsawar iska tare da reagent na LAL tare da azanci na 0.03EU/ml ko mafi girma a ƙarƙashin 37 ℃ ± 1 ℃ na awanni 24.

1.2 Hanyar gwaji:

Ɗauki 8 ampoules na asali na 0.1 ml / yanki na lysate lysate, 2 daga cikinsu an haɗa su tare da 0.1 ml na maganin gwaji a matsayin gwajin gwajin, kuma 2 daga cikinsu an kara da 0.1 ml na 2.0 wanda aka yi da ma'auni na aikin endotoxin na kwayan cuta tare da ruwa. don gwajin endotoxin na kwayan cuta λ Ana amfani da maida hankali na maganin endotoxin azaman bututu mai inganci.Ƙara 0.1ml na ruwan gwajin endotoxin na kwayan cuta zuwa bututu 2 azaman bututun sarrafawa mara kyau.Ƙara 0.1ml na labarin gwaji tabbatacce bayani mai kulawa zuwa 2 tubes λ Ƙaddamar da maganin endotoxin] azaman ingantaccen bututun sarrafawa na labarin gwaji.A hankali a haxa maganin a cikin bututun gwajin, rufe bututun, a tsaye a saka shi a cikin akwatin wanka na ruwa 37 ℃ 1 ℃, sannan a fitar da shi a hankali bayan 60 ± 2min na adana zafi.Guji sakamakon tabbataccen ƙarya da girgiza ke haifarwa yayin adana zafi da ɗaukar bututu.

Hukuncin sakamako:

A hankali fitar da bututun gwajin kuma a hankali juya shi don 1800. Idan gel a cikin bututun bai lalace ba kuma bai zamewa daga bangon bututu ba, sakamako ne mai kyau kuma an rubuta shi azaman (+);Idan gel ɗin ba za a iya kiyaye shi ba kuma ya zamewa daga bangon bututu, ana yin rikodin mummunan sakamakon azaman (-).