Nga Puawai Tukua - Apitihanga II
1. Whakamātautau huakita endotoxin:
1.1 Whakaritenga whakamatautau:
Ko nga oko i whakamahia i roto i te whakamatautau me rongoa.Ko te tikanga ko te whakamaroke i te tunu i te 180 ℃ mo te 2 haora.Me aukati te poke moroiti i te wa e mahi ana te whakamatautau.
Ko te wai mo te whakamatautau endotoxin huakita e pa ana ki te wai kua whakahoroa mo te werohanga kaore e puta te tauhohenga whakaahuru ki te reagent LAL me te 0.03EU/ml teitei ake ranei i raro i te 37 ℃ ± 1 ℃ mo te 24 haora.
1.2 Tikanga whakamatautau:
Tangohia 8 ampoules taketake o 0.1 ml / wahi o te lysate lysate, 2 o e kua tāpiri ki te 0.1 ml o te otinga whakamātautau rite te ipuipu, me te 2 o e tāpiri ki te 0.1 ml o 2.0 hanga o te paerewa mahi o te endotoxin kitakita ki te wai. mo te whakamatautau endotoxin huakita λ Ko te kukū o te otinga endotoxin ka whakamahia hei ngongo whakahaere pai.Tāpirihia te 0.1ml o te wai whakamatautau huakita endotoxin ki nga ngongo e 2 hei ngongo whakahaere kino.Taapirihia te 0.1ml o te tuhinga whakamatautau otinga mana pai ki nga ngongo e 2 λ Te Kohanga o te otinga endotoxin] hei ngongo whakahaere pai o te tuhinga whakamatautau.Whakaranu marietia te otinga ki roto i te ipuipu, katia te puha, ka maka poutū ki roto i te pouaka kaukau wai 37 ℃ ± 1 ℃, ka ata tango i muri i te 60 ± 2 meneti o te whakamahana wera.A ape i nga hua pai teka na te wiri i te wa e tiakina ana te wera me te tango ngongo.
Whakatau Whakatau:
Me ata tango i te ipuipu whakamatautau ka ata huri whakahurihuri mo te 1800. Ki te kore te reera o roto i te ngongo e paheke, kare hoki e paheke mai i te pakitara ngongo, he hua pai ka tuhia hei (+);Ki te kore e taea te pupuri tonu te rae ka paheke mai i te pakitara ngongo, ka tuhia te hua kino ki te (-).
(1) Ko nga ipuipu whakamatautau e rua he (-), me whakaaro hei whakatutuki i nga whakaritenga;Mena he (+) raua tokorua, ka kiia he kore tohu.
(2) Mena he (+) tetahi ngongo whakamatautau me tetahi ko (-), tangohia etahi atu ngongo whakamatautau kia wha hei whakamatautau ano i runga i te tikanga o runga ake nei, a ko tetahi o nga ngongo e wha he (+), ka kiia he koretake.
(3) Mena he (-) te ngongo whakahaere pai, he (-) ranei te tauira whakamatautau he (+), he muhu te whakamatautau.
Nga Puawai Tukua - Apiti III
1. Kotahi te wa ka tangohia te mahere tauira mo te tirotiro i ia wa.Ko te whakarōpūtanga o nga hua kore e rite ana, te roopu whakamatautau, nga mea tirotiro, te taumata whakahirahira, te RQL (te taumata kounga kore e rite) me te mahere tauira kua tohua ki te ripanga e whai ake nei.
Tuhipoka: Ko te ihirangi cadmium i roto i te 5.14.1 o te paerewa ka tukuna mo te tirotiro.
2. Kotahi te wa ka tangohia te mahere tauira mo te tirotiro roopu.Ka tiimata mai i te mahere tauira tirotiro noa.Ko te whakarōpūtanga, nga mea tirotiro, te taumata tirotiro (IL) me te taumata o te kounga tohu (AQL) o nga hua kore e rite ana kua tohua ki te ripanga e whai ake nei.
Wā tuku: Oketopa-06-2022
